Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis
Magnesium (Mg) is one of the essential nutrients for all living organisms. Plants acquire Mg from the environment and distribute within their bodies in the ionic form via Mg2+-permeable transporters. In Arabidopsis, the plasma membrane-localized magnesium transporter MGT6 mediates Mg2+ uptake under...
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00274/full |
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| author | Yu-Wei Yan Yu-Wei Yan Dan-Dan Mao Dan-Dan Mao Lei Yang Jin-Liang Qi Jin-Liang Qi Xin-Xin Zhang Xin-Xin Zhang Qing-Lin Tang Qing-Lin Tang Yang-Ping Li Yang-Ping Li Ren-Jie Tang Sheng Luan |
| author_facet | Yu-Wei Yan Yu-Wei Yan Dan-Dan Mao Dan-Dan Mao Lei Yang Jin-Liang Qi Jin-Liang Qi Xin-Xin Zhang Xin-Xin Zhang Qing-Lin Tang Qing-Lin Tang Yang-Ping Li Yang-Ping Li Ren-Jie Tang Sheng Luan |
| author_sort | Yu-Wei Yan |
| collection | DOAJ |
| description | Magnesium (Mg) is one of the essential nutrients for all living organisms. Plants acquire Mg from the environment and distribute within their bodies in the ionic form via Mg2+-permeable transporters. In Arabidopsis, the plasma membrane-localized magnesium transporter MGT6 mediates Mg2+ uptake under Mg-limited conditions, and therefore is important for the plant adaptation to low-Mg environment. In this study, we further assessed the physiological function of MGT6 using a knockout T-DNA insertional mutant allele. We found that MGT6 was required for normal plant growth during various developmental stages when the environmental Mg2+ was low. Interestingly, in addition to the hypersensitivity to Mg2+ limitation, mgt6 mutants displayed dramatic growth defects when external Mg2+ was in excess. Compared with wild-type plants, mgt6 mutants generally contained less Mg2+ under both low and high external Mg2+ conditions. Reciprocal grafting experiments further underpinned a role of MGT6 in a shoot-based mechanism for detoxifying excessive Mg2+ in the environment. Moreover, we found that mgt6 mgt7 double mutant showed more severe phenotypes compared with single mutants under both low- and high-Mg2+ stress conditions, suggesting that these two MGT-type transporters play an additive role in controlling plant Mg2+ homeostasis under a wide range of external Mg2+ concentrations. |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T22:42:57Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b22a6209c49c4952ba2f24c60c6bf3c12022-12-22T02:26:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-03-01910.3389/fpls.2018.00274347703Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in ArabidopsisYu-Wei Yan0Yu-Wei Yan1Dan-Dan Mao2Dan-Dan Mao3Lei Yang4Jin-Liang Qi5Jin-Liang Qi6Xin-Xin Zhang7Xin-Xin Zhang8Qing-Lin Tang9Qing-Lin Tang10Yang-Ping Li11Yang-Ping Li12Ren-Jie Tang13Sheng Luan14Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Maize Research Institute of Sichuan Agricultural University, Chengdu, ChinaNanjing University–Nanjing Forestry University Joint Institute for Plant Molecular Biology, State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaNanjing University–Nanjing Forestry University Joint Institute for Plant Molecular Biology, State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, ChinaDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesNanjing University–Nanjing Forestry University Joint Institute for Plant Molecular Biology, State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, ChinaDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesKey Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin, ChinaDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesKey Laboratory of Horticulture Science for Southern Mountainous Regions, Southwest University, Chongqing, ChinaDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesKey Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Maize Research Institute of Sichuan Agricultural University, Chengdu, ChinaDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesMagnesium (Mg) is one of the essential nutrients for all living organisms. Plants acquire Mg from the environment and distribute within their bodies in the ionic form via Mg2+-permeable transporters. In Arabidopsis, the plasma membrane-localized magnesium transporter MGT6 mediates Mg2+ uptake under Mg-limited conditions, and therefore is important for the plant adaptation to low-Mg environment. In this study, we further assessed the physiological function of MGT6 using a knockout T-DNA insertional mutant allele. We found that MGT6 was required for normal plant growth during various developmental stages when the environmental Mg2+ was low. Interestingly, in addition to the hypersensitivity to Mg2+ limitation, mgt6 mutants displayed dramatic growth defects when external Mg2+ was in excess. Compared with wild-type plants, mgt6 mutants generally contained less Mg2+ under both low and high external Mg2+ conditions. Reciprocal grafting experiments further underpinned a role of MGT6 in a shoot-based mechanism for detoxifying excessive Mg2+ in the environment. Moreover, we found that mgt6 mgt7 double mutant showed more severe phenotypes compared with single mutants under both low- and high-Mg2+ stress conditions, suggesting that these two MGT-type transporters play an additive role in controlling plant Mg2+ homeostasis under a wide range of external Mg2+ concentrations.http://journal.frontiersin.org/article/10.3389/fpls.2018.00274/fullMg2+ transporterMg2+ homeostasisArabidopsisMGT6MGT7 |
| spellingShingle | Yu-Wei Yan Yu-Wei Yan Dan-Dan Mao Dan-Dan Mao Lei Yang Jin-Liang Qi Jin-Liang Qi Xin-Xin Zhang Xin-Xin Zhang Qing-Lin Tang Qing-Lin Tang Yang-Ping Li Yang-Ping Li Ren-Jie Tang Sheng Luan Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis Frontiers in Plant Science Mg2+ transporter Mg2+ homeostasis Arabidopsis MGT6 MGT7 |
| title | Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis |
| title_full | Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis |
| title_fullStr | Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis |
| title_full_unstemmed | Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis |
| title_short | Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis |
| title_sort | magnesium transporter mgt6 plays an essential role in maintaining magnesium homeostasis and regulating high magnesium tolerance in arabidopsis |
| topic | Mg2+ transporter Mg2+ homeostasis Arabidopsis MGT6 MGT7 |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00274/full |
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