Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals
The advent of CRISPR/Cas9 technology has revolutionized genome editing, enabling the attainment of once-unimaginable goals. CRISPR/Cas’s groundbreaking attributes lie in its simplicity, versatility, universality, and independence from customized DNA-protein systems, erasing the need for specialized...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/6/3271 |
| _version_ | 1797240703372230656 |
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| author | Aurélien Devillars Gabriele Magon Carlotta Pirrello Fabio Palumbo Silvia Farinati Gianni Barcaccia Margherita Lucchin Alessandro Vannozzi |
| author_facet | Aurélien Devillars Gabriele Magon Carlotta Pirrello Fabio Palumbo Silvia Farinati Gianni Barcaccia Margherita Lucchin Alessandro Vannozzi |
| author_sort | Aurélien Devillars |
| collection | DOAJ |
| description | The advent of CRISPR/Cas9 technology has revolutionized genome editing, enabling the attainment of once-unimaginable goals. CRISPR/Cas’s groundbreaking attributes lie in its simplicity, versatility, universality, and independence from customized DNA-protein systems, erasing the need for specialized expertise and broadening its scope of applications. It is therefore more and more used for genome modification including the generation of mutants. Beyond such editing scopes, the recent development of novel or modified Cas-based systems has spawned an array of additional biotechnological tools, empowering both fundamental and applied research. Precisely targeting DNA or RNA sequences, the CRISPR/Cas system has been harnessed in fields as diverse as gene regulation, deepening insights into gene expression, epigenetic changes, genome spatial organization, and chromatin dynamics. Furthermore, it aids in genome imaging and sequencing, as well as effective identification and countering of viral pathogens in plants and animals. All in all, the non-editing aspect of CRISPR/Cas exhibits tremendous potential across diverse domains, including diagnostics, biotechnology, and fundamental research. This article reviews and critically evaluates the primary CRISPR/Cas-based tools developed for plants and animals, underlining their transformative impact. |
| first_indexed | 2024-04-24T18:11:39Z |
| format | Article |
| id | doaj.art-b22c99025b3c46008c072334864c4cc6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:11:39Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b22c99025b3c46008c072334864c4cc62024-03-27T13:45:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256327110.3390/ijms25063271Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and AnimalsAurélien Devillars0Gabriele Magon1Carlotta Pirrello2Fabio Palumbo3Silvia Farinati4Gianni Barcaccia5Margherita Lucchin6Alessandro Vannozzi7Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, PD, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, PD, ItalyResearch and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, TN, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, PD, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, PD, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, PD, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, PD, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, PD, ItalyThe advent of CRISPR/Cas9 technology has revolutionized genome editing, enabling the attainment of once-unimaginable goals. CRISPR/Cas’s groundbreaking attributes lie in its simplicity, versatility, universality, and independence from customized DNA-protein systems, erasing the need for specialized expertise and broadening its scope of applications. It is therefore more and more used for genome modification including the generation of mutants. Beyond such editing scopes, the recent development of novel or modified Cas-based systems has spawned an array of additional biotechnological tools, empowering both fundamental and applied research. Precisely targeting DNA or RNA sequences, the CRISPR/Cas system has been harnessed in fields as diverse as gene regulation, deepening insights into gene expression, epigenetic changes, genome spatial organization, and chromatin dynamics. Furthermore, it aids in genome imaging and sequencing, as well as effective identification and countering of viral pathogens in plants and animals. All in all, the non-editing aspect of CRISPR/Cas exhibits tremendous potential across diverse domains, including diagnostics, biotechnology, and fundamental research. This article reviews and critically evaluates the primary CRISPR/Cas-based tools developed for plants and animals, underlining their transformative impact.https://www.mdpi.com/1422-0067/25/6/3271CRISPR/Casimaginggene regulationNGSvirusesCRISPR/Cas9 |
| spellingShingle | Aurélien Devillars Gabriele Magon Carlotta Pirrello Fabio Palumbo Silvia Farinati Gianni Barcaccia Margherita Lucchin Alessandro Vannozzi Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals International Journal of Molecular Sciences CRISPR/Cas imaging gene regulation NGS viruses CRISPR/Cas9 |
| title | Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals |
| title_full | Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals |
| title_fullStr | Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals |
| title_full_unstemmed | Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals |
| title_short | Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals |
| title_sort | not only editing a cas cade of crispr cas based tools for functional genomics in plants and animals |
| topic | CRISPR/Cas imaging gene regulation NGS viruses CRISPR/Cas9 |
| url | https://www.mdpi.com/1422-0067/25/6/3271 |
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