A semi high-throughput whole blood-based flow cytometry assay to detect and monitor Bordetella pertussis-specific Th1, Th2 and Th17 responses

A semi high-throughput whole blood-based flow cytometry assay to detect and monitor Bordetella pertussis-specific Th1, Th2 and Th17 responses

IntroductionThe characterization of B. pertussis (Bp) antigen-specific CD4+ T cell cytokine responses should be included in the evaluation of immunogenicity of pertussis vaccines but is often hindered by the lack of standardized robust assays.MethodsTo overcome this limitation, we developed a two-st...

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Bibliographic Details
Main Authors: Véronique Corbière, Eleonora E. Lambert, Marine Rodesch, Jacqueline A. M. van Gaans-van den Brink, Alicja Misiak, Elles Simonetti, Anne Van Praet, Audrey Godefroid, Dimitri A. Diavatopoulos, Cécile A. C. M. van Els, Françoise Mascart, PERISCOPE WP5 Task 7 working group
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-02-01
Series:Frontiers in Immunology
Subjects:
Bordetella pertussis
vaccine
whole blood assay
Th1/Th2/Th17
intracellular cytokine staining
flow cytometry
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2023.1101366/full

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