Alkaloids from Tetrastigma hemsleyanum and Their Anti-Inflammatory Effects on LPS-Induced RAW264.7 Cells
Alkaloids 1–10 were isolated from the aerial parts of Tetrastigma hemsleyanum (APTH) and obtained from species of the genus Tetrastigma for the first time. The chemical structures of the isolated compounds were identified by NMR, UV, and MS analyses. Their anti-inflammatory activities were...
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MDPI AG
2018-06-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/6/1445 |
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| author | Cai Yi Wang Hyun-Jae Jang Yoo Kyong Han Xiang Dong Su Seung Woong Lee Mun-Chual Rho Heng-Shan Wang Seo Young Yang Young Ho Kim |
| author_facet | Cai Yi Wang Hyun-Jae Jang Yoo Kyong Han Xiang Dong Su Seung Woong Lee Mun-Chual Rho Heng-Shan Wang Seo Young Yang Young Ho Kim |
| author_sort | Cai Yi Wang |
| collection | DOAJ |
| description | Alkaloids 1–10 were isolated from the aerial parts of Tetrastigma hemsleyanum (APTH) and obtained from species of the genus Tetrastigma for the first time. The chemical structures of the isolated compounds were identified by NMR, UV, and MS analyses. Their anti-inflammatory activities were investigated by measuring nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Among all the isolates, compounds 6, 7 and 10 showed potent inhibitory activity against LPS-stimulated NO production in RAW264.7 cells (IC50: 31.9, 25.2 and 6.3 μM, respectively). Furthermore, APTH and S-(−)-trolline (10) inhibited induction of inflammatory cytokines or mediators such as interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) via suppression of nuclear factor κB (NF-κB) translocation into the nucleus. In addition, 10 suppressed extracellular signal-regulated protein kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) phosphorylation in a dose-dependent manner. These results conclusively demonstrated that compound 10 displays anti-inflammatory activity via suppression of NF-κB activation and the ERK-MAPK signaling pathway in LPS-stimulated RAW264.7 cells. |
| first_indexed | 2024-12-22T13:50:57Z |
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| id | doaj.art-b230eb043ec84663b46065715d03d0b1 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T13:50:57Z |
| publishDate | 2018-06-01 |
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| series | Molecules |
| spelling | doaj.art-b230eb043ec84663b46065715d03d0b12022-12-21T18:23:39ZengMDPI AGMolecules1420-30492018-06-01236144510.3390/molecules23061445molecules23061445Alkaloids from Tetrastigma hemsleyanum and Their Anti-Inflammatory Effects on LPS-Induced RAW264.7 CellsCai Yi Wang0Hyun-Jae Jang1Yoo Kyong Han2Xiang Dong Su3Seung Woong Lee4Mun-Chual Rho5Heng-Shan Wang6Seo Young Yang7Young Ho Kim8College of Pharmacy, Chungnam National University, Daejeon 34134, KoreaImmunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaImmunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, KoreaImmunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, KoreaState Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, ChinaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaAlkaloids 1–10 were isolated from the aerial parts of Tetrastigma hemsleyanum (APTH) and obtained from species of the genus Tetrastigma for the first time. The chemical structures of the isolated compounds were identified by NMR, UV, and MS analyses. Their anti-inflammatory activities were investigated by measuring nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Among all the isolates, compounds 6, 7 and 10 showed potent inhibitory activity against LPS-stimulated NO production in RAW264.7 cells (IC50: 31.9, 25.2 and 6.3 μM, respectively). Furthermore, APTH and S-(−)-trolline (10) inhibited induction of inflammatory cytokines or mediators such as interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) via suppression of nuclear factor κB (NF-κB) translocation into the nucleus. In addition, 10 suppressed extracellular signal-regulated protein kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) phosphorylation in a dose-dependent manner. These results conclusively demonstrated that compound 10 displays anti-inflammatory activity via suppression of NF-κB activation and the ERK-MAPK signaling pathway in LPS-stimulated RAW264.7 cells.http://www.mdpi.com/1420-3049/23/6/1445Tetrastigma hemsleyanumalkaloidNO productionanti-inflammatory activity |
| spellingShingle | Cai Yi Wang Hyun-Jae Jang Yoo Kyong Han Xiang Dong Su Seung Woong Lee Mun-Chual Rho Heng-Shan Wang Seo Young Yang Young Ho Kim Alkaloids from Tetrastigma hemsleyanum and Their Anti-Inflammatory Effects on LPS-Induced RAW264.7 Cells Molecules Tetrastigma hemsleyanum alkaloid NO production anti-inflammatory activity |
| title | Alkaloids from Tetrastigma hemsleyanum and Their Anti-Inflammatory Effects on LPS-Induced RAW264.7 Cells |
| title_full | Alkaloids from Tetrastigma hemsleyanum and Their Anti-Inflammatory Effects on LPS-Induced RAW264.7 Cells |
| title_fullStr | Alkaloids from Tetrastigma hemsleyanum and Their Anti-Inflammatory Effects on LPS-Induced RAW264.7 Cells |
| title_full_unstemmed | Alkaloids from Tetrastigma hemsleyanum and Their Anti-Inflammatory Effects on LPS-Induced RAW264.7 Cells |
| title_short | Alkaloids from Tetrastigma hemsleyanum and Their Anti-Inflammatory Effects on LPS-Induced RAW264.7 Cells |
| title_sort | alkaloids from tetrastigma hemsleyanum and their anti inflammatory effects on lps induced raw264 7 cells |
| topic | Tetrastigma hemsleyanum alkaloid NO production anti-inflammatory activity |
| url | http://www.mdpi.com/1420-3049/23/6/1445 |
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