Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes
Acute lymphoblastic leukemia (ALL) is the most common type of malignant neoplasms in the pediatric population. B-cell precursor ALLs (BCP-ALLs) are derived from the progenitors of B lymphocytes. Traditionally, risk factors stratifying therapy in ALL patients included age at diagnosis, initial leukoc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/21/5483 |
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| author | Patrycja Leszczenko Aleksandra Borek-Dorosz Anna Maria Nowakowska Adriana Adamczyk Sviatlana Kashyrskaya Justyna Jakubowska Marta Ząbczyńska Agata Pastorczak Kinga Ostrowska Malgorzata Baranska Katarzyna Maria Marzec Katarzyna Majzner |
| author_facet | Patrycja Leszczenko Aleksandra Borek-Dorosz Anna Maria Nowakowska Adriana Adamczyk Sviatlana Kashyrskaya Justyna Jakubowska Marta Ząbczyńska Agata Pastorczak Kinga Ostrowska Malgorzata Baranska Katarzyna Maria Marzec Katarzyna Majzner |
| author_sort | Patrycja Leszczenko |
| collection | DOAJ |
| description | Acute lymphoblastic leukemia (ALL) is the most common type of malignant neoplasms in the pediatric population. B-cell precursor ALLs (BCP-ALLs) are derived from the progenitors of B lymphocytes. Traditionally, risk factors stratifying therapy in ALL patients included age at diagnosis, initial leukocytosis, and the response to chemotherapy. Currently, treatment intensity is modified according to the presence of specific gene alterations in the leukemic genome. Raman imaging is a promising diagnostic tool, which enables the molecular characterization of cells and differentiation of subtypes of leukemia in clinical samples. This study aimed to characterize and distinguish cells isolated from the bone marrow of patients suffering from three subtypes of BCP-ALL, defined by gene rearrangements, i.e., <i>BCR-ABL1</i> <i>(Philadelphia</i>-positive, t(9;22)), <i>TEL-AML1</i> (t(12;21)) and <i>TCF3-PBX1</i> (t(1;19)), using single-cell Raman imaging combined with multivariate statistical analysis. Spectra collected from clinical samples were compared with single-cell spectra of B-cells collected from healthy donors, constituting the control group. We demonstrated that Raman spectra of normal B cells strongly differ from spectra of their malignant counterparts, especially in the intensity of bands, which can be assigned to nucleic acids. We also showed that the identification of leukemia subtypes could be automated with the use of chemometric methods. Results prove the clinical suitability of Raman imaging for the identification of spectroscopic markers characterizing leukemia cells. |
| first_indexed | 2024-03-10T06:05:49Z |
| format | Article |
| id | doaj.art-b23478871ab340618ebc3930ee9d4788 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T06:05:49Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-b23478871ab340618ebc3930ee9d47882023-11-22T20:35:55ZengMDPI AGCancers2072-66942021-10-011321548310.3390/cancers13215483Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) SubtypesPatrycja Leszczenko0Aleksandra Borek-Dorosz1Anna Maria Nowakowska2Adriana Adamczyk3Sviatlana Kashyrskaya4Justyna Jakubowska5Marta Ząbczyńska6Agata Pastorczak7Kinga Ostrowska8Malgorzata Baranska9Katarzyna Maria Marzec10Katarzyna Majzner11Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandDepartment of Pediatrics, Oncology and Hematology, Medical University of Lodz, Sporna 36/50, 91-738 Lodz, PolandDepartment of Pediatrics, Oncology and Hematology, Medical University of Lodz, Sporna 36/50, 91-738 Lodz, PolandDepartment of Pediatrics, Oncology and Hematology, Medical University of Lodz, Sporna 36/50, 91-738 Lodz, PolandDepartment of Pediatrics, Oncology and Hematology, Medical University of Lodz, Sporna 36/50, 91-738 Lodz, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandLukasiewicz Research Network—Krakow Institute of Technology, Zakopiańska 73, 30-418 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandAcute lymphoblastic leukemia (ALL) is the most common type of malignant neoplasms in the pediatric population. B-cell precursor ALLs (BCP-ALLs) are derived from the progenitors of B lymphocytes. Traditionally, risk factors stratifying therapy in ALL patients included age at diagnosis, initial leukocytosis, and the response to chemotherapy. Currently, treatment intensity is modified according to the presence of specific gene alterations in the leukemic genome. Raman imaging is a promising diagnostic tool, which enables the molecular characterization of cells and differentiation of subtypes of leukemia in clinical samples. This study aimed to characterize and distinguish cells isolated from the bone marrow of patients suffering from three subtypes of BCP-ALL, defined by gene rearrangements, i.e., <i>BCR-ABL1</i> <i>(Philadelphia</i>-positive, t(9;22)), <i>TEL-AML1</i> (t(12;21)) and <i>TCF3-PBX1</i> (t(1;19)), using single-cell Raman imaging combined with multivariate statistical analysis. Spectra collected from clinical samples were compared with single-cell spectra of B-cells collected from healthy donors, constituting the control group. We demonstrated that Raman spectra of normal B cells strongly differ from spectra of their malignant counterparts, especially in the intensity of bands, which can be assigned to nucleic acids. We also showed that the identification of leukemia subtypes could be automated with the use of chemometric methods. Results prove the clinical suitability of Raman imaging for the identification of spectroscopic markers characterizing leukemia cells.https://www.mdpi.com/2072-6694/13/21/5483Raman spectroscopyacute lymphoblastic leukemia (ALL)fusion geneschemometric techniques<i>BCR-ABL1</i><i>TCF-PBX1</i> |
| spellingShingle | Patrycja Leszczenko Aleksandra Borek-Dorosz Anna Maria Nowakowska Adriana Adamczyk Sviatlana Kashyrskaya Justyna Jakubowska Marta Ząbczyńska Agata Pastorczak Kinga Ostrowska Malgorzata Baranska Katarzyna Maria Marzec Katarzyna Majzner Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes Cancers Raman spectroscopy acute lymphoblastic leukemia (ALL) fusion genes chemometric techniques <i>BCR-ABL1</i> <i>TCF-PBX1</i> |
| title | Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes |
| title_full | Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes |
| title_fullStr | Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes |
| title_full_unstemmed | Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes |
| title_short | Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes |
| title_sort | towards raman based screening of acute lymphoblastic leukemia type b b all subtypes |
| topic | Raman spectroscopy acute lymphoblastic leukemia (ALL) fusion genes chemometric techniques <i>BCR-ABL1</i> <i>TCF-PBX1</i> |
| url | https://www.mdpi.com/2072-6694/13/21/5483 |
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