An Asymmetric Microfluidic/Chitosan Device for Sustained Drug Release in Guided Bone Regeneration Applications
One of the major challenges of guided bone regeneration (GBR) is infections caused by pathogen colonization at wound sites. In this paper, an asymmetric microfluidic/chitosan device was developed to release drugs to inhibit infections and to ensure that guided bone regeneration can be realized. The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/847 |
| _version_ | 1797474800813211648 |
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| author | Xin Shi Beibei Ma Hongyu Chen Wei Tan Shiqing Ma Guorui Zhu |
| author_facet | Xin Shi Beibei Ma Hongyu Chen Wei Tan Shiqing Ma Guorui Zhu |
| author_sort | Xin Shi |
| collection | DOAJ |
| description | One of the major challenges of guided bone regeneration (GBR) is infections caused by pathogen colonization at wound sites. In this paper, an asymmetric microfluidic/chitosan device was developed to release drugs to inhibit infections and to ensure that guided bone regeneration can be realized. The microfluidic technique was introduced into the GBR membrane for the first time, which demonstrated more controllable drug release, more flexible clinical use and had a lower cost compared with surface treatments and embedded nanoparticles. Based on the theory of diffusion and Fick’s first law, the contact area and concentration gradient were adjusted to realize sustained drug release. The standard deviation of minocycline release over 5 days was only 12.7%, which was lower than the joint effect of porous chitosan discs and nanospheres. The in vitro experiments against <i>E. coli</i> and Streptococcus mutans showed the excellent antibacterial performance of the device (>95%). The in vitro experiments for fibroblasts at the microfluidic side and osteoblasts at the chitosan side showed the satisfactory biocompatibility and the ability of the device to enhance bone regeneration. Therefore, this microfluidic/chitosan device is a promising therapeutic approach to prevent infection and guide bone regeneration. |
| first_indexed | 2024-03-09T20:36:15Z |
| format | Article |
| id | doaj.art-b236452efe324ec097ae39d4bd22a150 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:36:15Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-b236452efe324ec097ae39d4bd22a1502023-11-23T23:11:38ZengMDPI AGBiosensors2079-63742022-10-01121084710.3390/bios12100847An Asymmetric Microfluidic/Chitosan Device for Sustained Drug Release in Guided Bone Regeneration ApplicationsXin Shi0Beibei Ma1Hongyu Chen2Wei Tan3Shiqing Ma4Guorui Zhu5School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaDepartment of Stomatology, The Second Hospital of Tianjin Medical University, Tianjin 300211, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaOne of the major challenges of guided bone regeneration (GBR) is infections caused by pathogen colonization at wound sites. In this paper, an asymmetric microfluidic/chitosan device was developed to release drugs to inhibit infections and to ensure that guided bone regeneration can be realized. The microfluidic technique was introduced into the GBR membrane for the first time, which demonstrated more controllable drug release, more flexible clinical use and had a lower cost compared with surface treatments and embedded nanoparticles. Based on the theory of diffusion and Fick’s first law, the contact area and concentration gradient were adjusted to realize sustained drug release. The standard deviation of minocycline release over 5 days was only 12.7%, which was lower than the joint effect of porous chitosan discs and nanospheres. The in vitro experiments against <i>E. coli</i> and Streptococcus mutans showed the excellent antibacterial performance of the device (>95%). The in vitro experiments for fibroblasts at the microfluidic side and osteoblasts at the chitosan side showed the satisfactory biocompatibility and the ability of the device to enhance bone regeneration. Therefore, this microfluidic/chitosan device is a promising therapeutic approach to prevent infection and guide bone regeneration.https://www.mdpi.com/2079-6374/12/10/847microfluidicdrug releaseguided bone regeneration |
| spellingShingle | Xin Shi Beibei Ma Hongyu Chen Wei Tan Shiqing Ma Guorui Zhu An Asymmetric Microfluidic/Chitosan Device for Sustained Drug Release in Guided Bone Regeneration Applications Biosensors microfluidic drug release guided bone regeneration |
| title | An Asymmetric Microfluidic/Chitosan Device for Sustained Drug Release in Guided Bone Regeneration Applications |
| title_full | An Asymmetric Microfluidic/Chitosan Device for Sustained Drug Release in Guided Bone Regeneration Applications |
| title_fullStr | An Asymmetric Microfluidic/Chitosan Device for Sustained Drug Release in Guided Bone Regeneration Applications |
| title_full_unstemmed | An Asymmetric Microfluidic/Chitosan Device for Sustained Drug Release in Guided Bone Regeneration Applications |
| title_short | An Asymmetric Microfluidic/Chitosan Device for Sustained Drug Release in Guided Bone Regeneration Applications |
| title_sort | asymmetric microfluidic chitosan device for sustained drug release in guided bone regeneration applications |
| topic | microfluidic drug release guided bone regeneration |
| url | https://www.mdpi.com/2079-6374/12/10/847 |
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