Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus Infection
Hepatitis B, a persistent inflammatory liver condition, stands as a significant global health issue. In Romania, the prevalence of chronic hepatitis B virus (CHB) infection ranks among the highest in the European Union. The HLA genotype significantly impacts hepatitis B virus infection progression,...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/46/2/67 |
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| author | Adriana Tălăngescu Bogdan Calenic Dan Florin Mihăilescu Maria Tizu Ion Marunțelu Alexandra E. Constantinescu Ileana Constantinescu |
| author_facet | Adriana Tălăngescu Bogdan Calenic Dan Florin Mihăilescu Maria Tizu Ion Marunțelu Alexandra E. Constantinescu Ileana Constantinescu |
| author_sort | Adriana Tălăngescu |
| collection | DOAJ |
| description | Hepatitis B, a persistent inflammatory liver condition, stands as a significant global health issue. In Romania, the prevalence of chronic hepatitis B virus (CHB) infection ranks among the highest in the European Union. The HLA genotype significantly impacts hepatitis B virus infection progression, indicating that certain HLA variants can affect the infection’s outcome. The primary goal of the present work is to identify HLA alleles and specific amino acid residues linked to hepatitis B within the Romanian population. The study enrolled 247 patients with chronic hepatitis B; HLA typing was performed using next-generation sequencing. This study’s main findings include the identification of certain HLA alleles, such as DQB1*06:03:01, DRB1*13:01:01, DQB1*06:02:01, DQA1*01:03:01, DRB5*01:01:01, and DRB1*15:01:01, which exhibit a significant protective effect against HBV. Additionally, the amino acid residue alanine at DQB1_38 is associated with a protective role, while valine presence may signal an increased risk of hepatitis B. The present findings are important in addressing the urgent need for improved methods of diagnosing and managing CHB, particularly when considering the disease’s presence in diverse population groups and geographical regions. |
| first_indexed | 2024-03-07T22:37:57Z |
| format | Article |
| id | doaj.art-b23ec6d66dba49a1b3e08c4e4f8cc58a |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-07T22:37:57Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-b23ec6d66dba49a1b3e08c4e4f8cc58a2024-02-23T15:12:34ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-01-014621064107710.3390/cimb46020067Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus InfectionAdriana Tălăngescu0Bogdan Calenic1Dan Florin Mihăilescu2Maria Tizu3Ion Marunțelu4Alexandra E. Constantinescu5Ileana Constantinescu6Immunology and Transplant Immunology, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Avenue, 022328 Bucharest, RomaniaImmunology and Transplant Immunology, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Avenue, 022328 Bucharest, RomaniaDepartment of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, Splaiul Independenței Street, No. 91–95, 050095 Bucharest, RomaniaImmunology and Transplant Immunology, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Avenue, 022328 Bucharest, RomaniaImmunology and Transplant Immunology, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Avenue, 022328 Bucharest, RomaniaImmunology and Transplant Immunology, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Avenue, 022328 Bucharest, RomaniaImmunology and Transplant Immunology, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Avenue, 022328 Bucharest, RomaniaHepatitis B, a persistent inflammatory liver condition, stands as a significant global health issue. In Romania, the prevalence of chronic hepatitis B virus (CHB) infection ranks among the highest in the European Union. The HLA genotype significantly impacts hepatitis B virus infection progression, indicating that certain HLA variants can affect the infection’s outcome. The primary goal of the present work is to identify HLA alleles and specific amino acid residues linked to hepatitis B within the Romanian population. The study enrolled 247 patients with chronic hepatitis B; HLA typing was performed using next-generation sequencing. This study’s main findings include the identification of certain HLA alleles, such as DQB1*06:03:01, DRB1*13:01:01, DQB1*06:02:01, DQA1*01:03:01, DRB5*01:01:01, and DRB1*15:01:01, which exhibit a significant protective effect against HBV. Additionally, the amino acid residue alanine at DQB1_38 is associated with a protective role, while valine presence may signal an increased risk of hepatitis B. The present findings are important in addressing the urgent need for improved methods of diagnosing and managing CHB, particularly when considering the disease’s presence in diverse population groups and geographical regions.https://www.mdpi.com/1467-3045/46/2/67next-generation sequencing (NGS)human leukocyte antigens (HLAs)hepatitis B virus (HBV)amino acid residues |
| spellingShingle | Adriana Tălăngescu Bogdan Calenic Dan Florin Mihăilescu Maria Tizu Ion Marunțelu Alexandra E. Constantinescu Ileana Constantinescu Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus Infection Current Issues in Molecular Biology next-generation sequencing (NGS) human leukocyte antigens (HLAs) hepatitis B virus (HBV) amino acid residues |
| title | Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus Infection |
| title_full | Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus Infection |
| title_fullStr | Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus Infection |
| title_full_unstemmed | Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus Infection |
| title_short | Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus Infection |
| title_sort | molecular analysis of hla genes in romanian patients with chronic hepatitis b virus infection |
| topic | next-generation sequencing (NGS) human leukocyte antigens (HLAs) hepatitis B virus (HBV) amino acid residues |
| url | https://www.mdpi.com/1467-3045/46/2/67 |
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