Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity
The hypothalamus is one of the most complex brain structures involved in homeostatic regulation. Defining cell composition and identifying cell-type-specific transcriptional features of the hypothalamus is essential for understanding its functions and related disorders. Here, we report single-cell R...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717303212 |
| _version_ | 1828376062022647808 |
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| author | Renchao Chen Xiaoji Wu Lan Jiang Yi Zhang |
| author_facet | Renchao Chen Xiaoji Wu Lan Jiang Yi Zhang |
| author_sort | Renchao Chen |
| collection | DOAJ |
| description | The hypothalamus is one of the most complex brain structures involved in homeostatic regulation. Defining cell composition and identifying cell-type-specific transcriptional features of the hypothalamus is essential for understanding its functions and related disorders. Here, we report single-cell RNA sequencing results of adult mouse hypothalamus, which defines 11 non-neuronal and 34 neuronal cell clusters with distinct transcriptional signatures. Analyses of cell-type-specific transcriptomes reveal gene expression dynamics underlying oligodendrocyte differentiation and tanycyte subtypes. Additionally, data analysis provides a comprehensive view of neuropeptide expression across hypothalamic neuronal subtypes and uncover Crabp1+ and Pax6+ neuronal populations in specific hypothalamic sub-regions. Furthermore, we found food deprivation exhibited differential transcriptional effects among the different neuronal subtypes, suggesting functional specification of various neuronal subtypes. Thus, the work provides a comprehensive transcriptional perspective of adult hypothalamus, which serves as a valuable resource for dissecting cell-type-specific functions of this complex brain region. |
| first_indexed | 2024-04-14T07:55:01Z |
| format | Article |
| id | doaj.art-b2424a6835ec4a51ad89dc8e709410ea |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-14T07:55:01Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-b2424a6835ec4a51ad89dc8e709410ea2022-12-22T02:05:05ZengElsevierCell Reports2211-12472017-03-0118133227324110.1016/j.celrep.2017.03.004Single-Cell RNA-Seq Reveals Hypothalamic Cell DiversityRenchao Chen0Xiaoji Wu1Lan Jiang2Yi Zhang3Howard Hughes Medical Institute, Boston Children’s Hospital, Boston, MA 02115, USAHoward Hughes Medical Institute, Boston Children’s Hospital, Boston, MA 02115, USAHoward Hughes Medical Institute, Boston Children’s Hospital, Boston, MA 02115, USAHoward Hughes Medical Institute, Boston Children’s Hospital, Boston, MA 02115, USAThe hypothalamus is one of the most complex brain structures involved in homeostatic regulation. Defining cell composition and identifying cell-type-specific transcriptional features of the hypothalamus is essential for understanding its functions and related disorders. Here, we report single-cell RNA sequencing results of adult mouse hypothalamus, which defines 11 non-neuronal and 34 neuronal cell clusters with distinct transcriptional signatures. Analyses of cell-type-specific transcriptomes reveal gene expression dynamics underlying oligodendrocyte differentiation and tanycyte subtypes. Additionally, data analysis provides a comprehensive view of neuropeptide expression across hypothalamic neuronal subtypes and uncover Crabp1+ and Pax6+ neuronal populations in specific hypothalamic sub-regions. Furthermore, we found food deprivation exhibited differential transcriptional effects among the different neuronal subtypes, suggesting functional specification of various neuronal subtypes. Thus, the work provides a comprehensive transcriptional perspective of adult hypothalamus, which serves as a valuable resource for dissecting cell-type-specific functions of this complex brain region.http://www.sciencedirect.com/science/article/pii/S2211124717303212single-cell RNA-seqhypothalamuscell heterogeneityoligodendrocytetanycyteneuron diversity |
| spellingShingle | Renchao Chen Xiaoji Wu Lan Jiang Yi Zhang Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity Cell Reports single-cell RNA-seq hypothalamus cell heterogeneity oligodendrocyte tanycyte neuron diversity |
| title | Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity |
| title_full | Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity |
| title_fullStr | Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity |
| title_full_unstemmed | Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity |
| title_short | Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity |
| title_sort | single cell rna seq reveals hypothalamic cell diversity |
| topic | single-cell RNA-seq hypothalamus cell heterogeneity oligodendrocyte tanycyte neuron diversity |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717303212 |
| work_keys_str_mv | AT renchaochen singlecellrnaseqrevealshypothalamiccelldiversity AT xiaojiwu singlecellrnaseqrevealshypothalamiccelldiversity AT lanjiang singlecellrnaseqrevealshypothalamiccelldiversity AT yizhang singlecellrnaseqrevealshypothalamiccelldiversity |