Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South Ethiopia
Abstract Background Hospitals provide a reservoir of microorganisms, many of which are multi-resistant to antibiotics. Emergence of multi-drug resistant strains in a hospital environment, particularly in developing countries is an increasing problem to infection treatment. This study aims at assessi...
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| Format: | Article |
| Language: | English |
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BMC
2017-04-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12941-017-0204-2 |
| _version_ | 1818021541041930240 |
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| author | Fithamlak Bisetegen Solomon Fiseha Wada Wadilo Amsalu Amache Arota Yishak Leka Abraham |
| author_facet | Fithamlak Bisetegen Solomon Fiseha Wada Wadilo Amsalu Amache Arota Yishak Leka Abraham |
| author_sort | Fithamlak Bisetegen Solomon |
| collection | DOAJ |
| description | Abstract Background Hospitals provide a reservoir of microorganisms, many of which are multi-resistant to antibiotics. Emergence of multi-drug resistant strains in a hospital environment, particularly in developing countries is an increasing problem to infection treatment. This study aims at assessing antibiotic resistant airborne bacterial isolates. Methods A cross-sectional study was conducted at Wolaita Sodo university teaching and referral Hospital. Indoor air samples were collected by using passive air sampling method. Sample processing and antimicrobial susceptibility testing were done following standard bacteriological techniques. The data was analyzed using SPSS version 20. Results Medically important bacterial pathogens, Coagulase negative staphylococci (29.6%), Staphylococcus aureus (26.3%), Enterococci species, Enterococcus faecalis and Enterococcus faecium (16.5%), Acinetobacter species (9.5%), Escherichia coli (5.8%) and Pseudomonas aeruginosa (5.3%) were isolated. Antibiotic resistance rate ranging from 7.5 to 87.5% was detected for all isolates. Acinetobacter species showed a high rate of resistance for trimethoprim-sulfamethoxazole, gentamicin (78.2%) and ciprofloxacin (82.6%), 28 (38.9%) of S. aureus isolates were meticillin resistant, and 7.5% Enterococci isolates of were vancomycin resistant. 75.3% of all bacterial pathogen were multi-drug resistant. Among them, 74.6% were gram positive and 84% were gram negative. Multi-drug resistance were observed among 84.6% of P. aeruginosa, of 82.5% Enterococcii, E. coli 78.6%, S. aureus 76.6%, and Coagulase negative staphylococci of 73.6%. Conclusions Indoor environment of the hospital was contaminated with airborne microbiotas, which are common cause of post-surgical site infection in the study area. Bacterial isolates were highly resistant to commonly used antibiotics with high multi-drug resistance percentage. So air quality of hospital environment, in restricted settings deserves attention, and requires long-term surveillance to protect both patients and healthcare workers. |
| first_indexed | 2024-04-14T08:20:20Z |
| format | Article |
| id | doaj.art-b24354d717134cdc8d17b700d9a11de8 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-04-14T08:20:20Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-b24354d717134cdc8d17b700d9a11de82022-12-22T02:04:15ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112017-04-011611710.1186/s12941-017-0204-2Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South EthiopiaFithamlak Bisetegen Solomon0Fiseha Wada Wadilo1Amsalu Amache Arota2Yishak Leka Abraham3School of Medicine, College of Health Sciences, Wolaita Sodo UniversitySchool of Medicine, College of Health Sciences, Wolaita Sodo UniversitySchool of Medicine, College of Health Sciences, Wolaita Sodo UniversitySchool of Medicine, College of Health Sciences, Wolaita Sodo UniversityAbstract Background Hospitals provide a reservoir of microorganisms, many of which are multi-resistant to antibiotics. Emergence of multi-drug resistant strains in a hospital environment, particularly in developing countries is an increasing problem to infection treatment. This study aims at assessing antibiotic resistant airborne bacterial isolates. Methods A cross-sectional study was conducted at Wolaita Sodo university teaching and referral Hospital. Indoor air samples were collected by using passive air sampling method. Sample processing and antimicrobial susceptibility testing were done following standard bacteriological techniques. The data was analyzed using SPSS version 20. Results Medically important bacterial pathogens, Coagulase negative staphylococci (29.6%), Staphylococcus aureus (26.3%), Enterococci species, Enterococcus faecalis and Enterococcus faecium (16.5%), Acinetobacter species (9.5%), Escherichia coli (5.8%) and Pseudomonas aeruginosa (5.3%) were isolated. Antibiotic resistance rate ranging from 7.5 to 87.5% was detected for all isolates. Acinetobacter species showed a high rate of resistance for trimethoprim-sulfamethoxazole, gentamicin (78.2%) and ciprofloxacin (82.6%), 28 (38.9%) of S. aureus isolates were meticillin resistant, and 7.5% Enterococci isolates of were vancomycin resistant. 75.3% of all bacterial pathogen were multi-drug resistant. Among them, 74.6% were gram positive and 84% were gram negative. Multi-drug resistance were observed among 84.6% of P. aeruginosa, of 82.5% Enterococcii, E. coli 78.6%, S. aureus 76.6%, and Coagulase negative staphylococci of 73.6%. Conclusions Indoor environment of the hospital was contaminated with airborne microbiotas, which are common cause of post-surgical site infection in the study area. Bacterial isolates were highly resistant to commonly used antibiotics with high multi-drug resistance percentage. So air quality of hospital environment, in restricted settings deserves attention, and requires long-term surveillance to protect both patients and healthcare workers.http://link.springer.com/article/10.1186/s12941-017-0204-2AirborneBacteriaAntibioticResistanceMulti-drug resistance |
| spellingShingle | Fithamlak Bisetegen Solomon Fiseha Wada Wadilo Amsalu Amache Arota Yishak Leka Abraham Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South Ethiopia Annals of Clinical Microbiology and Antimicrobials Airborne Bacteria Antibiotic Resistance Multi-drug resistance |
| title | Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South Ethiopia |
| title_full | Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South Ethiopia |
| title_fullStr | Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South Ethiopia |
| title_full_unstemmed | Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South Ethiopia |
| title_short | Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South Ethiopia |
| title_sort | antibiotic resistant airborne bacteria and their multidrug resistance pattern at university teaching referral hospital in south ethiopia |
| topic | Airborne Bacteria Antibiotic Resistance Multi-drug resistance |
| url | http://link.springer.com/article/10.1186/s12941-017-0204-2 |
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