Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste
This experiment utilised cassava (Manihot utillissima) and arrowroot (Maranta arunadinacea) wastes as the medium of propagation of Saccharomyces cerevisiae to produce β-glucan. The amyloglucosidase hydrolysed the waste, followed by fermentation in the nitrogenous medium by S. cerevisiae. The β-gluca...
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| Format: | Article |
| Language: | English |
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indonesian research institute for biotechnology and bioindustry
2023-04-01
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| Series: | Menara Perkebunan |
| Subjects: | |
| Online Access: | http://mp.iribb.org/mpjurnal/article/view/508 |
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| author | Misri A Gozan Fita Sefriana Yemirta Muhammad Arif Darmawan |
| author_facet | Misri A Gozan Fita Sefriana Yemirta Muhammad Arif Darmawan |
| author_sort | Misri A Gozan |
| collection | DOAJ |
| description | This experiment utilised cassava (Manihot utillissima) and arrowroot (Maranta arunadinacea) wastes as the medium of propagation of Saccharomyces cerevisiae to produce β-glucan. The amyloglucosidase hydrolysed the waste, followed by fermentation in the nitrogenous medium by S. cerevisiae. The β-glucan pellet was extracted using 2% NaOH alkaline solution at 90°C for 5 hours, followed by a series of centrifugation processes. The highest glucose concentration from hydrolysis resulted from adding 57.5 mg amyloglucosidase enzyme for arrowroot waste with 95.93% conversion and 50 mg enzyme for cassava waste with 64.70% conversion. The highest amount was obtained for producing S. cerevisiae by adding 4.75 g peptone to all samples. The optimum number of cells was obtained at 1.61 x 108 colonies at t = 48 hours for arrowroot waste and 8.55 x 107 colonies at t = 48 hours for cassava waste. For β-glucan production, the highest number was obtained by using 3.99 g of peptone for cassava waste with a yield of 1.20% and by using 4.75 g of peptone for arrowroot waste with a yield of 1.23%. For β-glucan pellet, the highest number was 1.77 g L-1 (0.18 % b/v) from cassava waste medium and 1.91 g L-1 (0.19% b/v) from arrowroot waste. Mutant cells in the Yeast Extract–Peptone–Glycerol (YPG) medium produced 6.56 g L-1 (0.66% b/v) β-glucan pellet, while wild-type cells in the similar medium produced 1.84 g L-1 (0.18% b/v). |
| first_indexed | 2024-04-09T14:52:21Z |
| format | Article |
| id | doaj.art-b2463438f6b640b1a96e8963b4d3189f |
| institution | Directory Open Access Journal |
| issn | 0125-9318 1858-3768 |
| language | English |
| last_indexed | 2024-04-09T14:52:21Z |
| publishDate | 2023-04-01 |
| publisher | indonesian research institute for biotechnology and bioindustry |
| record_format | Article |
| series | Menara Perkebunan |
| spelling | doaj.art-b2463438f6b640b1a96e8963b4d3189f2023-05-02T09:18:40Zengindonesian research institute for biotechnology and bioindustryMenara Perkebunan0125-93181858-37682023-04-019111424https://doi.org/10.22302/iribb.jur.mp.v91i1.508Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste Misri A Gozan0Fita Sefriana1Yemirta2Muhammad Arif Darmawan3Bioprocess Engineering Program - Department of Chemical Engineering Faculty of Engineering - Universitas IndonesiaBalai Besar Standardisasi dan Pelayanan Jasa Industri Kimia, Farmasi dan Kemasan, Industrial Department.Balai Besar Standardisasi dan Pelayanan Jasa Industri Kimia, Farmasi dan Kemasan, Industrial Department.Pusat Riset Teknologi Industri Proses dan Manufaktur, BRINThis experiment utilised cassava (Manihot utillissima) and arrowroot (Maranta arunadinacea) wastes as the medium of propagation of Saccharomyces cerevisiae to produce β-glucan. The amyloglucosidase hydrolysed the waste, followed by fermentation in the nitrogenous medium by S. cerevisiae. The β-glucan pellet was extracted using 2% NaOH alkaline solution at 90°C for 5 hours, followed by a series of centrifugation processes. The highest glucose concentration from hydrolysis resulted from adding 57.5 mg amyloglucosidase enzyme for arrowroot waste with 95.93% conversion and 50 mg enzyme for cassava waste with 64.70% conversion. The highest amount was obtained for producing S. cerevisiae by adding 4.75 g peptone to all samples. The optimum number of cells was obtained at 1.61 x 108 colonies at t = 48 hours for arrowroot waste and 8.55 x 107 colonies at t = 48 hours for cassava waste. For β-glucan production, the highest number was obtained by using 3.99 g of peptone for cassava waste with a yield of 1.20% and by using 4.75 g of peptone for arrowroot waste with a yield of 1.23%. For β-glucan pellet, the highest number was 1.77 g L-1 (0.18 % b/v) from cassava waste medium and 1.91 g L-1 (0.19% b/v) from arrowroot waste. Mutant cells in the Yeast Extract–Peptone–Glycerol (YPG) medium produced 6.56 g L-1 (0.66% b/v) β-glucan pellet, while wild-type cells in the similar medium produced 1.84 g L-1 (0.18% b/v).http://mp.iribb.org/mpjurnal/article/view/508amyloglucosidasearrowrootwastecassavawastepellet |
| spellingShingle | Misri A Gozan Fita Sefriana Yemirta Muhammad Arif Darmawan Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste Menara Perkebunan amyloglucosidase arrowrootwaste cassavawaste pellet |
| title | Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste |
| title_full | Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste |
| title_fullStr | Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste |
| title_full_unstemmed | Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste |
| title_short | Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste |
| title_sort | effect of enzymatic hydrolysis and nitrogen on saccharomyces cerevisiae β glucan production from manihot utilissima and maranta arunadinacea waste |
| topic | amyloglucosidase arrowrootwaste cassavawaste pellet |
| url | http://mp.iribb.org/mpjurnal/article/view/508 |
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