Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-Markers
The Dagestan Mountain (DM) cattle breed was established to provide cheese and milk products to mountain dwellers in the specific conditions of the Republic of Dagestan in Southern Russia. Only 650 head of DM cattle were registered in 2020, and their “breed purity” is questionable. We aimed to assess...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/14/7/569 |
| _version_ | 1797406792208089088 |
|---|---|
| author | Valeria V. Volkova Alexandra S. Abdelmanova Tatiana E. Deniskova Olga S. Romanenkova Abdusalam A. Khozhokov Alimsoltan A. Ozdemirov Alexander A. Sermyagin Natalia A. Zinovieva |
| author_facet | Valeria V. Volkova Alexandra S. Abdelmanova Tatiana E. Deniskova Olga S. Romanenkova Abdusalam A. Khozhokov Alimsoltan A. Ozdemirov Alexander A. Sermyagin Natalia A. Zinovieva |
| author_sort | Valeria V. Volkova |
| collection | DOAJ |
| description | The Dagestan Mountain (DM) cattle breed was established to provide cheese and milk products to mountain dwellers in the specific conditions of the Republic of Dagestan in Southern Russia. Only 650 head of DM cattle were registered in 2020, and their “breed purity” is questionable. We aimed to assess the genetic diversity and population structure of modern DM cattle using short tandem repeat (STR) markers. The sample included 150 animals collected from private owners in Dagestan during a scientific expedition (<i>n</i> = 32) and provided by a gene pool farm (<i>n</i> = 118). An additional 166 samples from other cattle breeds distributed in the breeding zone of DM cattle were used as the comparison groups. The genotypes for the 11 STR loci recommended by ISAG were obtained using a genetic analyzer. We identified a high level of genetic diversity as revealed by allelic richness calculations (Ar = 6.82 vs. 4.38–5.82 in other cattle breeds) and observed heterozygosity indices (uHe = 0.76 vs. 0.65–0.72). Based on the STRUCTURE results, animals with low levels of admixture with other breeds were found within the DM cattle, which can be considered as candidates for use in germplasm conservation programs. |
| first_indexed | 2024-03-09T03:30:47Z |
| format | Article |
| id | doaj.art-b246a19d898e41d195878dc364d52d76 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T03:30:47Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-b246a19d898e41d195878dc364d52d762023-12-03T14:55:43ZengMDPI AGDiversity1424-28182022-07-0114756910.3390/d14070569Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-MarkersValeria V. Volkova0Alexandra S. Abdelmanova1Tatiana E. Deniskova2Olga S. Romanenkova3Abdusalam A. Khozhokov4Alimsoltan A. Ozdemirov5Alexander A. Sermyagin6Natalia A. Zinovieva7L.K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, Moscow Region, RussiaL.K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, Moscow Region, RussiaL.K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, Moscow Region, RussiaL.K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, Moscow Region, RussiaFederal State Budgetary Scientific Institution “Federal Agrarian Scientific Center of the Republic of Dagestan”, Akushinsky Ave., Scientific Town, 367014 Makhachkala, Republic of Dagestan, RussiaFederal State Budgetary Scientific Institution “Federal Agrarian Scientific Center of the Republic of Dagestan”, Akushinsky Ave., Scientific Town, 367014 Makhachkala, Republic of Dagestan, RussiaL.K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, Moscow Region, RussiaL.K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, Moscow Region, RussiaThe Dagestan Mountain (DM) cattle breed was established to provide cheese and milk products to mountain dwellers in the specific conditions of the Republic of Dagestan in Southern Russia. Only 650 head of DM cattle were registered in 2020, and their “breed purity” is questionable. We aimed to assess the genetic diversity and population structure of modern DM cattle using short tandem repeat (STR) markers. The sample included 150 animals collected from private owners in Dagestan during a scientific expedition (<i>n</i> = 32) and provided by a gene pool farm (<i>n</i> = 118). An additional 166 samples from other cattle breeds distributed in the breeding zone of DM cattle were used as the comparison groups. The genotypes for the 11 STR loci recommended by ISAG were obtained using a genetic analyzer. We identified a high level of genetic diversity as revealed by allelic richness calculations (Ar = 6.82 vs. 4.38–5.82 in other cattle breeds) and observed heterozygosity indices (uHe = 0.76 vs. 0.65–0.72). Based on the STRUCTURE results, animals with low levels of admixture with other breeds were found within the DM cattle, which can be considered as candidates for use in germplasm conservation programs.https://www.mdpi.com/1424-2818/14/7/569cattlelocal breedsSTR-markerspopulation structure |
| spellingShingle | Valeria V. Volkova Alexandra S. Abdelmanova Tatiana E. Deniskova Olga S. Romanenkova Abdusalam A. Khozhokov Alimsoltan A. Ozdemirov Alexander A. Sermyagin Natalia A. Zinovieva Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-Markers Diversity cattle local breeds STR-markers population structure |
| title | Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-Markers |
| title_full | Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-Markers |
| title_fullStr | Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-Markers |
| title_full_unstemmed | Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-Markers |
| title_short | Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-Markers |
| title_sort | investigation of the genetic diversity of dagestan mountain cattle using str markers |
| topic | cattle local breeds STR-markers population structure |
| url | https://www.mdpi.com/1424-2818/14/7/569 |
| work_keys_str_mv | AT valeriavvolkova investigationofthegeneticdiversityofdagestanmountaincattleusingstrmarkers AT alexandrasabdelmanova investigationofthegeneticdiversityofdagestanmountaincattleusingstrmarkers AT tatianaedeniskova investigationofthegeneticdiversityofdagestanmountaincattleusingstrmarkers AT olgasromanenkova investigationofthegeneticdiversityofdagestanmountaincattleusingstrmarkers AT abdusalamakhozhokov investigationofthegeneticdiversityofdagestanmountaincattleusingstrmarkers AT alimsoltanaozdemirov investigationofthegeneticdiversityofdagestanmountaincattleusingstrmarkers AT alexanderasermyagin investigationofthegeneticdiversityofdagestanmountaincattleusingstrmarkers AT nataliaazinovieva investigationofthegeneticdiversityofdagestanmountaincattleusingstrmarkers |