Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate
Mechanical properties of neuronal cells have a key role for growth, generation of traction forces, adhesion, migration, etc. Mechanical properties are regulated by chemical signaling, neurotransmitters, and neuronal ion exchange. Disturbance of chemical signaling is accompanied by several diseases s...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/12/20/2428 |
| _version_ | 1827721393509236736 |
|---|---|
| author | Vasilii Kolmogorov Alexander Erofeev Alexander Vaneev Lyubov Gorbacheva Dmitry Kolesov Natalia Klyachko Yuri Korchev Petr Gorelkin |
| author_facet | Vasilii Kolmogorov Alexander Erofeev Alexander Vaneev Lyubov Gorbacheva Dmitry Kolesov Natalia Klyachko Yuri Korchev Petr Gorelkin |
| author_sort | Vasilii Kolmogorov |
| collection | DOAJ |
| description | Mechanical properties of neuronal cells have a key role for growth, generation of traction forces, adhesion, migration, etc. Mechanical properties are regulated by chemical signaling, neurotransmitters, and neuronal ion exchange. Disturbance of chemical signaling is accompanied by several diseases such as ischemia, trauma, and neurodegenerative diseases. It is known that the disturbance of chemical signaling, like that caused by glutamate excitotoxicity, leads to the structural reorganization of the cytoskeleton of neuronal cells and the deviation of native mechanical properties. Thus, to investigate the mechanical properties of living neuronal cells in the presence of glutamate, it is crucial to use noncontact and low-stress methods, which are the advantages of scanning ion-conductance microscopy (SICM). Moreover, a nanopipette may be used for the local delivery of small molecules as well as for a probe. In this work, SICM was used as an advanced technique for the simultaneous local delivery of glutamate and investigation of living neuronal cell morphology and mechanical behavior caused by an excitotoxic effect of glutamate. |
| first_indexed | 2024-03-10T21:21:51Z |
| format | Article |
| id | doaj.art-b246baa92fa3470d91614061ec628f5f |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T21:21:51Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b246baa92fa3470d91614061ec628f5f2023-11-19T16:02:00ZengMDPI AGCells2073-44092023-10-011220242810.3390/cells12202428Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of GlutamateVasilii Kolmogorov0Alexander Erofeev1Alexander Vaneev2Lyubov Gorbacheva3Dmitry Kolesov4Natalia Klyachko5Yuri Korchev6Petr Gorelkin7Research Laboratory of Biophysics, National University of Science and Technology “MISIS”, Moscow 119049, RussiaResearch Laboratory of Biophysics, National University of Science and Technology “MISIS”, Moscow 119049, RussiaResearch Laboratory of Biophysics, National University of Science and Technology “MISIS”, Moscow 119049, RussiaFaculty of Biology, Lomonosov Moscow State University, Moscow 119991, RussiaResearch Laboratory of SPM, Moscow Polytechnic University, Moscow 107023, RussiaFaculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, RussiaDepartment of Medicine, Imperial College London, London SW7 2BX, UKResearch Laboratory of Biophysics, National University of Science and Technology “MISIS”, Moscow 119049, RussiaMechanical properties of neuronal cells have a key role for growth, generation of traction forces, adhesion, migration, etc. Mechanical properties are regulated by chemical signaling, neurotransmitters, and neuronal ion exchange. Disturbance of chemical signaling is accompanied by several diseases such as ischemia, trauma, and neurodegenerative diseases. It is known that the disturbance of chemical signaling, like that caused by glutamate excitotoxicity, leads to the structural reorganization of the cytoskeleton of neuronal cells and the deviation of native mechanical properties. Thus, to investigate the mechanical properties of living neuronal cells in the presence of glutamate, it is crucial to use noncontact and low-stress methods, which are the advantages of scanning ion-conductance microscopy (SICM). Moreover, a nanopipette may be used for the local delivery of small molecules as well as for a probe. In this work, SICM was used as an advanced technique for the simultaneous local delivery of glutamate and investigation of living neuronal cell morphology and mechanical behavior caused by an excitotoxic effect of glutamate.https://www.mdpi.com/2073-4409/12/20/2428scanning probe microscopyscanning ion-conductance microscopycell biomechanicsnanopipettelocal deliveryneuronal cells |
| spellingShingle | Vasilii Kolmogorov Alexander Erofeev Alexander Vaneev Lyubov Gorbacheva Dmitry Kolesov Natalia Klyachko Yuri Korchev Petr Gorelkin Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate Cells scanning probe microscopy scanning ion-conductance microscopy cell biomechanics nanopipette local delivery neuronal cells |
| title | Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate |
| title_full | Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate |
| title_fullStr | Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate |
| title_full_unstemmed | Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate |
| title_short | Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate |
| title_sort | scanning ion conductance microscopy for studying mechanical properties of neuronal cells during local delivery of glutamate |
| topic | scanning probe microscopy scanning ion-conductance microscopy cell biomechanics nanopipette local delivery neuronal cells |
| url | https://www.mdpi.com/2073-4409/12/20/2428 |
| work_keys_str_mv | AT vasiliikolmogorov scanningionconductancemicroscopyforstudyingmechanicalpropertiesofneuronalcellsduringlocaldeliveryofglutamate AT alexandererofeev scanningionconductancemicroscopyforstudyingmechanicalpropertiesofneuronalcellsduringlocaldeliveryofglutamate AT alexandervaneev scanningionconductancemicroscopyforstudyingmechanicalpropertiesofneuronalcellsduringlocaldeliveryofglutamate AT lyubovgorbacheva scanningionconductancemicroscopyforstudyingmechanicalpropertiesofneuronalcellsduringlocaldeliveryofglutamate AT dmitrykolesov scanningionconductancemicroscopyforstudyingmechanicalpropertiesofneuronalcellsduringlocaldeliveryofglutamate AT nataliaklyachko scanningionconductancemicroscopyforstudyingmechanicalpropertiesofneuronalcellsduringlocaldeliveryofglutamate AT yurikorchev scanningionconductancemicroscopyforstudyingmechanicalpropertiesofneuronalcellsduringlocaldeliveryofglutamate AT petrgorelkin scanningionconductancemicroscopyforstudyingmechanicalpropertiesofneuronalcellsduringlocaldeliveryofglutamate |