Discovery of a novel nucleoside immune signaling molecule 2′-deoxyguanosine in microbes and plants
Introduction: Beneficial microorganisms play essential roles in plant growth and induced systemic resistance (ISR) by releasing signaling molecules. Our previous study obtained the crude extract from beneficial endophyte Paecilomyces variotii, termed ZNC (ZhiNengCong), which significantly enhanced p...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-04-01
|
| Series: | Journal of Advanced Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123222001539 |
| _version_ | 1797854552933793792 |
|---|---|
| author | Chongchong Lu Qingbin Wang Yanke Jiang Min Zhang Xuanlin Meng Yang Li Baoyou Liu Ziyi Yin Haifeng Liu Chune Peng Fuchuan Li Yingzhe Yue Mingxia Hao Yurong Sui Lulu Wang Guodong Cheng Jianzhu Liu Zhaohui Chu Changxiang Zhu Hansong Dong Xinhua Ding |
| author_facet | Chongchong Lu Qingbin Wang Yanke Jiang Min Zhang Xuanlin Meng Yang Li Baoyou Liu Ziyi Yin Haifeng Liu Chune Peng Fuchuan Li Yingzhe Yue Mingxia Hao Yurong Sui Lulu Wang Guodong Cheng Jianzhu Liu Zhaohui Chu Changxiang Zhu Hansong Dong Xinhua Ding |
| author_sort | Chongchong Lu |
| collection | DOAJ |
| description | Introduction: Beneficial microorganisms play essential roles in plant growth and induced systemic resistance (ISR) by releasing signaling molecules. Our previous study obtained the crude extract from beneficial endophyte Paecilomyces variotii, termed ZNC (ZhiNengCong), which significantly enhanced plant resistance to pathogen even at 100 ng/ml. However, the immunoreactive components of ZNC remain unclear. Here, we further identified one of the immunoreactive components of ZNC is a nucleoside 2′-deoxyguanosine (2-dG). Objectives: This paper intends to reveal the molecular mechanism of microbial-derived 2′-deoxyguanosine (2-dG) in activating plant immunity, and the role of plant-derived 2-dG in plant immunity. Methods: The components of ZNC were separated using a high-performance liquid chromatography (HPLC), and 2-dG is identified using a HPLC–mass spectrometry system (LC-MS). Transcriptome analysis and genetic experiments were used to reveal the immune signaling pathway dependent on 2-dG activation of plant immunity. Results: This study identified 2′-deoxyguanosine (2-dG) as one of the immunoreactive components from ZNC. And 2-dG significantly enhanced plant pathogen resistance even at 10 ng/ml (37.42 nM). Furthermore, 2-dG-induced resistance depends on NPR1, pattern-recognition receptors/coreceptors, ATP receptor P2K1 (DORN1), ethylene signaling but not salicylic acid accumulation. In addition, we identified Arabidopsis VENOSA4 (VEN4) was involved in 2-dG biosynthesis and could convert dGTP to 2-dG, and vne4 mutant plants were more susceptible to pathogens. Conclusion: In summary, microbial-derived 2-dG may act as a novel immune signaling molecule involved in plant-microorganism interactions, and VEN4 is 2-dG biosynthesis gene and plays a key role in plant immunity. |
| first_indexed | 2024-04-09T20:09:02Z |
| format | Article |
| id | doaj.art-b249b720f54d4faea8f6d51de0840238 |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-04-09T20:09:02Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-b249b720f54d4faea8f6d51de08402382023-04-01T08:47:10ZengElsevierJournal of Advanced Research2090-12322023-04-0146115Discovery of a novel nucleoside immune signaling molecule 2′-deoxyguanosine in microbes and plantsChongchong Lu0Qingbin Wang1Yanke Jiang2Min Zhang3Xuanlin Meng4Yang Li5Baoyou Liu6Ziyi Yin7Haifeng Liu8Chune Peng9Fuchuan Li10Yingzhe Yue11Mingxia Hao12Yurong Sui13Lulu Wang14Guodong Cheng15Jianzhu Liu16Zhaohui Chu17Changxiang Zhu18Hansong Dong19Xinhua Ding20State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaShandong Pengbo Biotechnology Co., LTD, Taian 271018, China; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaNational Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 72 Binhai Rd, Qingdao 266200, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Hybrid Rice, Hubei Hongshan Laboratory, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271018, China; Corresponding author.Introduction: Beneficial microorganisms play essential roles in plant growth and induced systemic resistance (ISR) by releasing signaling molecules. Our previous study obtained the crude extract from beneficial endophyte Paecilomyces variotii, termed ZNC (ZhiNengCong), which significantly enhanced plant resistance to pathogen even at 100 ng/ml. However, the immunoreactive components of ZNC remain unclear. Here, we further identified one of the immunoreactive components of ZNC is a nucleoside 2′-deoxyguanosine (2-dG). Objectives: This paper intends to reveal the molecular mechanism of microbial-derived 2′-deoxyguanosine (2-dG) in activating plant immunity, and the role of plant-derived 2-dG in plant immunity. Methods: The components of ZNC were separated using a high-performance liquid chromatography (HPLC), and 2-dG is identified using a HPLC–mass spectrometry system (LC-MS). Transcriptome analysis and genetic experiments were used to reveal the immune signaling pathway dependent on 2-dG activation of plant immunity. Results: This study identified 2′-deoxyguanosine (2-dG) as one of the immunoreactive components from ZNC. And 2-dG significantly enhanced plant pathogen resistance even at 10 ng/ml (37.42 nM). Furthermore, 2-dG-induced resistance depends on NPR1, pattern-recognition receptors/coreceptors, ATP receptor P2K1 (DORN1), ethylene signaling but not salicylic acid accumulation. In addition, we identified Arabidopsis VENOSA4 (VEN4) was involved in 2-dG biosynthesis and could convert dGTP to 2-dG, and vne4 mutant plants were more susceptible to pathogens. Conclusion: In summary, microbial-derived 2-dG may act as a novel immune signaling molecule involved in plant-microorganism interactions, and VEN4 is 2-dG biosynthesis gene and plays a key role in plant immunity.http://www.sciencedirect.com/science/article/pii/S2090123222001539Endophytic fungiPlant immune elicitorET signalingNPR1Pattern- recognition receptors/coreceptors |
| spellingShingle | Chongchong Lu Qingbin Wang Yanke Jiang Min Zhang Xuanlin Meng Yang Li Baoyou Liu Ziyi Yin Haifeng Liu Chune Peng Fuchuan Li Yingzhe Yue Mingxia Hao Yurong Sui Lulu Wang Guodong Cheng Jianzhu Liu Zhaohui Chu Changxiang Zhu Hansong Dong Xinhua Ding Discovery of a novel nucleoside immune signaling molecule 2′-deoxyguanosine in microbes and plants Journal of Advanced Research Endophytic fungi Plant immune elicitor ET signaling NPR1 Pattern- recognition receptors/coreceptors |
| title | Discovery of a novel nucleoside immune signaling molecule 2′-deoxyguanosine in microbes and plants |
| title_full | Discovery of a novel nucleoside immune signaling molecule 2′-deoxyguanosine in microbes and plants |
| title_fullStr | Discovery of a novel nucleoside immune signaling molecule 2′-deoxyguanosine in microbes and plants |
| title_full_unstemmed | Discovery of a novel nucleoside immune signaling molecule 2′-deoxyguanosine in microbes and plants |
| title_short | Discovery of a novel nucleoside immune signaling molecule 2′-deoxyguanosine in microbes and plants |
| title_sort | discovery of a novel nucleoside immune signaling molecule 2 deoxyguanosine in microbes and plants |
| topic | Endophytic fungi Plant immune elicitor ET signaling NPR1 Pattern- recognition receptors/coreceptors |
| url | http://www.sciencedirect.com/science/article/pii/S2090123222001539 |
| work_keys_str_mv | AT chongchonglu discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT qingbinwang discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT yankejiang discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT minzhang discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT xuanlinmeng discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT yangli discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT baoyouliu discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT ziyiyin discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT haifengliu discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT chunepeng discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT fuchuanli discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT yingzheyue discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT mingxiahao discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT yurongsui discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT luluwang discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT guodongcheng discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT jianzhuliu discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT zhaohuichu discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT changxiangzhu discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT hansongdong discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants AT xinhuading discoveryofanovelnucleosideimmunesignalingmolecule2deoxyguanosineinmicrobesandplants |