Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease
Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the digestive tract and is closely associated with the homeostasis of the gut microbiota. Inulin, as a natural prebiotic, displays anti-inflammatory activity and maintains equilibrium of the intestinal microbiota. In this study,...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yao Du Kanta Kusama Koki Hama Xinyue Chen Yu Tahara Susumu Kajiwara Shigenobu Shibata Kanami Orihara |
| author_facet | Yao Du Kanta Kusama Koki Hama Xinyue Chen Yu Tahara Susumu Kajiwara Shigenobu Shibata Kanami Orihara |
| author_sort | Yao Du |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the digestive tract and is closely associated with the homeostasis of the gut microbiota. Inulin, as a natural prebiotic, displays anti-inflammatory activity and maintains equilibrium of the intestinal microbiota. In this study, our research aimed to explore the potential of inulin in enhancing intestinal immunity and reducing inflammation in stress-recurrent IBD. In this study, a co-culture intestinal epithelium model and a stress-recurrent IBD mouse model was used to examine the protective effects of inulin. It was observed that inulin digesta significantly reduced pro-inflammatory cytokine expression (<i>CXCL8/IL8</i> and <i>TNFA</i>) and increased <i>MUC2</i> expression in intestinal epithelial cells. In vivo, our findings showed that Inulin intake significantly prevented IBD symptoms. This was substantiated by a decrease in serum inflammatory markers (IL-6, CALP) and a downregulation of inflammatory cytokine (<i>Il6</i>) in colon samples. Additionally, inulin intake led to an increase in short-chain fatty acids (SCFAs) in cecal contents and a reduction in the expression of endoplasmic reticulum (ER) stress markers (CHOP, BiP). Our results highlight that inulin can improve stress-recurrent IBD symptoms by modulating microbiota composition, reducing inflammation, and alleviating ER stress. These findings suggested the therapeutic potential of inulin as a dietary intervention for ameliorating stress-recurrent IBD. |
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| id | doaj.art-b24a627c9ec748c8874b5dbf4d8e0349 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:29:17Z |
| publishDate | 2024-02-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b24a627c9ec748c8874b5dbf4d8e03492024-03-12T16:45:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01255249410.3390/ijms25052494Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel DiseaseYao Du0Kanta Kusama1Koki Hama2Xinyue Chen3Yu Tahara4Susumu Kajiwara5Shigenobu Shibata6Kanami Orihara7School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, JapanLaboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo 162-8480, JapanLaboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo 162-8480, JapanSchool of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, JapanLaboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo 162-8480, JapanSchool of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, JapanLaboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo 162-8480, JapanSchool of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, JapanInflammatory bowel disease (IBD) is a chronic inflammatory condition of the digestive tract and is closely associated with the homeostasis of the gut microbiota. Inulin, as a natural prebiotic, displays anti-inflammatory activity and maintains equilibrium of the intestinal microbiota. In this study, our research aimed to explore the potential of inulin in enhancing intestinal immunity and reducing inflammation in stress-recurrent IBD. In this study, a co-culture intestinal epithelium model and a stress-recurrent IBD mouse model was used to examine the protective effects of inulin. It was observed that inulin digesta significantly reduced pro-inflammatory cytokine expression (<i>CXCL8/IL8</i> and <i>TNFA</i>) and increased <i>MUC2</i> expression in intestinal epithelial cells. In vivo, our findings showed that Inulin intake significantly prevented IBD symptoms. This was substantiated by a decrease in serum inflammatory markers (IL-6, CALP) and a downregulation of inflammatory cytokine (<i>Il6</i>) in colon samples. Additionally, inulin intake led to an increase in short-chain fatty acids (SCFAs) in cecal contents and a reduction in the expression of endoplasmic reticulum (ER) stress markers (CHOP, BiP). Our results highlight that inulin can improve stress-recurrent IBD symptoms by modulating microbiota composition, reducing inflammation, and alleviating ER stress. These findings suggested the therapeutic potential of inulin as a dietary intervention for ameliorating stress-recurrent IBD.https://www.mdpi.com/1422-0067/25/5/2494inulinIBDstressSCFAscytokinesmucin |
| spellingShingle | Yao Du Kanta Kusama Koki Hama Xinyue Chen Yu Tahara Susumu Kajiwara Shigenobu Shibata Kanami Orihara Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease International Journal of Molecular Sciences inulin IBD stress SCFAs cytokines mucin |
| title | Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease |
| title_full | Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease |
| title_fullStr | Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease |
| title_full_unstemmed | Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease |
| title_short | Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease |
| title_sort | protective effects of inulin on stress recurrent inflammatory bowel disease |
| topic | inulin IBD stress SCFAs cytokines mucin |
| url | https://www.mdpi.com/1422-0067/25/5/2494 |
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