Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal
Abstract Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to l...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14781-y |
| _version_ | 1811340187243905024 |
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| author | Cristin C. W. Young Dibesh Karmacharya Manisha Bista Ajay N. Sharma Tracey Goldstein Jonna A. K. Mazet Christine K. Johnson |
| author_facet | Cristin C. W. Young Dibesh Karmacharya Manisha Bista Ajay N. Sharma Tracey Goldstein Jonna A. K. Mazet Christine K. Johnson |
| author_sort | Cristin C. W. Young |
| collection | DOAJ |
| description | Abstract Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to low- and middle-income countries and in community settings, where an estimated 80% of all antibiotics are used. This study features AMR genes found among humans, animals, and water at an urban informal settlement in Nepal with intensifying livestock production. We sampled humans, chickens, ducks, swine, and water clustered by household, as well as rodents and shrews near dwellings, concurrently in time in July 2017 in southeastern Kathmandu along the Manohara river. Real-time qualitative PCR was performed to screen for 88 genes. Our results characterize the animal-human-environmental interfaces related to the occurrence of specific resistance genes (bla SHV-1 (SHV(238G240E) strain), QnrS, ermC, tetA, tetB, aacC2, aadA1) associated with antibiotics of global health importance that comprise several drug classes, including aminoglycosides, beta-lactams, tetracyclines, macrolides, and fluoroquinolones. By characterizing risk factors across AMR genes of public health importance, this research highlights potential transmission pathways for further investigation and provides prioritization of community-based prevention and intervention efforts for disrupting AMR transmission of critically important antibiotics used in both humans and animals in Nepal. |
| first_indexed | 2024-04-13T18:37:20Z |
| format | Article |
| id | doaj.art-b24b7f69c53648969d20f4a4419b0690 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T18:37:20Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b24b7f69c53648969d20f4a4419b06902022-12-22T02:34:50ZengNature PortfolioScientific Reports2045-23222022-08-0112111310.1038/s41598-022-14781-yAntibiotic resistance genes of public health importance in livestock and humans in an informal urban community in NepalCristin C. W. Young0Dibesh Karmacharya1Manisha Bista2Ajay N. Sharma3Tracey Goldstein4Jonna A. K. Mazet5Christine K. Johnson6EpiCenter for Disease Dynamics, School of Veterinary Medicine, University of California, DavisCenter for Molecular Dynamics, Nepal (CMDN)Mott MacDonaldCenter for Molecular Dynamics, Nepal (CMDN)One Health Institute, School of Veterinary Medicine, University of CaliforniaOne Health Institute, School of Veterinary Medicine, University of CaliforniaEpiCenter for Disease Dynamics, School of Veterinary Medicine, University of California, DavisAbstract Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to low- and middle-income countries and in community settings, where an estimated 80% of all antibiotics are used. This study features AMR genes found among humans, animals, and water at an urban informal settlement in Nepal with intensifying livestock production. We sampled humans, chickens, ducks, swine, and water clustered by household, as well as rodents and shrews near dwellings, concurrently in time in July 2017 in southeastern Kathmandu along the Manohara river. Real-time qualitative PCR was performed to screen for 88 genes. Our results characterize the animal-human-environmental interfaces related to the occurrence of specific resistance genes (bla SHV-1 (SHV(238G240E) strain), QnrS, ermC, tetA, tetB, aacC2, aadA1) associated with antibiotics of global health importance that comprise several drug classes, including aminoglycosides, beta-lactams, tetracyclines, macrolides, and fluoroquinolones. By characterizing risk factors across AMR genes of public health importance, this research highlights potential transmission pathways for further investigation and provides prioritization of community-based prevention and intervention efforts for disrupting AMR transmission of critically important antibiotics used in both humans and animals in Nepal.https://doi.org/10.1038/s41598-022-14781-y |
| spellingShingle | Cristin C. W. Young Dibesh Karmacharya Manisha Bista Ajay N. Sharma Tracey Goldstein Jonna A. K. Mazet Christine K. Johnson Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal Scientific Reports |
| title | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
| title_full | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
| title_fullStr | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
| title_full_unstemmed | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
| title_short | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
| title_sort | antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in nepal |
| url | https://doi.org/10.1038/s41598-022-14781-y |
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