ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER?
ABSTRACT Background: CD133 and AXL have been described as cancer stem cell markers, and c-MYC as a key regulatory cellular mechanism in colorectal cancer (CRC). Aim: Evaluate the prognostic role of the biomarkers CD133, AXL and c-MYC and their association with clinicopathologic characteristics in...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Cirurgia Digestiva
2021-03-01
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| Series: | ABCD: Arquivos Brasileiros de Cirurgia Digestiva |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202020000400310&tlng=en |
| _version_ | 1828126182169640960 |
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| author | Leticia Elizabeth Augustin CZECZKO Carmen Australia Paredes Marcondes RIBAS Nicolau Gregori CZECZKO Thelma Larocca SKARE Camila Kienen YAMAKAWA Guilherme GIONEDIS Cecilia VASCONCELOS Fabiola Pabst BREMER Diogo Francesco CASTOLDI Martin GASSER Ana Maria WAAGA-GASSER |
| author_facet | Leticia Elizabeth Augustin CZECZKO Carmen Australia Paredes Marcondes RIBAS Nicolau Gregori CZECZKO Thelma Larocca SKARE Camila Kienen YAMAKAWA Guilherme GIONEDIS Cecilia VASCONCELOS Fabiola Pabst BREMER Diogo Francesco CASTOLDI Martin GASSER Ana Maria WAAGA-GASSER |
| author_sort | Leticia Elizabeth Augustin CZECZKO |
| collection | DOAJ |
| description | ABSTRACT Background: CD133 and AXL have been described as cancer stem cell markers, and c-MYC as a key regulatory cellular mechanism in colorectal cancer (CRC). Aim: Evaluate the prognostic role of the biomarkers CD133, AXL and c-MYC and their association with clinicopathologic characteristics in colorectal adenocarcinomas and adenomas. Methods: A total of 156 patients with UICC stage I-IV adenocarcinomas (n=122) and adenomas (n=34) were analyzed. Tissue microarrays (TMA) from primary tumors and polyps for CD133, c-MYC and AXL expression were performed and analyzed for their significance with clinicopathologic characteristics. Results: Poorly differentiated adenocarcinomas and disease progression were independent risk factors for poor overall survival. The median overall survival time was 30 months. Positive CD133 expression (35.9% of all cases), particularly of right-sided CRCs (44.8% of the CD133+ cases), was negatively correlated with death in the univariate analysis, which did not reach significance in the multivariate analysis. c-MYC (15.4% of all cases) was predominantly expressed in advanced-stage patients with distant (non-pulmonary/non-hepatic) metastasis. AXL expression was found only occasionally, and predominantly dominated in adenomas, with less penetrance in high-grade dysplasia. Conclusions: CD133 expression was not associated with inferior overall survival in CRC. While AXL showed inconclusive results, c-MYC expression in primary CRCs was associated with distant metastasis. |
| first_indexed | 2024-04-11T15:31:37Z |
| format | Article |
| id | doaj.art-b24c6f4b1f62470ba2bcd6db6578b0cc |
| institution | Directory Open Access Journal |
| issn | 0102-6720 |
| language | English |
| last_indexed | 2024-04-11T15:31:37Z |
| publishDate | 2021-03-01 |
| publisher | Colégio Brasileiro de Cirurgia Digestiva |
| record_format | Article |
| series | ABCD: Arquivos Brasileiros de Cirurgia Digestiva |
| spelling | doaj.art-b24c6f4b1f62470ba2bcd6db6578b0cc2022-12-22T04:16:08ZengColégio Brasileiro de Cirurgia DigestivaABCD: Arquivos Brasileiros de Cirurgia Digestiva0102-67202021-03-0133410.1590/0102-672020200004e1568ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER?Leticia Elizabeth Augustin CZECZKOhttps://orcid.org/0000-0003-4732-9690Carmen Australia Paredes Marcondes RIBAShttps://orcid.org/0000-0002-6195-046XNicolau Gregori CZECZKOhttps://orcid.org/0000-0002-5926-150XThelma Larocca SKAREhttps://orcid.org/0000-0002-7699-3542Camila Kienen YAMAKAWAhttps://orcid.org/0000-0002-6582-2510Guilherme GIONEDIShttps://orcid.org/0000-0002-0768-9408Cecilia VASCONCELOShttps://orcid.org/0000-0002-5108-3163Fabiola Pabst BREMERhttps://orcid.org/0000-0002-6817-8409Diogo Francesco CASTOLDIhttps://orcid.org/0000-0003-1274-6814Martin GASSERhttps://orcid.org/0000-0002-4987-8389Ana Maria WAAGA-GASSERhttps://orcid.org/0000-0002-5587-5256ABSTRACT Background: CD133 and AXL have been described as cancer stem cell markers, and c-MYC as a key regulatory cellular mechanism in colorectal cancer (CRC). Aim: Evaluate the prognostic role of the biomarkers CD133, AXL and c-MYC and their association with clinicopathologic characteristics in colorectal adenocarcinomas and adenomas. Methods: A total of 156 patients with UICC stage I-IV adenocarcinomas (n=122) and adenomas (n=34) were analyzed. Tissue microarrays (TMA) from primary tumors and polyps for CD133, c-MYC and AXL expression were performed and analyzed for their significance with clinicopathologic characteristics. Results: Poorly differentiated adenocarcinomas and disease progression were independent risk factors for poor overall survival. The median overall survival time was 30 months. Positive CD133 expression (35.9% of all cases), particularly of right-sided CRCs (44.8% of the CD133+ cases), was negatively correlated with death in the univariate analysis, which did not reach significance in the multivariate analysis. c-MYC (15.4% of all cases) was predominantly expressed in advanced-stage patients with distant (non-pulmonary/non-hepatic) metastasis. AXL expression was found only occasionally, and predominantly dominated in adenomas, with less penetrance in high-grade dysplasia. Conclusions: CD133 expression was not associated with inferior overall survival in CRC. While AXL showed inconclusive results, c-MYC expression in primary CRCs was associated with distant metastasis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202020000400310&tlng=enneoplasmsAdenomaBiomarkers, tumorProto-oncogene proteins c-MYCAC133 antigenReceptor protein tyrosine-kinase |
| spellingShingle | Leticia Elizabeth Augustin CZECZKO Carmen Australia Paredes Marcondes RIBAS Nicolau Gregori CZECZKO Thelma Larocca SKARE Camila Kienen YAMAKAWA Guilherme GIONEDIS Cecilia VASCONCELOS Fabiola Pabst BREMER Diogo Francesco CASTOLDI Martin GASSER Ana Maria WAAGA-GASSER ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER? ABCD: Arquivos Brasileiros de Cirurgia Digestiva neoplasms Adenoma Biomarkers, tumor Proto-oncogene proteins c-MYC AC133 antigen Receptor protein tyrosine-kinase |
| title | ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER? |
| title_full | ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER? |
| title_fullStr | ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER? |
| title_full_unstemmed | ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER? |
| title_short | ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER? |
| title_sort | are stem cell marker expression and cd133 analysis relevant to differentiate colorectal cancer |
| topic | neoplasms Adenoma Biomarkers, tumor Proto-oncogene proteins c-MYC AC133 antigen Receptor protein tyrosine-kinase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202020000400310&tlng=en |
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