Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon
Acute febrile patients presenting at hospitals in Douala, Cameroon between July and December 2020, were screened for dengue infections using real time RT-PCR on fragments of the 5’ and 3’ UTR genomic regions. In total, 12.8% (41/320) of cases examined were positive for dengue. Dengue virus 3 (DENV-3...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-10-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568189/?tool=EBI |
| _version_ | 1818821123241934848 |
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| author | Huguette Simo Tchetgna Francine Sado Yousseu Basile Kamgang Armel Tedjou Philip J. McCall Charles S. Wondji |
| author_facet | Huguette Simo Tchetgna Francine Sado Yousseu Basile Kamgang Armel Tedjou Philip J. McCall Charles S. Wondji |
| author_sort | Huguette Simo Tchetgna |
| collection | DOAJ |
| description | Acute febrile patients presenting at hospitals in Douala, Cameroon between July and December 2020, were screened for dengue infections using real time RT-PCR on fragments of the 5’ and 3’ UTR genomic regions. In total, 12.8% (41/320) of cases examined were positive for dengue. Dengue virus 3 (DENV-3) was the most common serotype found (68.3%), followed by DENV-2 (19.5%) and DENV-1 (4.9%). Co-infections of DENV-3 and DENV-2 were found in 3 cases. Jaundice and headache were the most frequent clinical signs associated with infection and 56% (23/41) of the cases were co-infections with malaria. Phylogenetic analysis of the envelope gene identified DENV-1 as belonging to genotype V, DENV-2 to genotype II and DENV-3 to genotype III. The simultaneous occurrence of three serotypes in Douala reveals dengue as a serious public health threat for Cameroon and highlights the need for further epidemiological studies in the major cities of this region. Author summary Acute febrile patients presenting at hospitals in Douala, Cameroon between July and December 2020, were screened for dengue infections by Polymerase chain reaction. In total, 12.8% (41/320) of cases examined were infected by dengue virus. Dengue virus 3 (DENV-3) was the most common serotype found (68.3%), followed by DENV-2 (19.5%) and DENV-1 (4.9%). Co-infections of DENV-3 and DENV-2 were found in 3 cases. Jaundice and headache were the most frequent clinical signs associated with infection and 56% (23/41) of the cases were co-infections with malaria. The simultaneous occurrence of three serotypes in Douala reveals dengue as a serious public health threat for Cameroon and highlights the need for further epidemiological studies in the major cities of this region. |
| first_indexed | 2024-12-18T23:03:11Z |
| format | Article |
| id | doaj.art-b24d1ab20fbd41c2aa68defe6a890460 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-18T23:03:11Z |
| publishDate | 2021-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-b24d1ab20fbd41c2aa68defe6a8904602022-12-21T20:48:32ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352021-10-011510Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in CameroonHuguette Simo TchetgnaFrancine Sado YousseuBasile KamgangArmel TedjouPhilip J. McCallCharles S. WondjiAcute febrile patients presenting at hospitals in Douala, Cameroon between July and December 2020, were screened for dengue infections using real time RT-PCR on fragments of the 5’ and 3’ UTR genomic regions. In total, 12.8% (41/320) of cases examined were positive for dengue. Dengue virus 3 (DENV-3) was the most common serotype found (68.3%), followed by DENV-2 (19.5%) and DENV-1 (4.9%). Co-infections of DENV-3 and DENV-2 were found in 3 cases. Jaundice and headache were the most frequent clinical signs associated with infection and 56% (23/41) of the cases were co-infections with malaria. Phylogenetic analysis of the envelope gene identified DENV-1 as belonging to genotype V, DENV-2 to genotype II and DENV-3 to genotype III. The simultaneous occurrence of three serotypes in Douala reveals dengue as a serious public health threat for Cameroon and highlights the need for further epidemiological studies in the major cities of this region. Author summary Acute febrile patients presenting at hospitals in Douala, Cameroon between July and December 2020, were screened for dengue infections by Polymerase chain reaction. In total, 12.8% (41/320) of cases examined were infected by dengue virus. Dengue virus 3 (DENV-3) was the most common serotype found (68.3%), followed by DENV-2 (19.5%) and DENV-1 (4.9%). Co-infections of DENV-3 and DENV-2 were found in 3 cases. Jaundice and headache were the most frequent clinical signs associated with infection and 56% (23/41) of the cases were co-infections with malaria. The simultaneous occurrence of three serotypes in Douala reveals dengue as a serious public health threat for Cameroon and highlights the need for further epidemiological studies in the major cities of this region.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568189/?tool=EBI |
| spellingShingle | Huguette Simo Tchetgna Francine Sado Yousseu Basile Kamgang Armel Tedjou Philip J. McCall Charles S. Wondji Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon PLoS Neglected Tropical Diseases |
| title | Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon |
| title_full | Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon |
| title_fullStr | Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon |
| title_full_unstemmed | Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon |
| title_short | Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon |
| title_sort | concurrent circulation of dengue serotype 1 2 and 3 among acute febrile patients in cameroon |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568189/?tool=EBI |
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