Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies
Abstract Understanding mechanisms and manifestations of cardiovascular risk factors, including diabetes, on vascular cells such as endothelial cells, pericytes, and vascular smooth muscle cells, remains elusive partly due to the lack of appropriate disease models. Therefore, here we explore differen...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-023-03521-2 |
| _version_ | 1797577767417544704 |
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| author | Hojjat Naderi-Meshkin Victoria A. Cornelius Magdalini Eleftheriadou Koray Niels Potel Wiwit Ananda Wahyu Setyaningsih Andriana Margariti |
| author_facet | Hojjat Naderi-Meshkin Victoria A. Cornelius Magdalini Eleftheriadou Koray Niels Potel Wiwit Ananda Wahyu Setyaningsih Andriana Margariti |
| author_sort | Hojjat Naderi-Meshkin |
| collection | DOAJ |
| description | Abstract Understanding mechanisms and manifestations of cardiovascular risk factors, including diabetes, on vascular cells such as endothelial cells, pericytes, and vascular smooth muscle cells, remains elusive partly due to the lack of appropriate disease models. Therefore, here we explore different aspects for the development of advanced 3D in vitro disease models that recapitulate human blood vessel complications using patient-derived induced pluripotent stem cells, which retain the epigenetic, transcriptomic, and metabolic memory of their patient-of-origin. In this review, we highlight the superiority of 3D blood vessel organoids over conventional 2D cell culture systems for vascular research. We outline the key benefits of vascular organoids in both health and disease contexts and discuss the current challenges associated with organoid technology, providing potential solutions. Furthermore, we discuss the diverse applications of vascular organoids and emphasize the importance of incorporating all relevant cellular components in a 3D model to accurately recapitulate vascular pathophysiology. As a specific example, we present a comprehensive overview of diabetic vasculopathy, demonstrating how the interplay of different vascular cell types is critical for the successful modelling of complex disease processes in vitro. Finally, we propose a strategy for creating an organ-specific diabetic vasculopathy model, serving as a valuable template for modelling other types of vascular complications in cardiovascular diseases by incorporating disease-specific stressors and organotypic modifications. Graphical abstract |
| first_indexed | 2024-03-10T22:12:41Z |
| format | Article |
| id | doaj.art-b25058a85f684c40b5f44aded041a860 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-03-10T22:12:41Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-b25058a85f684c40b5f44aded041a8602023-11-19T12:33:32ZengBMCStem Cell Research & Therapy1757-65122023-10-0114111410.1186/s13287-023-03521-2Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategiesHojjat Naderi-Meshkin0Victoria A. Cornelius1Magdalini Eleftheriadou2Koray Niels Potel3Wiwit Ananda Wahyu Setyaningsih4Andriana Margariti5The Wellcome-Wolfson Institute for Experimental Medicine, Queen’s University BelfastThe Wellcome-Wolfson Institute for Experimental Medicine, Queen’s University BelfastThe Wellcome-Wolfson Institute for Experimental Medicine, Queen’s University BelfastThe Wellcome-Wolfson Institute for Experimental Medicine, Queen’s University BelfastThe Wellcome-Wolfson Institute for Experimental Medicine, Queen’s University BelfastThe Wellcome-Wolfson Institute for Experimental Medicine, Queen’s University BelfastAbstract Understanding mechanisms and manifestations of cardiovascular risk factors, including diabetes, on vascular cells such as endothelial cells, pericytes, and vascular smooth muscle cells, remains elusive partly due to the lack of appropriate disease models. Therefore, here we explore different aspects for the development of advanced 3D in vitro disease models that recapitulate human blood vessel complications using patient-derived induced pluripotent stem cells, which retain the epigenetic, transcriptomic, and metabolic memory of their patient-of-origin. In this review, we highlight the superiority of 3D blood vessel organoids over conventional 2D cell culture systems for vascular research. We outline the key benefits of vascular organoids in both health and disease contexts and discuss the current challenges associated with organoid technology, providing potential solutions. Furthermore, we discuss the diverse applications of vascular organoids and emphasize the importance of incorporating all relevant cellular components in a 3D model to accurately recapitulate vascular pathophysiology. As a specific example, we present a comprehensive overview of diabetic vasculopathy, demonstrating how the interplay of different vascular cell types is critical for the successful modelling of complex disease processes in vitro. Finally, we propose a strategy for creating an organ-specific diabetic vasculopathy model, serving as a valuable template for modelling other types of vascular complications in cardiovascular diseases by incorporating disease-specific stressors and organotypic modifications. Graphical abstracthttps://doi.org/10.1186/s13287-023-03521-2Vascular disease modellingVasculopathyAngiopathyOrgan-specific endothelial dysfunctionPluripotent stem cellsMicrovascular and macrovascular complications |
| spellingShingle | Hojjat Naderi-Meshkin Victoria A. Cornelius Magdalini Eleftheriadou Koray Niels Potel Wiwit Ananda Wahyu Setyaningsih Andriana Margariti Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies Stem Cell Research & Therapy Vascular disease modelling Vasculopathy Angiopathy Organ-specific endothelial dysfunction Pluripotent stem cells Microvascular and macrovascular complications |
| title | Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies |
| title_full | Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies |
| title_fullStr | Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies |
| title_full_unstemmed | Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies |
| title_short | Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies |
| title_sort | vascular organoids unveiling advantages applications challenges and disease modelling strategies |
| topic | Vascular disease modelling Vasculopathy Angiopathy Organ-specific endothelial dysfunction Pluripotent stem cells Microvascular and macrovascular complications |
| url | https://doi.org/10.1186/s13287-023-03521-2 |
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