Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteria
Abstract Background The use of beneficial microorganisms as an alternative for pest control has gained increasing attention. The objective of this study was to screen beneficial rhizosphere bacteria with the ability to enhance tomato anti-herbivore resistance. Results Rhizosphere bacteria in tomato...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03644-3 |
| _version_ | 1817989596654338048 |
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| author | Sumei Ling Yi Zhao Shaozhi Sun Dong Zheng Xiaomin Sun Rensen Zeng Dongmei Chen Yuanyuan Song |
| author_facet | Sumei Ling Yi Zhao Shaozhi Sun Dong Zheng Xiaomin Sun Rensen Zeng Dongmei Chen Yuanyuan Song |
| author_sort | Sumei Ling |
| collection | DOAJ |
| description | Abstract Background The use of beneficial microorganisms as an alternative for pest control has gained increasing attention. The objective of this study was to screen beneficial rhizosphere bacteria with the ability to enhance tomato anti-herbivore resistance. Results Rhizosphere bacteria in tomato field from Fuqing, one of the four locations where rhizosphere bacteria were collected in Fujian, China, enhanced tomato resistance against the tobacco cutworm Spodoptera litura, an important polyphagous pest. Inoculation with the isolate T6–4 obtained from the rhizosphere of tomato field in Fuqing reduced leaf damage and weight gain of S. litura larvae fed on the leaves of inoculated tomato plants by 27% in relative to control. Analysis of 16S rRNA gene sequence identities indicated that the isolate T6–4 was closely related to Stenotrophomonas rhizophila supported with 99.37% sequence similarity. In the presence of S. litura infestation, inoculation with the bacterium led to increases by a 66.9% increase in protease inhibitor activity, 53% in peroxidase activity and 80% in polyphenol oxidase activity in the leaves of inoculated plants as compared to the un-inoculated control. Moreover, the expression levels of defense-related genes encoding allene oxide cyclase (AOC), allene oxide synthase (AOS), lipoxygenase D (LOXD) and proteinase inhibitor (PI-II) in tomato leaves were induced 2.2-, 1.7-, 1.4- and 2.7-fold, respectively by T6–4 inoculation. Conclusion These results showed that the tomato rhizosphere soils harbor beneficial bacteria that can systemically induce jasmonate-dependent anti-herbivore resistance in tomato plants. |
| first_indexed | 2024-04-14T00:49:08Z |
| format | Article |
| id | doaj.art-b2506a5ef595467aa22e71e1ef21d923 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-14T00:49:08Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-b2506a5ef595467aa22e71e1ef21d9232022-12-22T02:21:53ZengBMCBMC Plant Biology1471-22292022-05-0122111010.1186/s12870-022-03644-3Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteriaSumei Ling0Yi Zhao1Shaozhi Sun2Dong Zheng3Xiaomin Sun4Rensen Zeng5Dongmei Chen6Yuanyuan Song7Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Life Sciences, Fujian Agriculture and Forestry UniversityInstitute of Crop Resistance and Chemical Ecology, College of Agriculture, Fujian Agriculture and Forestry UniversityKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Life Sciences, Fujian Agriculture and Forestry UniversityInstitute of Crop Resistance and Chemical Ecology, College of Agriculture, Fujian Agriculture and Forestry UniversityInstitute of Crop Resistance and Chemical Ecology, College of Agriculture, Fujian Agriculture and Forestry UniversityInstitute of Crop Resistance and Chemical Ecology, College of Agriculture, Fujian Agriculture and Forestry UniversityInstitute of Crop Resistance and Chemical Ecology, College of Agriculture, Fujian Agriculture and Forestry UniversityInstitute of Crop Resistance and Chemical Ecology, College of Agriculture, Fujian Agriculture and Forestry UniversityAbstract Background The use of beneficial microorganisms as an alternative for pest control has gained increasing attention. The objective of this study was to screen beneficial rhizosphere bacteria with the ability to enhance tomato anti-herbivore resistance. Results Rhizosphere bacteria in tomato field from Fuqing, one of the four locations where rhizosphere bacteria were collected in Fujian, China, enhanced tomato resistance against the tobacco cutworm Spodoptera litura, an important polyphagous pest. Inoculation with the isolate T6–4 obtained from the rhizosphere of tomato field in Fuqing reduced leaf damage and weight gain of S. litura larvae fed on the leaves of inoculated tomato plants by 27% in relative to control. Analysis of 16S rRNA gene sequence identities indicated that the isolate T6–4 was closely related to Stenotrophomonas rhizophila supported with 99.37% sequence similarity. In the presence of S. litura infestation, inoculation with the bacterium led to increases by a 66.9% increase in protease inhibitor activity, 53% in peroxidase activity and 80% in polyphenol oxidase activity in the leaves of inoculated plants as compared to the un-inoculated control. Moreover, the expression levels of defense-related genes encoding allene oxide cyclase (AOC), allene oxide synthase (AOS), lipoxygenase D (LOXD) and proteinase inhibitor (PI-II) in tomato leaves were induced 2.2-, 1.7-, 1.4- and 2.7-fold, respectively by T6–4 inoculation. Conclusion These results showed that the tomato rhizosphere soils harbor beneficial bacteria that can systemically induce jasmonate-dependent anti-herbivore resistance in tomato plants.https://doi.org/10.1186/s12870-022-03644-3Stenotrophomonas rhizophilaRhizosphere bacteriumTomatoSpodoptera lituraAnti-herbivore defense |
| spellingShingle | Sumei Ling Yi Zhao Shaozhi Sun Dong Zheng Xiaomin Sun Rensen Zeng Dongmei Chen Yuanyuan Song Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteria BMC Plant Biology Stenotrophomonas rhizophila Rhizosphere bacterium Tomato Spodoptera litura Anti-herbivore defense |
| title | Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteria |
| title_full | Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteria |
| title_fullStr | Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteria |
| title_full_unstemmed | Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteria |
| title_short | Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteria |
| title_sort | enhanced anti herbivore defense of tomato plants against spodoptera litura by their rhizosphere bacteria |
| topic | Stenotrophomonas rhizophila Rhizosphere bacterium Tomato Spodoptera litura Anti-herbivore defense |
| url | https://doi.org/10.1186/s12870-022-03644-3 |
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