Transcriptome Analysis of <i>Arabidopsis thaliana</i> Plants Treated with a New Compound Natolen128, Enhancing Salt Stress Tolerance
Salinity stress is a major threat to agriculture and global food security. Chemical priming is a promising approach to improving salinity stress tolerance in plants. To identify small molecules with the capacity to enhance salinity stress tolerance in plants, chemical screening was performed using &...
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MDPI AG
2021-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/5/978 |
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| author | Kaori Sako Chien Van Ha Akihiro Matsui Maho Tanaka Ayato Sato Motoaki Seki |
| author_facet | Kaori Sako Chien Van Ha Akihiro Matsui Maho Tanaka Ayato Sato Motoaki Seki |
| author_sort | Kaori Sako |
| collection | DOAJ |
| description | Salinity stress is a major threat to agriculture and global food security. Chemical priming is a promising approach to improving salinity stress tolerance in plants. To identify small molecules with the capacity to enhance salinity stress tolerance in plants, chemical screening was performed using <i>Arabidopsis thaliana</i>. We screened 6400 compounds from the Nagoya University Institute of Transformative Bio-Molecule (ITbM) chemical library and identified one compound, Natolen128, that enhanced salinity-stress tolerance. Furthermore, we isolated a negative compound of Natolen128, namely Necolen124, that did not enhance salinity stress tolerance, though it has a similar chemical structure to Natolen128. We conducted a transcriptomic analysis of Natolen128 and Necolen124 to investigate how Natolen128 enhances high-salinity stress tolerance. Our data indicated that the expression levels of 330 genes were upregulated by Natolen128 treatment compared with that of Necolen124. Treatment with Natolen128 increased expression of hypoxia-responsive genes including ethylene biosynthetic enzymes and <i>PHYTOGLOBIN</i>, which modulate accumulation of nitric oxide (NO) level. NO was slightly increased in plants treated with Natolen128. These results suggest that Natolen128 may regulate NO accumulation and thus, improve salinity stress tolerance in <i>A. thaliana</i>. |
| first_indexed | 2024-03-10T11:26:08Z |
| format | Article |
| id | doaj.art-b25535ff0e1345da829ac849a455df30 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T11:26:08Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-b25535ff0e1345da829ac849a455df302023-11-21T19:40:09ZengMDPI AGPlants2223-77472021-05-0110597810.3390/plants10050978Transcriptome Analysis of <i>Arabidopsis thaliana</i> Plants Treated with a New Compound Natolen128, Enhancing Salt Stress ToleranceKaori Sako0Chien Van Ha1Akihiro Matsui2Maho Tanaka3Ayato Sato4Motoaki Seki5Department of Advanced Bioscience, Faculty of Agriculture, Kindai University, Nara 631-8505, JapanPlant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama 230-0045, JapanPlant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama 230-0045, JapanPlant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama 230-0045, JapanInstitute of Transformative Bio-Molecules (ITbM), Nagoya University, Nagoya 464-8601, JapanPlant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama 230-0045, JapanSalinity stress is a major threat to agriculture and global food security. Chemical priming is a promising approach to improving salinity stress tolerance in plants. To identify small molecules with the capacity to enhance salinity stress tolerance in plants, chemical screening was performed using <i>Arabidopsis thaliana</i>. We screened 6400 compounds from the Nagoya University Institute of Transformative Bio-Molecule (ITbM) chemical library and identified one compound, Natolen128, that enhanced salinity-stress tolerance. Furthermore, we isolated a negative compound of Natolen128, namely Necolen124, that did not enhance salinity stress tolerance, though it has a similar chemical structure to Natolen128. We conducted a transcriptomic analysis of Natolen128 and Necolen124 to investigate how Natolen128 enhances high-salinity stress tolerance. Our data indicated that the expression levels of 330 genes were upregulated by Natolen128 treatment compared with that of Necolen124. Treatment with Natolen128 increased expression of hypoxia-responsive genes including ethylene biosynthetic enzymes and <i>PHYTOGLOBIN</i>, which modulate accumulation of nitric oxide (NO) level. NO was slightly increased in plants treated with Natolen128. These results suggest that Natolen128 may regulate NO accumulation and thus, improve salinity stress tolerance in <i>A. thaliana</i>.https://www.mdpi.com/2223-7747/10/5/978salinity stresschemical screeningnitric oxide |
| spellingShingle | Kaori Sako Chien Van Ha Akihiro Matsui Maho Tanaka Ayato Sato Motoaki Seki Transcriptome Analysis of <i>Arabidopsis thaliana</i> Plants Treated with a New Compound Natolen128, Enhancing Salt Stress Tolerance Plants salinity stress chemical screening nitric oxide |
| title | Transcriptome Analysis of <i>Arabidopsis thaliana</i> Plants Treated with a New Compound Natolen128, Enhancing Salt Stress Tolerance |
| title_full | Transcriptome Analysis of <i>Arabidopsis thaliana</i> Plants Treated with a New Compound Natolen128, Enhancing Salt Stress Tolerance |
| title_fullStr | Transcriptome Analysis of <i>Arabidopsis thaliana</i> Plants Treated with a New Compound Natolen128, Enhancing Salt Stress Tolerance |
| title_full_unstemmed | Transcriptome Analysis of <i>Arabidopsis thaliana</i> Plants Treated with a New Compound Natolen128, Enhancing Salt Stress Tolerance |
| title_short | Transcriptome Analysis of <i>Arabidopsis thaliana</i> Plants Treated with a New Compound Natolen128, Enhancing Salt Stress Tolerance |
| title_sort | transcriptome analysis of i arabidopsis thaliana i plants treated with a new compound natolen128 enhancing salt stress tolerance |
| topic | salinity stress chemical screening nitric oxide |
| url | https://www.mdpi.com/2223-7747/10/5/978 |
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