Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats
Skeletal muscle atrophy is a condition characterized by a loss of muscle mass and muscle strength caused by an imbalance between protein synthesis and protein degradation. Muscle atrophy is often associated with a loss of bone mass manifesting as osteoporosis. The aim of this study was to evaluate i...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/4/3765 |
| _version_ | 1797620490424025088 |
|---|---|
| author | Francesca Bosco Lorenza Guarnieri Saverio Nucera Miriam Scicchitano Stefano Ruga Antonio Cardamone Samantha Maurotti Cristina Russo Anna Rita Coppoletta Roberta Macrì Irene Bava Federica Scarano Fabio Castagna Maria Serra Rosamaria Caminiti Jessica Maiuolo Francesca Oppedisano Sara Ilari Filomena Lauro Luigi Giancotti Carolina Muscoli Cristina Carresi Ernesto Palma Micaela Gliozzi Vincenzo Musolino Vincenzo Mollace |
| author_facet | Francesca Bosco Lorenza Guarnieri Saverio Nucera Miriam Scicchitano Stefano Ruga Antonio Cardamone Samantha Maurotti Cristina Russo Anna Rita Coppoletta Roberta Macrì Irene Bava Federica Scarano Fabio Castagna Maria Serra Rosamaria Caminiti Jessica Maiuolo Francesca Oppedisano Sara Ilari Filomena Lauro Luigi Giancotti Carolina Muscoli Cristina Carresi Ernesto Palma Micaela Gliozzi Vincenzo Musolino Vincenzo Mollace |
| author_sort | Francesca Bosco |
| collection | DOAJ |
| description | Skeletal muscle atrophy is a condition characterized by a loss of muscle mass and muscle strength caused by an imbalance between protein synthesis and protein degradation. Muscle atrophy is often associated with a loss of bone mass manifesting as osteoporosis. The aim of this study was to evaluate if chronic constriction injury (CCI) of the sciatic nerve in rats can be a valid model to study muscle atrophy and consequent osteoporosis. Body weight and body composition were assessed weekly. Magnetic resonance imaging (MRI) was performed on day zero before ligation and day 28 before sacrifice. Catabolic markers were assessed via Western blot and Quantitative Real-time PCR. After the sacrifice, a morphological analysis of the gastrocnemius muscle and Micro-Computed Tomography (Micro-CT) on the tibia bone were performed. Rats that underwent CCI had a lower body weight increase on day 28 compared to the naive group of rats (<i>p</i> < 0.001). Increases in lean body mass and fat mass were also significantly lower in the CCI group (<i>p</i> < 0.001). The weight of skeletal muscles was found to be significantly lower in the ipsilateral hindlimb compared to that of contralateral muscles; furthermore, the cross-sectional area of muscle fibers decreased significantly in the ipsilateral gastrocnemius. The CCI of the sciatic nerve induced a statistically significant increase in autophagic and UPS (Ubiquitin Proteasome System) markers and a statistically significant increase in Pax-7 (Paired Box-7) expression. Micro-CT showed a statistically significant decrease in the bone parameters of the ipsilateral tibial bone. Chronic nerve constriction appeared to be a valid model for inducing the condition of muscle atrophy, also causing changes in bone microstructure and leading to osteoporosis. Therefore, sciatic nerve constriction could be a valid approach to study muscle–bone crosstalk and to identify new strategies to prevent osteosarcopenia. |
| first_indexed | 2024-03-11T08:42:12Z |
| format | Article |
| id | doaj.art-b25c43428d9b4ac3bc3311be8de5ab08 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:42:12Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b25c43428d9b4ac3bc3311be8de5ab082023-11-16T21:04:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244376510.3390/ijms24043765Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in RatsFrancesca Bosco0Lorenza Guarnieri1Saverio Nucera2Miriam Scicchitano3Stefano Ruga4Antonio Cardamone5Samantha Maurotti6Cristina Russo7Anna Rita Coppoletta8Roberta Macrì9Irene Bava10Federica Scarano11Fabio Castagna12Maria Serra13Rosamaria Caminiti14Jessica Maiuolo15Francesca Oppedisano16Sara Ilari17Filomena Lauro18Luigi Giancotti19Carolina Muscoli20Cristina Carresi21Ernesto Palma22Micaela Gliozzi23Vincenzo Musolino24Vincenzo Mollace25Department of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Science, University Magna Grecia, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Science, University Magna Grecia, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyLaboratory of Pharmaceutical Biology, Department of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH) Center, University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyHenry and Amelia Nasrallah Center for Neuroscience, Department of Pharmacology and Physiology, Saint Louis University School of Medicine, Grand Blvd, St. Louis, MO 63104, USAHenry and Amelia Nasrallah Center for Neuroscience, Department of Pharmacology and Physiology, Saint Louis University School of Medicine, Grand Blvd, St. Louis, MO 63104, USADepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyLaboratory of Pharmaceutical Biology, Department of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH) Center, University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Sciences, Institute of Research for Food Safety and Health (IRC-FSH), University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalySkeletal muscle atrophy is a condition characterized by a loss of muscle mass and muscle strength caused by an imbalance between protein synthesis and protein degradation. Muscle atrophy is often associated with a loss of bone mass manifesting as osteoporosis. The aim of this study was to evaluate if chronic constriction injury (CCI) of the sciatic nerve in rats can be a valid model to study muscle atrophy and consequent osteoporosis. Body weight and body composition were assessed weekly. Magnetic resonance imaging (MRI) was performed on day zero before ligation and day 28 before sacrifice. Catabolic markers were assessed via Western blot and Quantitative Real-time PCR. After the sacrifice, a morphological analysis of the gastrocnemius muscle and Micro-Computed Tomography (Micro-CT) on the tibia bone were performed. Rats that underwent CCI had a lower body weight increase on day 28 compared to the naive group of rats (<i>p</i> < 0.001). Increases in lean body mass and fat mass were also significantly lower in the CCI group (<i>p</i> < 0.001). The weight of skeletal muscles was found to be significantly lower in the ipsilateral hindlimb compared to that of contralateral muscles; furthermore, the cross-sectional area of muscle fibers decreased significantly in the ipsilateral gastrocnemius. The CCI of the sciatic nerve induced a statistically significant increase in autophagic and UPS (Ubiquitin Proteasome System) markers and a statistically significant increase in Pax-7 (Paired Box-7) expression. Micro-CT showed a statistically significant decrease in the bone parameters of the ipsilateral tibial bone. Chronic nerve constriction appeared to be a valid model for inducing the condition of muscle atrophy, also causing changes in bone microstructure and leading to osteoporosis. Therefore, sciatic nerve constriction could be a valid approach to study muscle–bone crosstalk and to identify new strategies to prevent osteosarcopenia.https://www.mdpi.com/1422-0067/24/4/3765chronic constriction injurymuscle atrophymuscle atrophy modelosteoporosisosteopeniaosteosarcopenia |
| spellingShingle | Francesca Bosco Lorenza Guarnieri Saverio Nucera Miriam Scicchitano Stefano Ruga Antonio Cardamone Samantha Maurotti Cristina Russo Anna Rita Coppoletta Roberta Macrì Irene Bava Federica Scarano Fabio Castagna Maria Serra Rosamaria Caminiti Jessica Maiuolo Francesca Oppedisano Sara Ilari Filomena Lauro Luigi Giancotti Carolina Muscoli Cristina Carresi Ernesto Palma Micaela Gliozzi Vincenzo Musolino Vincenzo Mollace Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats International Journal of Molecular Sciences chronic constriction injury muscle atrophy muscle atrophy model osteoporosis osteopenia osteosarcopenia |
| title | Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats |
| title_full | Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats |
| title_fullStr | Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats |
| title_full_unstemmed | Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats |
| title_short | Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats |
| title_sort | pathophysiological aspects of muscle atrophy and osteopenia induced by chronic constriction injury cci of the sciatic nerve in rats |
| topic | chronic constriction injury muscle atrophy muscle atrophy model osteoporosis osteopenia osteosarcopenia |
| url | https://www.mdpi.com/1422-0067/24/4/3765 |
| work_keys_str_mv | AT francescabosco pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT lorenzaguarnieri pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT saverionucera pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT miriamscicchitano pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT stefanoruga pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT antoniocardamone pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT samanthamaurotti pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT cristinarusso pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT annaritacoppoletta pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT robertamacri pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT irenebava pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT federicascarano pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT fabiocastagna pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT mariaserra pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT rosamariacaminiti pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT jessicamaiuolo pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT francescaoppedisano pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT sarailari pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT filomenalauro pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT luigigiancotti pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT carolinamuscoli pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT cristinacarresi pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT ernestopalma pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT micaelagliozzi pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT vincenzomusolino pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats AT vincenzomollace pathophysiologicalaspectsofmuscleatrophyandosteopeniainducedbychronicconstrictioninjurycciofthesciaticnerveinrats |