The Effect of Plasma-Treated Water on Microbial Growth and Biosynthesis of Gamma-Decalactones by <i>Yarrowia lipolytica</i> Yeast

In recent years, the production of plasma-treated water (PTW) by low-temperature low-pressure glow plasma (LPGP) has been increasingly gaining in popularity. LPGP-treated water changes its physical and physiochemical properties compared to standard distilled water. In this study, a non-conventional...

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Main Authors: Jolanta Małajowicz, Karen Khachatryan, Zdzisław Oszczęda, Piotr Karpiński, Agata Fabiszewska, Bartłomiej Zieniuk, Konrad Krysowaty
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/24/20/15204
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author Jolanta Małajowicz
Karen Khachatryan
Zdzisław Oszczęda
Piotr Karpiński
Agata Fabiszewska
Bartłomiej Zieniuk
Konrad Krysowaty
author_facet Jolanta Małajowicz
Karen Khachatryan
Zdzisław Oszczęda
Piotr Karpiński
Agata Fabiszewska
Bartłomiej Zieniuk
Konrad Krysowaty
author_sort Jolanta Małajowicz
collection DOAJ
description In recent years, the production of plasma-treated water (PTW) by low-temperature low-pressure glow plasma (LPGP) has been increasingly gaining in popularity. LPGP-treated water changes its physical and physiochemical properties compared to standard distilled water. In this study, a non-conventional lipolytic yeast species <i>Yarrowia lipolytica</i> was cultivated in culture media based on Nantes plasma water with heightened singlet oxygen content (Nantes PW) or in water treated with low-temperature, low-pressure glow plasma while in contact with air (PWTA) or nitrogen (PWTN). The research aimed to assess the influence of culture conditions on castor oil biotransformation to gamma-decalactone (GDL) and other secondary metabolites in media based on nanowater. The Nantes plasma water-based medium attained the highest concentration of gamma-decalactone (4.81 ± 0.51 g/L at 144 h of culture), maximum biomass concentration and biomass yield from the substrate. The amplified activity of lipases in the nanowater-based medium, in comparison to the control medium, is encouraging from the perspective of GDL biosynthesis, relying on the biotransformation of ricinoleic acid, which is the primary component of castor oil. Although lipid hydrolysis was enhanced, this step seemed not crucial for GDL concentration. Interestingly, the study validates the significance of oxygen in β-oxidation enzymes and its role in the bioconversion of ricinoleic acid to GDL and other lactones. Specifically, media with higher oxygen content (WPTA) and Nantes plasma water resulted in remarkably high concentrations of four lactones: gamma-decalactone, 3-hydroxy-gamma-decalactone, dec-2-en-4-olide and dec-3-en-4-olide.
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spelling doaj.art-b25c5bf9488947de8040b0083cd7124b2023-11-19T16:43:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201520410.3390/ijms242015204The Effect of Plasma-Treated Water on Microbial Growth and Biosynthesis of Gamma-Decalactones by <i>Yarrowia lipolytica</i> YeastJolanta Małajowicz0Karen Khachatryan1Zdzisław Oszczęda2Piotr Karpiński3Agata Fabiszewska4Bartłomiej Zieniuk5Konrad Krysowaty6Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska Street 159C, 02-776 Warsaw, PolandLaboratory of Nanomaterials and Nanotechnology, Faculty of Food Technology, University of Agriculture in Cracow, Balicka Street 122, 30-149 Cracow, PolandNantes Nanotechnological Systems, Dolne Młyny Street 21, 59-700 Bolesławiec, PolandFaculty of Computer Science and Technology, Lomza State University of Applied Sciences, Akademicka Street 1, 18-400 Łomża, PolandDepartment of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska Street 159C, 02-776 Warsaw, PolandDepartment of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska Street 159C, 02-776 Warsaw, PolandFaculty of Biology and Biotechnology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warsaw, PolandIn recent years, the production of plasma-treated water (PTW) by low-temperature low-pressure glow plasma (LPGP) has been increasingly gaining in popularity. LPGP-treated water changes its physical and physiochemical properties compared to standard distilled water. In this study, a non-conventional lipolytic yeast species <i>Yarrowia lipolytica</i> was cultivated in culture media based on Nantes plasma water with heightened singlet oxygen content (Nantes PW) or in water treated with low-temperature, low-pressure glow plasma while in contact with air (PWTA) or nitrogen (PWTN). The research aimed to assess the influence of culture conditions on castor oil biotransformation to gamma-decalactone (GDL) and other secondary metabolites in media based on nanowater. The Nantes plasma water-based medium attained the highest concentration of gamma-decalactone (4.81 ± 0.51 g/L at 144 h of culture), maximum biomass concentration and biomass yield from the substrate. The amplified activity of lipases in the nanowater-based medium, in comparison to the control medium, is encouraging from the perspective of GDL biosynthesis, relying on the biotransformation of ricinoleic acid, which is the primary component of castor oil. Although lipid hydrolysis was enhanced, this step seemed not crucial for GDL concentration. Interestingly, the study validates the significance of oxygen in β-oxidation enzymes and its role in the bioconversion of ricinoleic acid to GDL and other lactones. Specifically, media with higher oxygen content (WPTA) and Nantes plasma water resulted in remarkably high concentrations of four lactones: gamma-decalactone, 3-hydroxy-gamma-decalactone, dec-2-en-4-olide and dec-3-en-4-olide.https://www.mdpi.com/1422-0067/24/20/15204glow plasmaplasma-treated watergamma-decalactoneslipasesβ-oxidation<i>Yarrowia lipolytica</i>
spellingShingle Jolanta Małajowicz
Karen Khachatryan
Zdzisław Oszczęda
Piotr Karpiński
Agata Fabiszewska
Bartłomiej Zieniuk
Konrad Krysowaty
The Effect of Plasma-Treated Water on Microbial Growth and Biosynthesis of Gamma-Decalactones by <i>Yarrowia lipolytica</i> Yeast
International Journal of Molecular Sciences
glow plasma
plasma-treated water
gamma-decalactones
lipases
β-oxidation
<i>Yarrowia lipolytica</i>
title The Effect of Plasma-Treated Water on Microbial Growth and Biosynthesis of Gamma-Decalactones by <i>Yarrowia lipolytica</i> Yeast
title_full The Effect of Plasma-Treated Water on Microbial Growth and Biosynthesis of Gamma-Decalactones by <i>Yarrowia lipolytica</i> Yeast
title_fullStr The Effect of Plasma-Treated Water on Microbial Growth and Biosynthesis of Gamma-Decalactones by <i>Yarrowia lipolytica</i> Yeast
title_full_unstemmed The Effect of Plasma-Treated Water on Microbial Growth and Biosynthesis of Gamma-Decalactones by <i>Yarrowia lipolytica</i> Yeast
title_short The Effect of Plasma-Treated Water on Microbial Growth and Biosynthesis of Gamma-Decalactones by <i>Yarrowia lipolytica</i> Yeast
title_sort effect of plasma treated water on microbial growth and biosynthesis of gamma decalactones by i yarrowia lipolytica i yeast
topic glow plasma
plasma-treated water
gamma-decalactones
lipases
β-oxidation
<i>Yarrowia lipolytica</i>
url https://www.mdpi.com/1422-0067/24/20/15204
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