Building Pluripotency Identity in the Early Embryo and Derived Stem Cells
The fusion of two highly differentiated cells, an oocyte with a spermatozoon, gives rise to the zygote, a single totipotent cell, which has the capability to develop into a complete, fully functional organism. Then, as development proceeds, a series of programmed cell divisions occur whereby the ari...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/8/2049 |
| _version_ | 1797524327865778176 |
|---|---|
| author | Paola Rebuzzini Maurizio Zuccotti Silvia Garagna |
| author_facet | Paola Rebuzzini Maurizio Zuccotti Silvia Garagna |
| author_sort | Paola Rebuzzini |
| collection | DOAJ |
| description | The fusion of two highly differentiated cells, an oocyte with a spermatozoon, gives rise to the zygote, a single totipotent cell, which has the capability to develop into a complete, fully functional organism. Then, as development proceeds, a series of programmed cell divisions occur whereby the arising cells progressively acquire their own cellular and molecular identity, and totipotency narrows until when pluripotency is achieved. The path towards pluripotency involves transcriptome modulation, remodeling of the chromatin epigenetic landscape to which external modulators contribute. Both human and mouse embryos are a source of different types of pluripotent stem cells whose characteristics can be captured and maintained in vitro. The main aim of this review is to address the cellular properties and the molecular signature of the emerging cells during mouse and human early development, highlighting similarities and differences between the two species and between the embryos and their cognate stem cells. |
| first_indexed | 2024-03-10T08:55:47Z |
| format | Article |
| id | doaj.art-b25cc1c182494430a664bd8ed2ed59a5 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T08:55:47Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b25cc1c182494430a664bd8ed2ed59a52023-11-22T07:11:00ZengMDPI AGCells2073-44092021-08-01108204910.3390/cells10082049Building Pluripotency Identity in the Early Embryo and Derived Stem CellsPaola Rebuzzini0Maurizio Zuccotti1Silvia Garagna2Laboratory of Developmental Biology, Department of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia, Via Ferrata 9, 27100 Pavia, ItalyLaboratory of Developmental Biology, Department of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia, Via Ferrata 9, 27100 Pavia, ItalyLaboratory of Developmental Biology, Department of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia, Via Ferrata 9, 27100 Pavia, ItalyThe fusion of two highly differentiated cells, an oocyte with a spermatozoon, gives rise to the zygote, a single totipotent cell, which has the capability to develop into a complete, fully functional organism. Then, as development proceeds, a series of programmed cell divisions occur whereby the arising cells progressively acquire their own cellular and molecular identity, and totipotency narrows until when pluripotency is achieved. The path towards pluripotency involves transcriptome modulation, remodeling of the chromatin epigenetic landscape to which external modulators contribute. Both human and mouse embryos are a source of different types of pluripotent stem cells whose characteristics can be captured and maintained in vitro. The main aim of this review is to address the cellular properties and the molecular signature of the emerging cells during mouse and human early development, highlighting similarities and differences between the two species and between the embryos and their cognate stem cells.https://www.mdpi.com/2073-4409/10/8/2049peri-implantation embryoEGAlineage specificationpluripotent stem cellspluripotency transcriptional networksepigenetic |
| spellingShingle | Paola Rebuzzini Maurizio Zuccotti Silvia Garagna Building Pluripotency Identity in the Early Embryo and Derived Stem Cells Cells peri-implantation embryo EGA lineage specification pluripotent stem cells pluripotency transcriptional networks epigenetic |
| title | Building Pluripotency Identity in the Early Embryo and Derived Stem Cells |
| title_full | Building Pluripotency Identity in the Early Embryo and Derived Stem Cells |
| title_fullStr | Building Pluripotency Identity in the Early Embryo and Derived Stem Cells |
| title_full_unstemmed | Building Pluripotency Identity in the Early Embryo and Derived Stem Cells |
| title_short | Building Pluripotency Identity in the Early Embryo and Derived Stem Cells |
| title_sort | building pluripotency identity in the early embryo and derived stem cells |
| topic | peri-implantation embryo EGA lineage specification pluripotent stem cells pluripotency transcriptional networks epigenetic |
| url | https://www.mdpi.com/2073-4409/10/8/2049 |
| work_keys_str_mv | AT paolarebuzzini buildingpluripotencyidentityintheearlyembryoandderivedstemcells AT mauriziozuccotti buildingpluripotencyidentityintheearlyembryoandderivedstemcells AT silviagaragna buildingpluripotencyidentityintheearlyembryoandderivedstemcells |