Bio-Synthesis of copper nanoparticles (CuNPs) using Garlic extract to investigate antibacterial activity
Bio-synthesis of metal nanoparticles (CuNPs) is regarded as one of the recently developed, economic and environmentally benign method. In the present investigation, Copper nanoparticles were synthesized reacting garlic (Allium sativum) extract with Copper Sulphate (CuSO4∙5H2O) solution over magneti...
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| Format: | Article |
| Language: | English |
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Department of Physics, Mahendra Morang Adarsh Multiple Campus, Tribhuvan University
2020-01-01
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| Series: | Bibechana |
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| Online Access: | https://www.nepjol.info/index.php/BIBECHANA/article/view/23485 |
| _version_ | 1797194069421588480 |
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| author | Sharmila Pradhan Amatya Leela Pradhan Joshi |
| author_facet | Sharmila Pradhan Amatya Leela Pradhan Joshi |
| author_sort | Sharmila Pradhan Amatya |
| collection | DOAJ |
| description |
Bio-synthesis of metal nanoparticles (CuNPs) is regarded as one of the recently developed, economic and environmentally benign method. In the present investigation, Copper nanoparticles were synthesized reacting garlic (Allium sativum) extract with Copper Sulphate (CuSO4∙5H2O) solution over magnetic stirrer at 80 °C for 1 hour. So-prepared CuNPs were studied by observing the color change at various time intervals. Further, the nanoparticles were characterized using UV-Visible spectroscopy, Energy Dispersive X-ray spectroscopy (EDX) and Fourier Transform Infrared spectroscopy (FTIR). The results of UV-Vis spectroscopy clearly showed presence of absorption peak at 595 nm which confirmed the formation of copper nanoparticles. Likewise, the EDX spectrum depicts the presence of optical band at 8 eV which is the characteristic peak of Copper consisting of 38.747 % by weight and FTIR spectra revealed presence of various phytochemicals possessing characteristic functional groups such as carbonyl and phenolic at the surface of CuNPs. Thus, natural products available in the garlic extract help in reduction and stabilization of Copper nanoparticles. The antibacterial activity of Copper nanoparticles was investigated against Gram +ve (Staphylococcus aureus) and Gram –ve bacteria (Escherichia coli) using Agarwell diffusion method. The results of antibacterial test showed that CuNPs were found to be much sensitive towards Gram –ve bacteria compared to gram +ve bacteria.
BIBECHANA 17 (2020) 12-18
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| first_indexed | 2024-04-10T05:27:14Z |
| format | Article |
| id | doaj.art-b25d3536ac154c94a03bd5e6f42c5c63 |
| institution | Directory Open Access Journal |
| issn | 2091-0762 2382-5340 |
| language | English |
| last_indexed | 2024-04-24T05:50:25Z |
| publishDate | 2020-01-01 |
| publisher | Department of Physics, Mahendra Morang Adarsh Multiple Campus, Tribhuvan University |
| record_format | Article |
| series | Bibechana |
| spelling | doaj.art-b25d3536ac154c94a03bd5e6f42c5c632024-04-23T13:05:16ZengDepartment of Physics, Mahendra Morang Adarsh Multiple Campus, Tribhuvan UniversityBibechana2091-07622382-53402020-01-011710.3126/bibechana.v17i0.2348520876Bio-Synthesis of copper nanoparticles (CuNPs) using Garlic extract to investigate antibacterial activitySharmila Pradhan Amatya0Leela Pradhan Joshi1Chemistry Department, Amrit Campus, Tribhuvan University, KathmanduPhysics Department, Amrit Campus, Tribhuvan University, Kathmandu Bio-synthesis of metal nanoparticles (CuNPs) is regarded as one of the recently developed, economic and environmentally benign method. In the present investigation, Copper nanoparticles were synthesized reacting garlic (Allium sativum) extract with Copper Sulphate (CuSO4∙5H2O) solution over magnetic stirrer at 80 °C for 1 hour. So-prepared CuNPs were studied by observing the color change at various time intervals. Further, the nanoparticles were characterized using UV-Visible spectroscopy, Energy Dispersive X-ray spectroscopy (EDX) and Fourier Transform Infrared spectroscopy (FTIR). The results of UV-Vis spectroscopy clearly showed presence of absorption peak at 595 nm which confirmed the formation of copper nanoparticles. Likewise, the EDX spectrum depicts the presence of optical band at 8 eV which is the characteristic peak of Copper consisting of 38.747 % by weight and FTIR spectra revealed presence of various phytochemicals possessing characteristic functional groups such as carbonyl and phenolic at the surface of CuNPs. Thus, natural products available in the garlic extract help in reduction and stabilization of Copper nanoparticles. The antibacterial activity of Copper nanoparticles was investigated against Gram +ve (Staphylococcus aureus) and Gram –ve bacteria (Escherichia coli) using Agarwell diffusion method. The results of antibacterial test showed that CuNPs were found to be much sensitive towards Gram –ve bacteria compared to gram +ve bacteria. BIBECHANA 17 (2020) 12-18 https://www.nepjol.info/index.php/BIBECHANA/article/view/23485Allium sativum, Copper nanoparticles, EDX, FTIR |
| spellingShingle | Sharmila Pradhan Amatya Leela Pradhan Joshi Bio-Synthesis of copper nanoparticles (CuNPs) using Garlic extract to investigate antibacterial activity Bibechana Allium sativum, Copper nanoparticles, EDX, FTIR |
| title | Bio-Synthesis of copper nanoparticles (CuNPs) using Garlic extract to investigate antibacterial activity |
| title_full | Bio-Synthesis of copper nanoparticles (CuNPs) using Garlic extract to investigate antibacterial activity |
| title_fullStr | Bio-Synthesis of copper nanoparticles (CuNPs) using Garlic extract to investigate antibacterial activity |
| title_full_unstemmed | Bio-Synthesis of copper nanoparticles (CuNPs) using Garlic extract to investigate antibacterial activity |
| title_short | Bio-Synthesis of copper nanoparticles (CuNPs) using Garlic extract to investigate antibacterial activity |
| title_sort | bio synthesis of copper nanoparticles cunps using garlic extract to investigate antibacterial activity |
| topic | Allium sativum, Copper nanoparticles, EDX, FTIR |
| url | https://www.nepjol.info/index.php/BIBECHANA/article/view/23485 |
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