Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies[S]
Ceramides (Cers) are important in embryogenesis, but no comprehensive analysis of gene expression for Cer metabolism nor the Cer amounts and subspecies has been conducted with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies (EBs). Measuring the mRNA levels by quantitati...
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| Format: | Article |
| Language: | English |
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Elsevier
2010-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752030506X |
| _version_ | 1818726526027300864 |
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| author | Hyejung Park Christopher A. Haynes Alison V. Nairn Michael Kulik Stephen Dalton Kelley Moremen Alfred H. Merrill, Jr. |
| author_facet | Hyejung Park Christopher A. Haynes Alison V. Nairn Michael Kulik Stephen Dalton Kelley Moremen Alfred H. Merrill, Jr. |
| author_sort | Hyejung Park |
| collection | DOAJ |
| description | Ceramides (Cers) are important in embryogenesis, but no comprehensive analysis of gene expression for Cer metabolism nor the Cer amounts and subspecies has been conducted with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies (EBs). Measuring the mRNA levels by quantitative RT-PCR and the amounts of the respective metabolites by LC-ESI/MS/MS, notable differences between R1 mESCs and EBs were: EBs have higher mRNAs for CerS1 and CerS3, which synthesize C18- and C≥24-carbons dihydroceramides (DH)Cer, respectively; EBs have higher CerS2 (for C24:0- and C24:1-); and EBs have lower CerS5 + CerS6 (for C16-). In agreement with these findings, EBs have (DH)Cer with higher proportions of C18-, C24- and C26- and less C16-fatty acids, and longer (DH)Cer are also seen in monohexosylCers and sphingomyelins. EBs had higher mRNAs for fatty acyl-CoA elongases that produce C18-, C24-, and C26-fatty acyl-CoAs (Elovl3 and Elovl6), and higher amounts of these cosubstrates for CerS. Thus, these studies have found generally good agreement between genomic and metabolomic data in defining that conversion of mESCs to EBs is accompanied by a large number of changes in gene expression and subspecies distributions for both sphingolipids and fatty acyl-CoAs. |
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| format | Article |
| id | doaj.art-b25da03f547d4e6e816795f313239520 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T21:59:36Z |
| publishDate | 2010-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-b25da03f547d4e6e816795f3132395202022-12-21T21:31:02ZengElsevierJournal of Lipid Research0022-22752010-03-01513480489Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies[S]Hyejung Park0Christopher A. Haynes1Alison V. Nairn2Michael Kulik3Stephen Dalton4Kelley Moremen5Alfred H. Merrill, Jr.6School of Biology & Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332School of Biology & Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332Department of Biochemistry and Molecular Biology and the Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602Department of Biochemistry and Molecular Biology and the Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602Department of Biochemistry and Molecular Biology and the Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602Department of Biochemistry and Molecular Biology and the Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602To whom correspondence should be addressed; School of Biology & Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332Ceramides (Cers) are important in embryogenesis, but no comprehensive analysis of gene expression for Cer metabolism nor the Cer amounts and subspecies has been conducted with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies (EBs). Measuring the mRNA levels by quantitative RT-PCR and the amounts of the respective metabolites by LC-ESI/MS/MS, notable differences between R1 mESCs and EBs were: EBs have higher mRNAs for CerS1 and CerS3, which synthesize C18- and C≥24-carbons dihydroceramides (DH)Cer, respectively; EBs have higher CerS2 (for C24:0- and C24:1-); and EBs have lower CerS5 + CerS6 (for C16-). In agreement with these findings, EBs have (DH)Cer with higher proportions of C18-, C24- and C26- and less C16-fatty acids, and longer (DH)Cer are also seen in monohexosylCers and sphingomyelins. EBs had higher mRNAs for fatty acyl-CoA elongases that produce C18-, C24-, and C26-fatty acyl-CoAs (Elovl3 and Elovl6), and higher amounts of these cosubstrates for CerS. Thus, these studies have found generally good agreement between genomic and metabolomic data in defining that conversion of mESCs to EBs is accompanied by a large number of changes in gene expression and subspecies distributions for both sphingolipids and fatty acyl-CoAs.http://www.sciencedirect.com/science/article/pii/S002222752030506Xembryonic stem cellembryoid bodysphingolipiddifferentiationceramide synthasefatty acyl-CoA elongase |
| spellingShingle | Hyejung Park Christopher A. Haynes Alison V. Nairn Michael Kulik Stephen Dalton Kelley Moremen Alfred H. Merrill, Jr. Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies[S] Journal of Lipid Research embryonic stem cell embryoid body sphingolipid differentiation ceramide synthase fatty acyl-CoA elongase |
| title | Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies[S] |
| title_full | Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies[S] |
| title_fullStr | Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies[S] |
| title_full_unstemmed | Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies[S] |
| title_short | Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies[S] |
| title_sort | transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies s |
| topic | embryonic stem cell embryoid body sphingolipid differentiation ceramide synthase fatty acyl-CoA elongase |
| url | http://www.sciencedirect.com/science/article/pii/S002222752030506X |
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