Genital <i>Brucella suis</i> Biovar 2 Infection of Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany (Italy)
Brucellosis is a zoonosis caused by different <i>Brucella</i> species. Wild boar (<i>Sus scrofa</i>) could be infected by some species and represents an important reservoir, especially for <i>B. suis</i> biovar 2. This study aimed to investigate the prevalence of...
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MDPI AG
2021-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/3/582 |
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| author | Giovanni Cilia Filippo Fratini Barbara Turchi Marta Angelini Domenico Cerri Fabrizio Bertelloni |
| author_facet | Giovanni Cilia Filippo Fratini Barbara Turchi Marta Angelini Domenico Cerri Fabrizio Bertelloni |
| author_sort | Giovanni Cilia |
| collection | DOAJ |
| description | Brucellosis is a zoonosis caused by different <i>Brucella</i> species. Wild boar (<i>Sus scrofa</i>) could be infected by some species and represents an important reservoir, especially for <i>B. suis</i> biovar 2. This study aimed to investigate the prevalence of <i>Brucella</i> spp. by serological and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. From 287 animals, sera, lymph nodes, livers, spleens, and reproductive system organs were collected. Within sera, 16 (5.74%) were positive to both rose bengal test (RBT) and complement fixation test (CFT), with titres ranging from 1:4 to 1:16 (corresponding to 20 and 80 ICFTU/mL, respectively). <i>Brucella</i> spp. DNA was detected in four lymph nodes (1.40%), five epididymides (1.74%), and one fetus pool (2.22%). All positive PCR samples belonged to <i>Brucella suis</i> biovar 2. The results of this investigation confirmed that wild boar represents a host for <i>B.</i><i>suis</i> biovar. 2 and plays an important role in the epidemiology of brucellosis in central Italy. Additionally, epididymis localization confirms the possible venereal transmission. |
| first_indexed | 2024-03-10T13:18:39Z |
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| id | doaj.art-b25e717a64b6485795864506026d1a6f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:18:39Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b25e717a64b6485795864506026d1a6f2023-11-21T10:13:14ZengMDPI AGMicroorganisms2076-26072021-03-019358210.3390/microorganisms9030582Genital <i>Brucella suis</i> Biovar 2 Infection of Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany (Italy)Giovanni Cilia0Filippo Fratini1Barbara Turchi2Marta Angelini3Domenico Cerri4Fabrizio Bertelloni5Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyBrucellosis is a zoonosis caused by different <i>Brucella</i> species. Wild boar (<i>Sus scrofa</i>) could be infected by some species and represents an important reservoir, especially for <i>B. suis</i> biovar 2. This study aimed to investigate the prevalence of <i>Brucella</i> spp. by serological and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. From 287 animals, sera, lymph nodes, livers, spleens, and reproductive system organs were collected. Within sera, 16 (5.74%) were positive to both rose bengal test (RBT) and complement fixation test (CFT), with titres ranging from 1:4 to 1:16 (corresponding to 20 and 80 ICFTU/mL, respectively). <i>Brucella</i> spp. DNA was detected in four lymph nodes (1.40%), five epididymides (1.74%), and one fetus pool (2.22%). All positive PCR samples belonged to <i>Brucella suis</i> biovar 2. The results of this investigation confirmed that wild boar represents a host for <i>B.</i><i>suis</i> biovar. 2 and plays an important role in the epidemiology of brucellosis in central Italy. Additionally, epididymis localization confirms the possible venereal transmission.https://www.mdpi.com/2076-2607/9/3/582<i>Brucella suis</i> biovar 2wild boarsurveillanceepidemiologyreproductive system |
| spellingShingle | Giovanni Cilia Filippo Fratini Barbara Turchi Marta Angelini Domenico Cerri Fabrizio Bertelloni Genital <i>Brucella suis</i> Biovar 2 Infection of Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany (Italy) Microorganisms <i>Brucella suis</i> biovar 2 wild boar surveillance epidemiology reproductive system |
| title | Genital <i>Brucella suis</i> Biovar 2 Infection of Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany (Italy) |
| title_full | Genital <i>Brucella suis</i> Biovar 2 Infection of Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany (Italy) |
| title_fullStr | Genital <i>Brucella suis</i> Biovar 2 Infection of Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany (Italy) |
| title_full_unstemmed | Genital <i>Brucella suis</i> Biovar 2 Infection of Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany (Italy) |
| title_short | Genital <i>Brucella suis</i> Biovar 2 Infection of Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany (Italy) |
| title_sort | genital i brucella suis i biovar 2 infection of wild boar i sus scrofa i hunted in tuscany italy |
| topic | <i>Brucella suis</i> biovar 2 wild boar surveillance epidemiology reproductive system |
| url | https://www.mdpi.com/2076-2607/9/3/582 |
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