Assessment of a portable lactate meter for field use in the white rhinoceros (<i>Ceratotherium simum</i>)

Blood lactate is a predictor of mortality in critically ill humans and animals. Handheld lactate meters have the potential to be used in the field to evaluate the condition of severely injured rhinoceroses but have not been compared with laboratory-based methods. Agreement between a handheld lactate...

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Main Authors: Georgina C. Cole, Adrian S.W. Tordiffe, Gerhard Steenkamp
Format: Article
Language:English
Published: AOSIS 2017-11-01
Series:Onderstepoort Journal of Veterinary Research
Subjects:
Online Access:https://ojvr.org/index.php/ojvr/article/view/1399
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author Georgina C. Cole
Adrian S.W. Tordiffe
Gerhard Steenkamp
author_facet Georgina C. Cole
Adrian S.W. Tordiffe
Gerhard Steenkamp
author_sort Georgina C. Cole
collection DOAJ
description Blood lactate is a predictor of mortality in critically ill humans and animals. Handheld lactate meters have the potential to be used in the field to evaluate the condition of severely injured rhinoceroses but have not been compared with laboratory-based methods. Agreement between a handheld lactate meter and a laboratory method was assessed, as was the stability of rhino blood lactate in the anticoagulant sodium fluoride/potassium oxalate (fluoride/oxalate). Blood samples were obtained from 53 white rhinos that had been immobilised for management reasons. Lactate was measured by means of a handheld meter using whole blood in heparin (WBHEP), whole blood in fluoride/oxalate (WBFO) and fluoride/oxalate plasma (PFO). Results were recorded in both blood (BL) and plasma (PL) modes and compared to an established laboratory method for measuring plasma lactate. To assess the stability of lactate over time, blood lactate in fluoride/oxalate was measured on the handheld meter at intervals for up to 91 h. Agreement was best using WBFO in PL mode, with small bias (-0.16), tight 95% limits of agreement (LOA) (-1.46, 1.14) and a Pc (95% CI) of 0.97 (0.92, 0.99). The agreement was improved for all sample types when using the PL mode compared to the blood lactate (BL) mode. Blood lactate was stable in fluoride/oxalate for 91 h, with a mean change from baseline of 0.15 (-0.178, 0.478) mmol/L (mean, 95% CI). The handheld meter was found to be suitable for field use in white rhinos but provided more reliable results with the device in PL mode. Furthermore, rhino blood lactate was found to be stable in fluoride/oxalate for as long as 3 days.
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spelling doaj.art-b2600841e96e4ffdabf67f40cc37cff12022-12-22T01:14:51ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352017-11-01841e1e1010.4102/ojvr.v84i1.1399569Assessment of a portable lactate meter for field use in the white rhinoceros (<i>Ceratotherium simum</i>)Georgina C. Cole0Adrian S.W. Tordiffe1Gerhard Steenkamp2Department of Companion Animal Clinical Studies, University of PretoriaDepartment of Paraclinical Sciences, University of PretoriaDepartment of Companion Animal Clinical Studies, University of PretoriaBlood lactate is a predictor of mortality in critically ill humans and animals. Handheld lactate meters have the potential to be used in the field to evaluate the condition of severely injured rhinoceroses but have not been compared with laboratory-based methods. Agreement between a handheld lactate meter and a laboratory method was assessed, as was the stability of rhino blood lactate in the anticoagulant sodium fluoride/potassium oxalate (fluoride/oxalate). Blood samples were obtained from 53 white rhinos that had been immobilised for management reasons. Lactate was measured by means of a handheld meter using whole blood in heparin (WBHEP), whole blood in fluoride/oxalate (WBFO) and fluoride/oxalate plasma (PFO). Results were recorded in both blood (BL) and plasma (PL) modes and compared to an established laboratory method for measuring plasma lactate. To assess the stability of lactate over time, blood lactate in fluoride/oxalate was measured on the handheld meter at intervals for up to 91 h. Agreement was best using WBFO in PL mode, with small bias (-0.16), tight 95% limits of agreement (LOA) (-1.46, 1.14) and a Pc (95% CI) of 0.97 (0.92, 0.99). The agreement was improved for all sample types when using the PL mode compared to the blood lactate (BL) mode. Blood lactate was stable in fluoride/oxalate for 91 h, with a mean change from baseline of 0.15 (-0.178, 0.478) mmol/L (mean, 95% CI). The handheld meter was found to be suitable for field use in white rhinos but provided more reliable results with the device in PL mode. Furthermore, rhino blood lactate was found to be stable in fluoride/oxalate for as long as 3 days.https://ojvr.org/index.php/ojvr/article/view/1399lactateWhite rhinocerospoint of care analyseragreement
spellingShingle Georgina C. Cole
Adrian S.W. Tordiffe
Gerhard Steenkamp
Assessment of a portable lactate meter for field use in the white rhinoceros (<i>Ceratotherium simum</i>)
Onderstepoort Journal of Veterinary Research
lactate
White rhinoceros
point of care analyser
agreement
title Assessment of a portable lactate meter for field use in the white rhinoceros (<i>Ceratotherium simum</i>)
title_full Assessment of a portable lactate meter for field use in the white rhinoceros (<i>Ceratotherium simum</i>)
title_fullStr Assessment of a portable lactate meter for field use in the white rhinoceros (<i>Ceratotherium simum</i>)
title_full_unstemmed Assessment of a portable lactate meter for field use in the white rhinoceros (<i>Ceratotherium simum</i>)
title_short Assessment of a portable lactate meter for field use in the white rhinoceros (<i>Ceratotherium simum</i>)
title_sort assessment of a portable lactate meter for field use in the white rhinoceros i ceratotherium simum i
topic lactate
White rhinoceros
point of care analyser
agreement
url https://ojvr.org/index.php/ojvr/article/view/1399
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AT gerhardsteenkamp assessmentofaportablelactatemeterforfielduseinthewhiterhinocerosiceratotheriumsimumi