3D bioprinting bioglass to construct vascularized full-thickness skin substitutes for wound healing
Constructing three-dimensional (3D) bioprinted skin tissues that accurately replicate the mechanical properties of native skin and provide adequate oxygen and nutrient support remains a formidable challenge. In this study, we incorporated phosphosilicate calcium bioglasses (PSCs), a type of bioactiv...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423003599 |
| _version_ | 1797355234010333184 |
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| author | Yanyan Liu Xin Liu Haitao Guo Xinhuan Wang Ailing Li Dong Qiu Qi Gu |
| author_facet | Yanyan Liu Xin Liu Haitao Guo Xinhuan Wang Ailing Li Dong Qiu Qi Gu |
| author_sort | Yanyan Liu |
| collection | DOAJ |
| description | Constructing three-dimensional (3D) bioprinted skin tissues that accurately replicate the mechanical properties of native skin and provide adequate oxygen and nutrient support remains a formidable challenge. In this study, we incorporated phosphosilicate calcium bioglasses (PSCs), a type of bioactive glass (BG), into the bioinks used for 3D bioprinting. The resulting bioink exhibited mechanical properties and biocompatibility that closely resembled those of natural skin. Utilizing 3D bioprinting technology, we successfully fabricated full-thickness skin substitutes, which underwent comprehensive evaluation to assess their regenerative potential in treating full-thickness skin injuries in rats. Remarkably, the skin substitutes loaded with PSCs exhibited exceptional angiogenic activity, as evidenced by the upregulation of angiogenesis-related genes in vitro and the observation of enhanced vascularization in wound tissue sections in vivo. These findings conclusively demonstrated the outstanding efficacy of PSCs in promoting angiogenesis and facilitating the repair of full-thickness skin wounds. The insights garnered from this study provide a valuable reference strategy for the development of skin tissue grafts with potent angiogenesis-inducing capabilities. |
| first_indexed | 2024-03-08T14:08:19Z |
| format | Article |
| id | doaj.art-b26098753d484636b689f1faa5c3f9fd |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-08T14:08:19Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-b26098753d484636b689f1faa5c3f9fd2024-01-15T04:23:57ZengElsevierMaterials Today Bio2590-00642024-02-01241008993D bioprinting bioglass to construct vascularized full-thickness skin substitutes for wound healingYanyan Liu0Xin Liu1Haitao Guo2Xinhuan Wang3Ailing Li4Dong Qiu5Qi Gu6State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, 100101, PR China; School of Materials Design and Engineering, Beijing Institute of Fashion Technology, Chaoyang District, Beijing, 100029, PR ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Chaoyang District, Beijing, 100101, PR China; Corresponding author. Beijing Institute for Stem Cell and Regenerative Medicine, Chaoyang District, Beijing, 100101, PR China.State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Chaoyang District, Beijing, 100101, PR China; University of Chinese Academy of Sciences, Huairou District, Beijing, 101449, PR ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Chaoyang District, Beijing, 100101, PR ChinaBeijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Haidian District, Beijing, 100190, PR ChinaBeijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Haidian District, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Huairou District, Beijing, 101449, PR ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Chaoyang District, Beijing, 100101, PR China; University of Chinese Academy of Sciences, Huairou District, Beijing, 101449, PR China; Corresponding author. State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, 100101, PR China.Constructing three-dimensional (3D) bioprinted skin tissues that accurately replicate the mechanical properties of native skin and provide adequate oxygen and nutrient support remains a formidable challenge. In this study, we incorporated phosphosilicate calcium bioglasses (PSCs), a type of bioactive glass (BG), into the bioinks used for 3D bioprinting. The resulting bioink exhibited mechanical properties and biocompatibility that closely resembled those of natural skin. Utilizing 3D bioprinting technology, we successfully fabricated full-thickness skin substitutes, which underwent comprehensive evaluation to assess their regenerative potential in treating full-thickness skin injuries in rats. Remarkably, the skin substitutes loaded with PSCs exhibited exceptional angiogenic activity, as evidenced by the upregulation of angiogenesis-related genes in vitro and the observation of enhanced vascularization in wound tissue sections in vivo. These findings conclusively demonstrated the outstanding efficacy of PSCs in promoting angiogenesis and facilitating the repair of full-thickness skin wounds. The insights garnered from this study provide a valuable reference strategy for the development of skin tissue grafts with potent angiogenesis-inducing capabilities.http://www.sciencedirect.com/science/article/pii/S25900064230035993D printingArtificial skin substitutesWound healingAngiogenesisBioactive glass |
| spellingShingle | Yanyan Liu Xin Liu Haitao Guo Xinhuan Wang Ailing Li Dong Qiu Qi Gu 3D bioprinting bioglass to construct vascularized full-thickness skin substitutes for wound healing Materials Today Bio 3D printing Artificial skin substitutes Wound healing Angiogenesis Bioactive glass |
| title | 3D bioprinting bioglass to construct vascularized full-thickness skin substitutes for wound healing |
| title_full | 3D bioprinting bioglass to construct vascularized full-thickness skin substitutes for wound healing |
| title_fullStr | 3D bioprinting bioglass to construct vascularized full-thickness skin substitutes for wound healing |
| title_full_unstemmed | 3D bioprinting bioglass to construct vascularized full-thickness skin substitutes for wound healing |
| title_short | 3D bioprinting bioglass to construct vascularized full-thickness skin substitutes for wound healing |
| title_sort | 3d bioprinting bioglass to construct vascularized full thickness skin substitutes for wound healing |
| topic | 3D printing Artificial skin substitutes Wound healing Angiogenesis Bioactive glass |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423003599 |
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