Microscopic study of edema in hydatidiform mole
Objectives: the purpose of this study is to use light microscopy and scanning electron microscopy to determine the effect of edema on the structure of the molar vesicle. Methods: samples were taken from the complete hydatidiform mole and processed using conventional light and scanning electron micro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Instituto Materno Infantil de Pernambuco
2014-09-01
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| Series: | Revista Brasileira de Saúde Materno Infantil |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-38292014000300261&lng=en&tlng=en |
| _version_ | 1828176679797784576 |
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| author | Olivar C. Castejón Aury Caraballo Oliver Castejón Elizabeth Cedeño |
| author_facet | Olivar C. Castejón Aury Caraballo Oliver Castejón Elizabeth Cedeño |
| author_sort | Olivar C. Castejón |
| collection | DOAJ |
| description | Objectives: the purpose of this study is to use light microscopy and scanning electron microscopy to determine the effect of edema on the structure of the molar vesicle. Methods: samples were taken from the complete hydatidiform mole and processed using conventional light and scanning electron microscopy techniques and an observation protocol that identified four variables: factors underlying the development of edema; the condition of the trophoblast basement membrane, development of the villi, accumulation and degeneration of sulphated mucosubstances at stromal level. Results: light microscopy showed a permeable trophoblastic basement membrane, a swollen syncytium, edematous regions disorganizating the stromal region and causing ischemic necrosis of cells. Using scanning electron microscopy, the basement membrane was found to be distended and thickened, with large irregular holes for the entry and movement of liquid, leaving a wide range of fluids during the influx process and depriving stromal cells of nutrition. Conclusions: a new three-dimensional view of the changes brought about by the entry of fluids into the stroma of molar hydropic vesicles was provided by scanning electron microscopy and confirmed by light microscopy, thereby explaining the changes occurring at the level of the stroma as an effect of the edema. |
| first_indexed | 2024-04-12T04:48:20Z |
| format | Article |
| id | doaj.art-b262e371cbe34f738418255645e02283 |
| institution | Directory Open Access Journal |
| issn | 1806-9304 |
| language | English |
| last_indexed | 2024-04-12T04:48:20Z |
| publishDate | 2014-09-01 |
| publisher | Instituto Materno Infantil de Pernambuco |
| record_format | Article |
| series | Revista Brasileira de Saúde Materno Infantil |
| spelling | doaj.art-b262e371cbe34f738418255645e022832022-12-22T03:47:24ZengInstituto Materno Infantil de PernambucoRevista Brasileira de Saúde Materno Infantil1806-93042014-09-0114326126810.1590/S1519-38292014000300007S1519-38292014000300261Microscopic study of edema in hydatidiform moleOlivar C. CastejónAury CaraballoOliver CastejónElizabeth CedeñoObjectives: the purpose of this study is to use light microscopy and scanning electron microscopy to determine the effect of edema on the structure of the molar vesicle. Methods: samples were taken from the complete hydatidiform mole and processed using conventional light and scanning electron microscopy techniques and an observation protocol that identified four variables: factors underlying the development of edema; the condition of the trophoblast basement membrane, development of the villi, accumulation and degeneration of sulphated mucosubstances at stromal level. Results: light microscopy showed a permeable trophoblastic basement membrane, a swollen syncytium, edematous regions disorganizating the stromal region and causing ischemic necrosis of cells. Using scanning electron microscopy, the basement membrane was found to be distended and thickened, with large irregular holes for the entry and movement of liquid, leaving a wide range of fluids during the influx process and depriving stromal cells of nutrition. Conclusions: a new three-dimensional view of the changes brought about by the entry of fluids into the stroma of molar hydropic vesicles was provided by scanning electron microscopy and confirmed by light microscopy, thereby explaining the changes occurring at the level of the stroma as an effect of the edema.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-38292014000300261&lng=en&tlng=enMola hidatiformeEdemaMicroscopiaMicroscopia eletrônica de varredura |
| spellingShingle | Olivar C. Castejón Aury Caraballo Oliver Castejón Elizabeth Cedeño Microscopic study of edema in hydatidiform mole Revista Brasileira de Saúde Materno Infantil Mola hidatiforme Edema Microscopia Microscopia eletrônica de varredura |
| title | Microscopic study of edema in hydatidiform mole |
| title_full | Microscopic study of edema in hydatidiform mole |
| title_fullStr | Microscopic study of edema in hydatidiform mole |
| title_full_unstemmed | Microscopic study of edema in hydatidiform mole |
| title_short | Microscopic study of edema in hydatidiform mole |
| title_sort | microscopic study of edema in hydatidiform mole |
| topic | Mola hidatiforme Edema Microscopia Microscopia eletrônica de varredura |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-38292014000300261&lng=en&tlng=en |
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