Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E
In this work, we have developed a simple and rapid colorimetric assay for the detection of immunoglobulin E (IgE) using functional nucleic acids (FNAs) and a solid-phase competition enzyme-linked immunosorbent assay (ELISA). The FNAs including aptamer of recombinant IgE, G-quadruplex and its complem...
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MDPI AG
2019-05-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/19/10/2224 |
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| author | Xuexia Lin Caiyun Yu Honggui Lin Cui Wang Jianlong Su Jie Cheng Ranjith Kumar Kankala Shu-Feng Zhou |
| author_facet | Xuexia Lin Caiyun Yu Honggui Lin Cui Wang Jianlong Su Jie Cheng Ranjith Kumar Kankala Shu-Feng Zhou |
| author_sort | Xuexia Lin |
| collection | DOAJ |
| description | In this work, we have developed a simple and rapid colorimetric assay for the detection of immunoglobulin E (IgE) using functional nucleic acids (FNAs) and a solid-phase competition enzyme-linked immunosorbent assay (ELISA). The FNAs including aptamer of recombinant IgE, G-quadruplex and its complementary fragments were immobilized on 96-well microplates to achieve recognition and detection of IgE in biological samples. The G-quadruplex DNAzyme catalyzed 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-hemin-H<sub>2</sub>O<sub>2</sub> system was used to improve the sensitivity of colorimetric assay. In the presence of IgE, the hairpin structure and G-quadruplex would be destroyed, resulting in the inactivation of DNAzyme and subsequent reduction of its absorbance. This cost-effective approach detected IgE in the linear range from 5.0 pg/mL to 500 ng/mL, with the limit of detection (LOD) of 2.0 pg/mL, under optimal conditions. Moreover, the developed method was successfully applied to the rapid detection of IgE in human urine, indicating a great potentiality of this approach in clinical diagnosis and other biomedical applications. |
| first_indexed | 2024-04-11T12:45:22Z |
| format | Article |
| id | doaj.art-b26436c9cebc42bd9c22c4b138e9c2c5 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:45:22Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-b26436c9cebc42bd9c22c4b138e9c2c52022-12-22T04:23:22ZengMDPI AGSensors1424-82202019-05-011910222410.3390/s19102224s19102224Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin EXuexia Lin0Caiyun Yu1Honggui Lin2Cui Wang3Jianlong Su4Jie Cheng5Ranjith Kumar Kankala6Shu-Feng Zhou7Department of Chemical Engineering & Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaDepartment of Chemical Engineering & Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaSchool of Marine Engineering, Jimei University, Xiamen 361021 ChinaApplied and Environment Microbiology, Department of Biology, Georgie State University, Atlanta, GA 30303, USADepartment of Chemical Engineering & Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaDepartment of Chemical Engineering & Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaDepartment of Chemical Engineering & Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaDepartment of Chemical Engineering & Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaIn this work, we have developed a simple and rapid colorimetric assay for the detection of immunoglobulin E (IgE) using functional nucleic acids (FNAs) and a solid-phase competition enzyme-linked immunosorbent assay (ELISA). The FNAs including aptamer of recombinant IgE, G-quadruplex and its complementary fragments were immobilized on 96-well microplates to achieve recognition and detection of IgE in biological samples. The G-quadruplex DNAzyme catalyzed 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-hemin-H<sub>2</sub>O<sub>2</sub> system was used to improve the sensitivity of colorimetric assay. In the presence of IgE, the hairpin structure and G-quadruplex would be destroyed, resulting in the inactivation of DNAzyme and subsequent reduction of its absorbance. This cost-effective approach detected IgE in the linear range from 5.0 pg/mL to 500 ng/mL, with the limit of detection (LOD) of 2.0 pg/mL, under optimal conditions. Moreover, the developed method was successfully applied to the rapid detection of IgE in human urine, indicating a great potentiality of this approach in clinical diagnosis and other biomedical applications.https://www.mdpi.com/1424-8220/19/10/2224functional nucleic acidsDNAzymeimmunoglobulin Ecolorimetric competition detection |
| spellingShingle | Xuexia Lin Caiyun Yu Honggui Lin Cui Wang Jianlong Su Jie Cheng Ranjith Kumar Kankala Shu-Feng Zhou Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E Sensors functional nucleic acids DNAzyme immunoglobulin E colorimetric competition detection |
| title | Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E |
| title_full | Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E |
| title_fullStr | Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E |
| title_full_unstemmed | Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E |
| title_short | Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E |
| title_sort | self assembly of functional nucleic acid based colorimetric competition assay for the detection of immunoglobulin e |
| topic | functional nucleic acids DNAzyme immunoglobulin E colorimetric competition detection |
| url | https://www.mdpi.com/1424-8220/19/10/2224 |
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