Anti-Inflammatory and Neuroprotective Effects of Morin in an MPTP-Induced Parkinson’s Disease Model
Neurodegenerative diseases such as Parkinson’s disease (PD) are known to be related to oxidative stress and neuroinflammation, and thus, modulating neuroinflammation offers a possible means of treating PD-associated pathologies. Morin (2′,3,4′,5,7-pentahydroxy flavone) is a flavonol with anti-oxidat...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/18/10578 |
| _version_ | 1797487256764678144 |
|---|---|
| author | Dong Geun Hong Seulah Lee Jaehoon Kim Seonguk Yang Myunggyo Lee Jinsook Ahn Haeseung Lee Seung-Cheol Chang Nam-Chul Ha Jaewon Lee |
| author_facet | Dong Geun Hong Seulah Lee Jaehoon Kim Seonguk Yang Myunggyo Lee Jinsook Ahn Haeseung Lee Seung-Cheol Chang Nam-Chul Ha Jaewon Lee |
| author_sort | Dong Geun Hong |
| collection | DOAJ |
| description | Neurodegenerative diseases such as Parkinson’s disease (PD) are known to be related to oxidative stress and neuroinflammation, and thus, modulating neuroinflammation offers a possible means of treating PD-associated pathologies. Morin (2′,3,4′,5,7-pentahydroxy flavone) is a flavonol with anti-oxidative and anti-inflammatory effects found in wines, herbs, and fruits. The present study was undertaken to determine whether a morin-containing diet has protective effects in an MPTP-induced mouse model of PD. Mice were fed a control or morin diet for 34 days, and then MPTP (30 mg/kg, i.p.) was administered daily for 5 days to induce a PD-like pathology. We found that dietary morin prevented MPTP-induced motor dysfunction and ameliorated dopaminergic neuronal damage in striatum (STR) and substantia nigra (SN) in our mouse model. Furthermore, MPTP-induced neuroinflammation was significantly reduced in mice fed morin. In vitro studies showed that morin effectively suppressed glial activations in primary microglia and astrocytes, and biochemical analysis and a docking simulation indicated that the anti-inflammatory effects of morin were mediated by blocking the extracellular signal-regulated kinase (ERK)-p65 pathway. These findings suggest that morin effectively inhibits glial activations and has potential use as a functional food ingredient with therapeutic potential for the treatment of PD and other neurodegenerative diseases associated with neuroinflammation. |
| first_indexed | 2024-03-09T23:46:03Z |
| format | Article |
| id | doaj.art-b264399fc5844f6bb50d9dd92227ec66 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:46:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b264399fc5844f6bb50d9dd92227ec662023-11-23T16:44:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181057810.3390/ijms231810578Anti-Inflammatory and Neuroprotective Effects of Morin in an MPTP-Induced Parkinson’s Disease ModelDong Geun Hong0Seulah Lee1Jaehoon Kim2Seonguk Yang3Myunggyo Lee4Jinsook Ahn5Haeseung Lee6Seung-Cheol Chang7Nam-Chul Ha8Jaewon Lee9Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaCenter for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, KoreaCenter for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaNeurodegenerative diseases such as Parkinson’s disease (PD) are known to be related to oxidative stress and neuroinflammation, and thus, modulating neuroinflammation offers a possible means of treating PD-associated pathologies. Morin (2′,3,4′,5,7-pentahydroxy flavone) is a flavonol with anti-oxidative and anti-inflammatory effects found in wines, herbs, and fruits. The present study was undertaken to determine whether a morin-containing diet has protective effects in an MPTP-induced mouse model of PD. Mice were fed a control or morin diet for 34 days, and then MPTP (30 mg/kg, i.p.) was administered daily for 5 days to induce a PD-like pathology. We found that dietary morin prevented MPTP-induced motor dysfunction and ameliorated dopaminergic neuronal damage in striatum (STR) and substantia nigra (SN) in our mouse model. Furthermore, MPTP-induced neuroinflammation was significantly reduced in mice fed morin. In vitro studies showed that morin effectively suppressed glial activations in primary microglia and astrocytes, and biochemical analysis and a docking simulation indicated that the anti-inflammatory effects of morin were mediated by blocking the extracellular signal-regulated kinase (ERK)-p65 pathway. These findings suggest that morin effectively inhibits glial activations and has potential use as a functional food ingredient with therapeutic potential for the treatment of PD and other neurodegenerative diseases associated with neuroinflammation.https://www.mdpi.com/1422-0067/23/18/10578anti-inflammationastrocytemicrogliamorinneuroprotectionParkinson’s disease |
| spellingShingle | Dong Geun Hong Seulah Lee Jaehoon Kim Seonguk Yang Myunggyo Lee Jinsook Ahn Haeseung Lee Seung-Cheol Chang Nam-Chul Ha Jaewon Lee Anti-Inflammatory and Neuroprotective Effects of Morin in an MPTP-Induced Parkinson’s Disease Model International Journal of Molecular Sciences anti-inflammation astrocyte microglia morin neuroprotection Parkinson’s disease |
| title | Anti-Inflammatory and Neuroprotective Effects of Morin in an MPTP-Induced Parkinson’s Disease Model |
| title_full | Anti-Inflammatory and Neuroprotective Effects of Morin in an MPTP-Induced Parkinson’s Disease Model |
| title_fullStr | Anti-Inflammatory and Neuroprotective Effects of Morin in an MPTP-Induced Parkinson’s Disease Model |
| title_full_unstemmed | Anti-Inflammatory and Neuroprotective Effects of Morin in an MPTP-Induced Parkinson’s Disease Model |
| title_short | Anti-Inflammatory and Neuroprotective Effects of Morin in an MPTP-Induced Parkinson’s Disease Model |
| title_sort | anti inflammatory and neuroprotective effects of morin in an mptp induced parkinson s disease model |
| topic | anti-inflammation astrocyte microglia morin neuroprotection Parkinson’s disease |
| url | https://www.mdpi.com/1422-0067/23/18/10578 |
| work_keys_str_mv | AT donggeunhong antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT seulahlee antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT jaehoonkim antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT seongukyang antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT myunggyolee antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT jinsookahn antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT haeseunglee antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT seungcheolchang antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT namchulha antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel AT jaewonlee antiinflammatoryandneuroprotectiveeffectsofmorininanmptpinducedparkinsonsdiseasemodel |