l-Tetrahydropalmatine, an active component of Corydalis yanhusuo W.T. Wang, protects against myocardial ischaemia-reperfusion injury in rats.
l-Tetrahydropalmatine (l-THP) is an active ingredients of Corydalis yanhusuo W.T. Wang, which protects against acute global cerebral ischaemia-reperfusion injury. In this study, we show that l-THP is cardioprotective in myocardial ischaemia-reperfusion injury and examined the mechanism. Rats were tr...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3371051?pdf=render |
| _version_ | 1828485663147687936 |
|---|---|
| author | Yi Han Wen Zhang Yan Tang Wenli Bai Fan Yang Liping Xie Xiaozhen Li Suming Zhou Shiyang Pan Qi Chen Albert Ferro Yong Ji |
| author_facet | Yi Han Wen Zhang Yan Tang Wenli Bai Fan Yang Liping Xie Xiaozhen Li Suming Zhou Shiyang Pan Qi Chen Albert Ferro Yong Ji |
| author_sort | Yi Han |
| collection | DOAJ |
| description | l-Tetrahydropalmatine (l-THP) is an active ingredients of Corydalis yanhusuo W.T. Wang, which protects against acute global cerebral ischaemia-reperfusion injury. In this study, we show that l-THP is cardioprotective in myocardial ischaemia-reperfusion injury and examined the mechanism. Rats were treated with l-THP (0, 10, 20, 40 mg/kg b.w.) for 20 min before occlusion of the left anterior descending coronary artery and subjected to myocardial ischaemia-reperfusion (30 min/6 h). Compared with vehicle-treated animals, the infarct area/risk area (IA/RA) of l-THP (20, 40 mg/kg b.w.) treated rats was reduced, whilst l-THP (10 mg/kg b.w.) had no significant effect. Cardiac function was improved in l-THP-treated rats whilst plasma creatine kinase activity declined. Following treatment with l-THP (20 mg/kg b.w.), subunit of phosphatidylinositol 3-kinase p85, serine(473) phosphorylation of Akt and serine(1177) phosphorylation of endothelial NO synthase (eNOS) increased in myocardium, whilst expression of inducible NO synthase (iNOS) decreased. However, the expression of HIF-1α and VEGF were increased in I(30 min)R(6 h), but decreased to normal level in I(30 min)R(24 h), while treatment with l-THP (20 mg/kg b.w.) enhanced the levels of these two genes in I(30 min)R(24 h). Production of NO in myocardium and plasma, activity of myeloperoxidase (MPO) in plasma and the expression of tumour necrosis factor-α (TNF-α) in myocardium were decreased by l-THP. TUNEL assay revealed that l-THP (20 mg/kg b.w.) reduced apoptosis in myocardium. Thus, we show that l-THP activates the PI3K/Akt/eNOS/NO pathway and increases expression of HIF-1α and VEGF, whilst depressing iNOS-derived NO production in myocardium. This effect may decrease the accumulation of inflammatory factors, including TNF-α and MPO, and lessen the extent of apoptosis, therefore contributing to the cardioprotective effects of l-THP in myocardial ischaemia-reperfusion injury. |
| first_indexed | 2024-12-11T09:18:54Z |
| format | Article |
| id | doaj.art-b2693d6118224d7db396b93d3028a656 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T09:18:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b2693d6118224d7db396b93d3028a6562022-12-22T01:13:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3862710.1371/journal.pone.0038627l-Tetrahydropalmatine, an active component of Corydalis yanhusuo W.T. Wang, protects against myocardial ischaemia-reperfusion injury in rats.Yi HanWen ZhangYan TangWenli BaiFan YangLiping XieXiaozhen LiSuming ZhouShiyang PanQi ChenAlbert FerroYong Jil-Tetrahydropalmatine (l-THP) is an active ingredients of Corydalis yanhusuo W.T. Wang, which protects against acute global cerebral ischaemia-reperfusion injury. In this study, we show that l-THP is cardioprotective in myocardial ischaemia-reperfusion injury and examined the mechanism. Rats were treated with l-THP (0, 10, 20, 40 mg/kg b.w.) for 20 min before occlusion of the left anterior descending coronary artery and subjected to myocardial ischaemia-reperfusion (30 min/6 h). Compared with vehicle-treated animals, the infarct area/risk area (IA/RA) of l-THP (20, 40 mg/kg b.w.) treated rats was reduced, whilst l-THP (10 mg/kg b.w.) had no significant effect. Cardiac function was improved in l-THP-treated rats whilst plasma creatine kinase activity declined. Following treatment with l-THP (20 mg/kg b.w.), subunit of phosphatidylinositol 3-kinase p85, serine(473) phosphorylation of Akt and serine(1177) phosphorylation of endothelial NO synthase (eNOS) increased in myocardium, whilst expression of inducible NO synthase (iNOS) decreased. However, the expression of HIF-1α and VEGF were increased in I(30 min)R(6 h), but decreased to normal level in I(30 min)R(24 h), while treatment with l-THP (20 mg/kg b.w.) enhanced the levels of these two genes in I(30 min)R(24 h). Production of NO in myocardium and plasma, activity of myeloperoxidase (MPO) in plasma and the expression of tumour necrosis factor-α (TNF-α) in myocardium were decreased by l-THP. TUNEL assay revealed that l-THP (20 mg/kg b.w.) reduced apoptosis in myocardium. Thus, we show that l-THP activates the PI3K/Akt/eNOS/NO pathway and increases expression of HIF-1α and VEGF, whilst depressing iNOS-derived NO production in myocardium. This effect may decrease the accumulation of inflammatory factors, including TNF-α and MPO, and lessen the extent of apoptosis, therefore contributing to the cardioprotective effects of l-THP in myocardial ischaemia-reperfusion injury.http://europepmc.org/articles/PMC3371051?pdf=render |
| spellingShingle | Yi Han Wen Zhang Yan Tang Wenli Bai Fan Yang Liping Xie Xiaozhen Li Suming Zhou Shiyang Pan Qi Chen Albert Ferro Yong Ji l-Tetrahydropalmatine, an active component of Corydalis yanhusuo W.T. Wang, protects against myocardial ischaemia-reperfusion injury in rats. PLoS ONE |
| title | l-Tetrahydropalmatine, an active component of Corydalis yanhusuo W.T. Wang, protects against myocardial ischaemia-reperfusion injury in rats. |
| title_full | l-Tetrahydropalmatine, an active component of Corydalis yanhusuo W.T. Wang, protects against myocardial ischaemia-reperfusion injury in rats. |
| title_fullStr | l-Tetrahydropalmatine, an active component of Corydalis yanhusuo W.T. Wang, protects against myocardial ischaemia-reperfusion injury in rats. |
| title_full_unstemmed | l-Tetrahydropalmatine, an active component of Corydalis yanhusuo W.T. Wang, protects against myocardial ischaemia-reperfusion injury in rats. |
| title_short | l-Tetrahydropalmatine, an active component of Corydalis yanhusuo W.T. Wang, protects against myocardial ischaemia-reperfusion injury in rats. |
| title_sort | l tetrahydropalmatine an active component of corydalis yanhusuo w t wang protects against myocardial ischaemia reperfusion injury in rats |
| url | http://europepmc.org/articles/PMC3371051?pdf=render |
| work_keys_str_mv | AT yihan ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT wenzhang ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT yantang ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT wenlibai ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT fanyang ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT lipingxie ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT xiaozhenli ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT sumingzhou ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT shiyangpan ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT qichen ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT albertferro ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats AT yongji ltetrahydropalmatineanactivecomponentofcorydalisyanhusuowtwangprotectsagainstmyocardialischaemiareperfusioninjuryinrats |