The <it>Adhesion </it>GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury

<p>Abstract</p> <p>Background</p> <p>GPR125 belongs to the family of <it>Adhesion </it>G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a <it>Drosophila </it>sequence, DmCG15744...

Full description

Bibliographic Details
Main Authors: Fredriksson Robert, Chodobski Adam, Waller Linn, Palha Joana A, Marques Fernanda, Szmydynger-Chodobska Joanna, Hägglund Maria, Pickering Chris, Lagerström Malin C, Schiöth Helgi B
Format: Article
Language:English
Published: BMC 2008-10-01
Series:BMC Neuroscience
Online Access:http://www.biomedcentral.com/1471-2202/9/97
_version_ 1818393583523201024
author Fredriksson Robert
Chodobski Adam
Waller Linn
Palha Joana A
Marques Fernanda
Szmydynger-Chodobska Joanna
Hägglund Maria
Pickering Chris
Lagerström Malin C
Schiöth Helgi B
author_facet Fredriksson Robert
Chodobski Adam
Waller Linn
Palha Joana A
Marques Fernanda
Szmydynger-Chodobska Joanna
Hägglund Maria
Pickering Chris
Lagerström Malin C
Schiöth Helgi B
author_sort Fredriksson Robert
collection DOAJ
description <p>Abstract</p> <p>Background</p> <p>GPR125 belongs to the family of <it>Adhesion </it>G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a <it>Drosophila </it>sequence, DmCG15744, which shares a common ancestor with the entire Group III of <it>Adhesio</it>n GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.</p> <p>Results</p> <p>We found specific expression of GPR125 in cells of the choroid plexus using <it>in situ </it>hybridization and protein-specific antibodies and combined <it>in situ</it>/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.</p> <p>Conclusion</p> <p>These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.</p>
first_indexed 2024-12-14T05:47:37Z
format Article
id doaj.art-b26a7221d45f446a8cfb6ca0b977e093
institution Directory Open Access Journal
issn 1471-2202
language English
last_indexed 2024-12-14T05:47:37Z
publishDate 2008-10-01
publisher BMC
record_format Article
series BMC Neuroscience
spelling doaj.art-b26a7221d45f446a8cfb6ca0b977e0932022-12-21T23:14:49ZengBMCBMC Neuroscience1471-22022008-10-01919710.1186/1471-2202-9-97The <it>Adhesion </it>GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injuryFredriksson RobertChodobski AdamWaller LinnPalha Joana AMarques FernandaSzmydynger-Chodobska JoannaHägglund MariaPickering ChrisLagerström Malin CSchiöth Helgi B<p>Abstract</p> <p>Background</p> <p>GPR125 belongs to the family of <it>Adhesion </it>G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a <it>Drosophila </it>sequence, DmCG15744, which shares a common ancestor with the entire Group III of <it>Adhesio</it>n GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.</p> <p>Results</p> <p>We found specific expression of GPR125 in cells of the choroid plexus using <it>in situ </it>hybridization and protein-specific antibodies and combined <it>in situ</it>/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury.</p> <p>Conclusion</p> <p>These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.</p>http://www.biomedcentral.com/1471-2202/9/97
spellingShingle Fredriksson Robert
Chodobski Adam
Waller Linn
Palha Joana A
Marques Fernanda
Szmydynger-Chodobska Joanna
Hägglund Maria
Pickering Chris
Lagerström Malin C
Schiöth Helgi B
The <it>Adhesion </it>GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury
BMC Neuroscience
title The <it>Adhesion </it>GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury
title_full The <it>Adhesion </it>GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury
title_fullStr The <it>Adhesion </it>GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury
title_full_unstemmed The <it>Adhesion </it>GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury
title_short The <it>Adhesion </it>GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury
title_sort it adhesion it gpcr gpr125 is specifically expressed in the choroid plexus and is upregulated following brain injury
url http://www.biomedcentral.com/1471-2202/9/97
work_keys_str_mv AT fredrikssonrobert theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT chodobskiadam theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT wallerlinn theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT palhajoanaa theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT marquesfernanda theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT szmydyngerchodobskajoanna theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT hagglundmaria theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT pickeringchris theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT lagerstrommalinc theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT schiothhelgib theitadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT fredrikssonrobert itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT chodobskiadam itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT wallerlinn itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT palhajoanaa itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT marquesfernanda itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT szmydyngerchodobskajoanna itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT hagglundmaria itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT pickeringchris itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT lagerstrommalinc itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury
AT schiothhelgib itadhesionitgpcrgpr125isspecificallyexpressedinthechoroidplexusandisupregulatedfollowingbraininjury