Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization
Propolis is a resinous vegetal exudate modified by bees, and is interesting as a preservative and potentially functional product. This work dealt with studying the common phenolic profiles and antioxidant capacities of 13 bee propolis from different geographical areas. Both hyaluronidase and angiote...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/1/75 |
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| author | Sandra M. Osés Patricia Marcos Patricia Azofra Ana de Pablo Miguel Ángel Fernández-Muíño M. Teresa Sancho |
| author_facet | Sandra M. Osés Patricia Marcos Patricia Azofra Ana de Pablo Miguel Ángel Fernández-Muíño M. Teresa Sancho |
| author_sort | Sandra M. Osés |
| collection | DOAJ |
| description | Propolis is a resinous vegetal exudate modified by bees, and is interesting as a preservative and potentially functional product. This work dealt with studying the common phenolic profiles and antioxidant capacities of 13 bee propolis from different geographical areas. Both hyaluronidase and angiotensin converting enzyme (ACE) inhibitory activities were also assessed and related when possible with particular phenolic compounds. High performance liquid chromatography-ultraviolet detection (HPLC-UV) analysis showed that every propolis contained <i>p</i>-coumaric acid (1.2−12.2 mg/g) and ferulic acid (0.3−11.0 mg/g). Pinocembrin, catechin, and caffeic acid phenethyl ester (CAPE) plus galangin were the main flavonoids. Antioxidant activities were higher than 280 µmol trolox/g for trolox equivalent antioxidant capacity (TEAC), 0.099 mmol uric acid/g for radical-scavenging effect on hydroxyl radicals, and 0.19 mg/mL for half maximal inhibitory concentration (IC<sub>50</sub>) of antioxidant activity against superoxide anion radical. Working with solutions of 10 mg/mL propolis, hyaluronidase inhibitory activity ranged between 0% and 68.20%, being correlated to ferulic acid content. ACE inhibitory effect determined by HPLC was higher than 78%, being correlated with catechin and <i>p</i>-coumaric acid. Therefore, propolis could be useful for food, pharmaceutical, and cosmetic companies, also helping to reduce risk factors for diseases related to oxidative damage, inflammatory processes, and hypertension. This research also highlights the necessity for harmonized analysis methods and the expression of results for propolis. |
| first_indexed | 2024-03-12T20:22:43Z |
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| id | doaj.art-b26b50ed6b8640909e4f5dc4a5c3e131 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-12T20:22:43Z |
| publishDate | 2020-01-01 |
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| series | Antioxidants |
| spelling | doaj.art-b26b50ed6b8640909e4f5dc4a5c3e1312023-08-02T00:45:42ZengMDPI AGAntioxidants2076-39212020-01-01917510.3390/antiox9010075antiox9010075Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical HarmonizationSandra M. Osés0Patricia Marcos1Patricia Azofra2Ana de Pablo3Miguel Ángel Fernández-Muíño4M. Teresa Sancho5Department of Biotechnology and Food Science, Universidad de Burgos (University of Burgos), Pza. Misael Bañuelos s/n, 09001 Burgos, SpainDepartment of Biotechnology and Food Science, Universidad de Burgos (University of Burgos), Pza. Misael Bañuelos s/n, 09001 Burgos, SpainDepartment of Biotechnology and Food Science, Universidad de Burgos (University of Burgos), Pza. Misael Bañuelos s/n, 09001 Burgos, SpainDepartment of Biotechnology and Food Science, Universidad de Burgos (University of Burgos), Pza. Misael Bañuelos s/n, 09001 Burgos, SpainDepartment of Biotechnology and Food Science, Universidad de Burgos (University of Burgos), Pza. Misael Bañuelos s/n, 09001 Burgos, SpainDepartment of Biotechnology and Food Science, Universidad de Burgos (University of Burgos), Pza. Misael Bañuelos s/n, 09001 Burgos, SpainPropolis is a resinous vegetal exudate modified by bees, and is interesting as a preservative and potentially functional product. This work dealt with studying the common phenolic profiles and antioxidant capacities of 13 bee propolis from different geographical areas. Both hyaluronidase and angiotensin converting enzyme (ACE) inhibitory activities were also assessed and related when possible with particular phenolic compounds. High performance liquid chromatography-ultraviolet detection (HPLC-UV) analysis showed that every propolis contained <i>p</i>-coumaric acid (1.2−12.2 mg/g) and ferulic acid (0.3−11.0 mg/g). Pinocembrin, catechin, and caffeic acid phenethyl ester (CAPE) plus galangin were the main flavonoids. Antioxidant activities were higher than 280 µmol trolox/g for trolox equivalent antioxidant capacity (TEAC), 0.099 mmol uric acid/g for radical-scavenging effect on hydroxyl radicals, and 0.19 mg/mL for half maximal inhibitory concentration (IC<sub>50</sub>) of antioxidant activity against superoxide anion radical. Working with solutions of 10 mg/mL propolis, hyaluronidase inhibitory activity ranged between 0% and 68.20%, being correlated to ferulic acid content. ACE inhibitory effect determined by HPLC was higher than 78%, being correlated with catechin and <i>p</i>-coumaric acid. Therefore, propolis could be useful for food, pharmaceutical, and cosmetic companies, also helping to reduce risk factors for diseases related to oxidative damage, inflammatory processes, and hypertension. This research also highlights the necessity for harmonized analysis methods and the expression of results for propolis.https://www.mdpi.com/2076-3921/9/1/75propolisphenolic profileantioxidant capacitieshyaluronidase inhibitory activityace inhibitory activity |
| spellingShingle | Sandra M. Osés Patricia Marcos Patricia Azofra Ana de Pablo Miguel Ángel Fernández-Muíño M. Teresa Sancho Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization Antioxidants propolis phenolic profile antioxidant capacities hyaluronidase inhibitory activity ace inhibitory activity |
| title | Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization |
| title_full | Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization |
| title_fullStr | Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization |
| title_full_unstemmed | Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization |
| title_short | Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization |
| title_sort | phenolic profile antioxidant capacities and enzymatic inhibitory activities of propolis from different geographical areas needs for analytical harmonization |
| topic | propolis phenolic profile antioxidant capacities hyaluronidase inhibitory activity ace inhibitory activity |
| url | https://www.mdpi.com/2076-3921/9/1/75 |
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