Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study)
The ultrastructure of the tracheal epithelium in rabbits exposed for 96 hours to 35-37% O2 was studied in our experiments. Due to the influence of mild normobaric hyperoxia, massive differentiation of new secretory elements was initiated and resulted in apparent changes in goblet cells distribution....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2003-11-01
|
| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200311-0002_mild-hyperoxia-induces-moderate-pathological-alteration-in-airway-epithelium-ultrastructural-study.php |
| _version_ | 1828006941666836480 |
|---|---|
| author | V. Koonradova J. Uhlik L. Vajner J. Herget J. Adaskova |
| author_facet | V. Koonradova J. Uhlik L. Vajner J. Herget J. Adaskova |
| author_sort | V. Koonradova |
| collection | DOAJ |
| description | The ultrastructure of the tracheal epithelium in rabbits exposed for 96 hours to 35-37% O2 was studied in our experiments. Due to the influence of mild normobaric hyperoxia, massive differentiation of new secretory elements was initiated and resulted in apparent changes in goblet cells distribution. 60 ± 4% of goblet cells took part in the formation of voluminous intraepithelial mucous glands. Ciliated cells were less damaged than the goblet ones. Tiny signs of pathological alteration of deeper portions of their cytoplasm and apical blebbing accompanied with destruction of some kinocilia were encountered. The ciliary border was slightly impaired. Mild, but significant decrease in the mean number of kinocilia/μm2 went along with significant increase in percentage of altered cilia. Among the altered kinocilia, the slightly altered pathological cilia with local swellings of the ciliary membranes or with tiny vacuoles situated in their shafts were the most numerous. Hyperoxia did not influence the process of ciliogenesis in the ciliated cells. As morphological signs of impairment of the vital self-cleaning ability of the airway epithelium, layers of inspissated mucus were encountered in the area of the ciliary border. From morphological point of view, mild hyperoxia caused moderate damage to the airway epithelium. |
| first_indexed | 2024-04-10T08:03:15Z |
| format | Article |
| id | doaj.art-b26dac3ac0e248bf8e15d2747bee8dac |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:03:15Z |
| publishDate | 2003-11-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-b26dac3ac0e248bf8e15d2747bee8dac2023-02-23T03:49:03ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922003-11-01481131332010.17221/5785-VETMEDvet-200311-0002Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study)V. Koonradova0J. Uhlik1L. Vajner2J. Herget3J. Adaskova4, J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic, J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic, J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic, J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic, J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech RepublicThe ultrastructure of the tracheal epithelium in rabbits exposed for 96 hours to 35-37% O2 was studied in our experiments. Due to the influence of mild normobaric hyperoxia, massive differentiation of new secretory elements was initiated and resulted in apparent changes in goblet cells distribution. 60 ± 4% of goblet cells took part in the formation of voluminous intraepithelial mucous glands. Ciliated cells were less damaged than the goblet ones. Tiny signs of pathological alteration of deeper portions of their cytoplasm and apical blebbing accompanied with destruction of some kinocilia were encountered. The ciliary border was slightly impaired. Mild, but significant decrease in the mean number of kinocilia/μm2 went along with significant increase in percentage of altered cilia. Among the altered kinocilia, the slightly altered pathological cilia with local swellings of the ciliary membranes or with tiny vacuoles situated in their shafts were the most numerous. Hyperoxia did not influence the process of ciliogenesis in the ciliated cells. As morphological signs of impairment of the vital self-cleaning ability of the airway epithelium, layers of inspissated mucus were encountered in the area of the ciliary border. From morphological point of view, mild hyperoxia caused moderate damage to the airway epithelium.https://vetmed.agriculturejournals.cz/artkey/vet-200311-0002_mild-hyperoxia-induces-moderate-pathological-alteration-in-airway-epithelium-ultrastructural-study.phptracheaoxygenultrastructurerabbit |
| spellingShingle | V. Koonradova J. Uhlik L. Vajner J. Herget J. Adaskova Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study) Veterinární Medicína trachea oxygen ultrastructure rabbit |
| title | Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study) |
| title_full | Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study) |
| title_fullStr | Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study) |
| title_full_unstemmed | Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study) |
| title_short | Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study) |
| title_sort | mild hyperoxia induces moderate pathological alteration in airway epithelium ultrastructural study |
| topic | trachea oxygen ultrastructure rabbit |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200311-0002_mild-hyperoxia-induces-moderate-pathological-alteration-in-airway-epithelium-ultrastructural-study.php |
| work_keys_str_mv | AT vkoonradova mildhyperoxiainducesmoderatepathologicalalterationinairwayepitheliumultrastructuralstudy AT juhlik mildhyperoxiainducesmoderatepathologicalalterationinairwayepitheliumultrastructuralstudy AT lvajner mildhyperoxiainducesmoderatepathologicalalterationinairwayepitheliumultrastructuralstudy AT jherget mildhyperoxiainducesmoderatepathologicalalterationinairwayepitheliumultrastructuralstudy AT jadaskova mildhyperoxiainducesmoderatepathologicalalterationinairwayepitheliumultrastructuralstudy |