Preclinical PET and MR Evaluation of <sup>89</sup>Zr- and <sup>68</sup>Ga-Labeled Nanodiamonds in Mice over Different Time Scales
Nanodiamonds (NDs) have high potential as a drug carrier and in combination with nitrogen vacancies (NV centers) for highly sensitive MR-imaging after hyperpolarization. However, little remains known about their physiological properties in vivo. PET imaging allows further evaluation due to its quant...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/24/4471 |
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| author | Gordon Winter Nina Eberhardt Jessica Löffler Marco Raabe Md. Noor A. Alam Li Hao Alireza Abaei Hendrik Herrmann Claudia Kuntner Gerhard Glatting Christoph Solbach Fedor Jelezko Tanja Weil Ambros J. Beer Volker Rasche |
| author_facet | Gordon Winter Nina Eberhardt Jessica Löffler Marco Raabe Md. Noor A. Alam Li Hao Alireza Abaei Hendrik Herrmann Claudia Kuntner Gerhard Glatting Christoph Solbach Fedor Jelezko Tanja Weil Ambros J. Beer Volker Rasche |
| author_sort | Gordon Winter |
| collection | DOAJ |
| description | Nanodiamonds (NDs) have high potential as a drug carrier and in combination with nitrogen vacancies (NV centers) for highly sensitive MR-imaging after hyperpolarization. However, little remains known about their physiological properties in vivo. PET imaging allows further evaluation due to its quantitative properties and high sensitivity. Thus, we aimed to create a preclinical platform for PET and MR evaluation of surface-modified NDs by radiolabeling with both short- and long-lived radiotracers. Serum albumin coated NDs, functionalized with PEG groups and the chelator deferoxamine, were labeled either with zirconium-89 or gallium-68. Their biodistribution was assessed in two different mouse strains. PET scans were performed at various time points up to 7 d after i.v. injection. Anatomical correlation was provided by additional MRI in a subset of animals. PET results were validated by ex vivo quantification of the excised organs using a gamma counter. Radiolabeled NDs accumulated rapidly in the liver and spleen with a slight increase over time, while rapid washout from the blood pool was observed. Significant differences between the investigated radionuclides were only observed for the spleen (1 h). In summary, we successfully created a preclinical PET and MR imaging platform for the evaluation of the biodistribution of NDs over different time scales. |
| first_indexed | 2024-03-09T16:01:34Z |
| format | Article |
| id | doaj.art-b26f5ea8e3c447788141c7b3eeb08342 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T16:01:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-b26f5ea8e3c447788141c7b3eeb083422023-11-24T17:04:57ZengMDPI AGNanomaterials2079-49912022-12-011224447110.3390/nano12244471Preclinical PET and MR Evaluation of <sup>89</sup>Zr- and <sup>68</sup>Ga-Labeled Nanodiamonds in Mice over Different Time ScalesGordon Winter0Nina Eberhardt1Jessica Löffler2Marco Raabe3Md. Noor A. Alam4Li Hao5Alireza Abaei6Hendrik Herrmann7Claudia Kuntner8Gerhard Glatting9Christoph Solbach10Fedor Jelezko11Tanja Weil12Ambros J. Beer13Volker Rasche14Department of Nuclear Medicine, Ulm University Medical Center, 89081 Ulm, GermanyDepartment of Nuclear Medicine, Ulm University Medical Center, 89081 Ulm, GermanyDepartment of Nuclear Medicine, Ulm University Medical Center, 89081 Ulm, GermanyDepartment of Synthesis of Macromolecules, Max Planck Institute for Polymer Research, 55128 Mainz, GermanyDepartment of Synthesis of Macromolecules, Max Planck Institute for Polymer Research, 55128 Mainz, GermanyDepartment of Internal Medicine II, Experimental Cardiovascular Imaging, Ulm University Medical Center, 89081 Ulm, GermanyDepartment of Internal Medicine II, Experimental Cardiovascular Imaging, Ulm University Medical Center, 89081 Ulm, GermanyDepartment of Nuclear Medicine, Ulm University Medical Center, 89081 Ulm, GermanyDepartment of Biomedical Imaging and Image-Guided Therapy, Medical University Vienna, 1090 Vienna, AustriaDepartment of Nuclear Medicine, Ulm University Medical Center, 89081 Ulm, GermanyDepartment of Nuclear Medicine, Ulm University Medical Center, 89081 Ulm, GermanyInstitute for Quantum Optics, Ulm University, 89081 Ulm, GermanyDepartment of Synthesis of Macromolecules, Max Planck Institute for Polymer Research, 55128 Mainz, GermanyDepartment of Nuclear Medicine, Ulm University Medical Center, 89081 Ulm, GermanyDepartment of Internal Medicine II, Experimental Cardiovascular Imaging, Ulm University Medical Center, 89081 Ulm, GermanyNanodiamonds (NDs) have high potential as a drug carrier and in combination with nitrogen vacancies (NV centers) for highly sensitive MR-imaging after hyperpolarization. However, little remains known about their physiological properties in vivo. PET imaging allows further evaluation due to its quantitative properties and high sensitivity. Thus, we aimed to create a preclinical platform for PET and MR evaluation of surface-modified NDs by radiolabeling with both short- and long-lived radiotracers. Serum albumin coated NDs, functionalized with PEG groups and the chelator deferoxamine, were labeled either with zirconium-89 or gallium-68. Their biodistribution was assessed in two different mouse strains. PET scans were performed at various time points up to 7 d after i.v. injection. Anatomical correlation was provided by additional MRI in a subset of animals. PET results were validated by ex vivo quantification of the excised organs using a gamma counter. Radiolabeled NDs accumulated rapidly in the liver and spleen with a slight increase over time, while rapid washout from the blood pool was observed. Significant differences between the investigated radionuclides were only observed for the spleen (1 h). In summary, we successfully created a preclinical PET and MR imaging platform for the evaluation of the biodistribution of NDs over different time scales.https://www.mdpi.com/2079-4991/12/24/4471nanodiamondsPET imagingMRI imagingbiodistributionnanoparticles<sup>89</sup>Zr |
| spellingShingle | Gordon Winter Nina Eberhardt Jessica Löffler Marco Raabe Md. Noor A. Alam Li Hao Alireza Abaei Hendrik Herrmann Claudia Kuntner Gerhard Glatting Christoph Solbach Fedor Jelezko Tanja Weil Ambros J. Beer Volker Rasche Preclinical PET and MR Evaluation of <sup>89</sup>Zr- and <sup>68</sup>Ga-Labeled Nanodiamonds in Mice over Different Time Scales Nanomaterials nanodiamonds PET imaging MRI imaging biodistribution nanoparticles <sup>89</sup>Zr |
| title | Preclinical PET and MR Evaluation of <sup>89</sup>Zr- and <sup>68</sup>Ga-Labeled Nanodiamonds in Mice over Different Time Scales |
| title_full | Preclinical PET and MR Evaluation of <sup>89</sup>Zr- and <sup>68</sup>Ga-Labeled Nanodiamonds in Mice over Different Time Scales |
| title_fullStr | Preclinical PET and MR Evaluation of <sup>89</sup>Zr- and <sup>68</sup>Ga-Labeled Nanodiamonds in Mice over Different Time Scales |
| title_full_unstemmed | Preclinical PET and MR Evaluation of <sup>89</sup>Zr- and <sup>68</sup>Ga-Labeled Nanodiamonds in Mice over Different Time Scales |
| title_short | Preclinical PET and MR Evaluation of <sup>89</sup>Zr- and <sup>68</sup>Ga-Labeled Nanodiamonds in Mice over Different Time Scales |
| title_sort | preclinical pet and mr evaluation of sup 89 sup zr and sup 68 sup ga labeled nanodiamonds in mice over different time scales |
| topic | nanodiamonds PET imaging MRI imaging biodistribution nanoparticles <sup>89</sup>Zr |
| url | https://www.mdpi.com/2079-4991/12/24/4471 |
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