Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay
Tulbaghia violacea (wild garlic) is commonly used in traditional medicine for the treatment of various ailments including fungal infections, gastrointestinal ailments, asthma, fever, colds and pulmonary tuberculosis. We assessed the potential genotoxic effects of water extracts from the leaves, stem...
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| Format: | Article |
| Language: | English |
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Academy of Science of South Africa
2019-01-01
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| Series: | South African Journal of Science |
| Subjects: | |
| Online Access: | https://www.sajs.co.za/article/view/4391 |
| _version_ | 1828414333029187584 |
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| author | Lerato N. Madike Samkeliso Takaidza Cornelius Ssemakalu Michael Pillay |
| author_facet | Lerato N. Madike Samkeliso Takaidza Cornelius Ssemakalu Michael Pillay |
| author_sort | Lerato N. Madike |
| collection | DOAJ |
| description | Tulbaghia violacea (wild garlic) is commonly used in traditional medicine for the treatment of various ailments including fungal infections, gastrointestinal ailments, asthma, fever, colds and pulmonary tuberculosis. We assessed the potential genotoxic effects of water extracts from the leaves, stems and roots of T. violacea using the Allium cepa assay. Extracts at concentrations of 100, 250, 500 and 1000 μg/mL were tested on root meristems of A. cepa. Ethidium bromide was used as a positive control whereas distilled water acted as a negative control. The results reveal that as the concentrations of the water extracts of T. violacea increased, the mitotic indices decreased. Similarly, the percentage of chromosomal aberrations was dependent on the concentration as well as on which part of the plant was used. The six most common chromosome aberrations included laggard chromosomes, chromosome bridges, c-mitosis, sticky chromosomes, formation of binuclei and formation of trinuclei. The presence of micronucleated cells at interphase also increased as the concentration of the water extracts increased. The results confirm that water extracts of T. violacea exert significant genotoxic effects at higher concentrations, with the stem extracts being more toxic than the leaf and root extracts at similar concentrations.
Significance:
• Water extracts of T. violacea – a plant commonly used in traditional medicine – were found to have significant genotoxic effects at higher concentrations. |
| first_indexed | 2024-12-10T13:24:44Z |
| format | Article |
| id | doaj.art-b26fcf8ada5b421d82e8574a3df959da |
| institution | Directory Open Access Journal |
| issn | 1996-7489 |
| language | English |
| last_indexed | 2024-12-10T13:24:44Z |
| publishDate | 2019-01-01 |
| publisher | Academy of Science of South Africa |
| record_format | Article |
| series | South African Journal of Science |
| spelling | doaj.art-b26fcf8ada5b421d82e8574a3df959da2022-12-22T01:47:14ZengAcademy of Science of South AfricaSouth African Journal of Science1996-74892019-01-011151/210.17159/sajs.2019/43914391Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assayLerato N. Madike0Samkeliso Takaidza1Cornelius Ssemakalu2Michael Pillay3Department of Biotechnology, Vaal University of Technology, Vanderbijlpark, South AfricaDepartment of Biotechnology, Vaal University of Technology, Vanderbijlpark, South AfricaDepartment of Biotechnology, Vaal University of Technology, Vanderbijlpark, South AfricaDepartment of Biotechnology, Vaal University of Technology, Vanderbijlpark, South AfricaTulbaghia violacea (wild garlic) is commonly used in traditional medicine for the treatment of various ailments including fungal infections, gastrointestinal ailments, asthma, fever, colds and pulmonary tuberculosis. We assessed the potential genotoxic effects of water extracts from the leaves, stems and roots of T. violacea using the Allium cepa assay. Extracts at concentrations of 100, 250, 500 and 1000 μg/mL were tested on root meristems of A. cepa. Ethidium bromide was used as a positive control whereas distilled water acted as a negative control. The results reveal that as the concentrations of the water extracts of T. violacea increased, the mitotic indices decreased. Similarly, the percentage of chromosomal aberrations was dependent on the concentration as well as on which part of the plant was used. The six most common chromosome aberrations included laggard chromosomes, chromosome bridges, c-mitosis, sticky chromosomes, formation of binuclei and formation of trinuclei. The presence of micronucleated cells at interphase also increased as the concentration of the water extracts increased. The results confirm that water extracts of T. violacea exert significant genotoxic effects at higher concentrations, with the stem extracts being more toxic than the leaf and root extracts at similar concentrations. Significance: • Water extracts of T. violacea – a plant commonly used in traditional medicine – were found to have significant genotoxic effects at higher concentrations.https://www.sajs.co.za/article/view/4391traditional medicineschromosome aberrationsethidium bromidegenotoxicmitotic index |
| spellingShingle | Lerato N. Madike Samkeliso Takaidza Cornelius Ssemakalu Michael Pillay Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay South African Journal of Science traditional medicines chromosome aberrations ethidium bromide genotoxic mitotic index |
| title | Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay |
| title_full | Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay |
| title_fullStr | Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay |
| title_full_unstemmed | Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay |
| title_short | Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay |
| title_sort | genotoxicity of aqueous extracts of tulbaghia violacea as determined through an allium cepa assay |
| topic | traditional medicines chromosome aberrations ethidium bromide genotoxic mitotic index |
| url | https://www.sajs.co.za/article/view/4391 |
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