Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease
Abstract Background The role of adiponectin (ADIPOQ) in Alzheimer’s disease (AD) has been documented, however, demonstrating controversial results. In this study, we investigated blood serum ADIPOQ levels, methylation of the adiponectin gene promoter, and adiponectin receptors (AdipoR1 and AdipoR2)...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Medical Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12920-022-01420-8 |
| _version_ | 1811291713209106432 |
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| author | Aiym Kaiyrlykyzy Bauyrzhan Umbayev Abdul-Razak Masoud Aida Baibulatova Andrey Tsoy Farkhad Olzhayev Dinara Alzhanova Gulnaz Zholdasbekova Kairat Davletov Ainur Akilzhanova Sholpan Askarova |
| author_facet | Aiym Kaiyrlykyzy Bauyrzhan Umbayev Abdul-Razak Masoud Aida Baibulatova Andrey Tsoy Farkhad Olzhayev Dinara Alzhanova Gulnaz Zholdasbekova Kairat Davletov Ainur Akilzhanova Sholpan Askarova |
| author_sort | Aiym Kaiyrlykyzy |
| collection | DOAJ |
| description | Abstract Background The role of adiponectin (ADIPOQ) in Alzheimer’s disease (AD) has been documented, however, demonstrating controversial results. In this study, we investigated blood serum ADIPOQ levels, methylation of the adiponectin gene promoter, and adiponectin receptors (AdipoR1 and AdipoR2) expression in blood samples isolated from AD patients and healthy controls. Methods We performed a case–control study including 248 subjects (98 AD patients and 150 healthy controls); ADIPOQ serum levels, AdipoR1, and AdipoR2 levels in PBMC were measured by ELISA Kits, and ADIPOQ gene methylation was analyzed using methyl-specific PCR. Results Serum adiponectin levels were threefold higher in the AD group compared to the controls. We have also found a positive correlation between adiponectin and MMSE scores and high-density lipoprotein cholesterol (HDL-C) in AD patients. A significant difference in the proportion of methylation of the CpG sites at − 74 nt of the ADIPOQ gene promoter was detected in AD cases, and the levels of adiponectin in blood serum were significantly higher in methylated samples in the AD group compared to controls. The amount of AdipoR1 was significantly higher among AD subjects, while the expression of AdipoR2 did not vary between AD patients and controls. Conclusion These findings may contribute to a deeper understanding of the etiological factors leading to the development of dementia and may serve as a basis for the development of predictive biomarkers of AD. |
| first_indexed | 2024-04-13T04:33:27Z |
| format | Article |
| id | doaj.art-b27127b660f2440595948987f0fdd10d |
| institution | Directory Open Access Journal |
| issn | 1755-8794 |
| language | English |
| last_indexed | 2024-04-13T04:33:27Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genomics |
| spelling | doaj.art-b27127b660f2440595948987f0fdd10d2022-12-22T03:02:14ZengBMCBMC Medical Genomics1755-87942022-12-0115111010.1186/s12920-022-01420-8Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s diseaseAiym Kaiyrlykyzy0Bauyrzhan Umbayev1Abdul-Razak Masoud2Aida Baibulatova3Andrey Tsoy4Farkhad Olzhayev5Dinara Alzhanova6Gulnaz Zholdasbekova7Kairat Davletov8Ainur Akilzhanova9Sholpan Askarova10Center for Life Sciences, National Laboratory Astana, Nazarbayev UniversityCenter for Life Sciences, National Laboratory Astana, Nazarbayev UniversityCenter for Life Sciences, National Laboratory Astana, Nazarbayev UniversityCenter for Life Sciences, National Laboratory Astana, Nazarbayev UniversityCenter for Life Sciences, National Laboratory Astana, Nazarbayev UniversityCenter for Life Sciences, National Laboratory Astana, Nazarbayev UniversityDepartment of Neurology and Psychiatry, Astana Medical UniversityKaraganda State Medical UniversityAsfendiyarov Kazakh National Medical UniversityCenter for Life Sciences, National Laboratory Astana, Nazarbayev UniversityCenter for Life Sciences, National Laboratory Astana, Nazarbayev UniversityAbstract Background The role of adiponectin (ADIPOQ) in Alzheimer’s disease (AD) has been documented, however, demonstrating controversial results. In this study, we investigated blood serum ADIPOQ levels, methylation of the adiponectin gene promoter, and adiponectin receptors (AdipoR1 and AdipoR2) expression in blood samples isolated from AD patients and healthy controls. Methods We performed a case–control study including 248 subjects (98 AD patients and 150 healthy controls); ADIPOQ serum levels, AdipoR1, and AdipoR2 levels in PBMC were measured by ELISA Kits, and ADIPOQ gene methylation was analyzed using methyl-specific PCR. Results Serum adiponectin levels were threefold higher in the AD group compared to the controls. We have also found a positive correlation between adiponectin and MMSE scores and high-density lipoprotein cholesterol (HDL-C) in AD patients. A significant difference in the proportion of methylation of the CpG sites at − 74 nt of the ADIPOQ gene promoter was detected in AD cases, and the levels of adiponectin in blood serum were significantly higher in methylated samples in the AD group compared to controls. The amount of AdipoR1 was significantly higher among AD subjects, while the expression of AdipoR2 did not vary between AD patients and controls. Conclusion These findings may contribute to a deeper understanding of the etiological factors leading to the development of dementia and may serve as a basis for the development of predictive biomarkers of AD.https://doi.org/10.1186/s12920-022-01420-8Alzheimer’s diseaseAdiponectinAdipoR1AdipoR2DNA methylation |
| spellingShingle | Aiym Kaiyrlykyzy Bauyrzhan Umbayev Abdul-Razak Masoud Aida Baibulatova Andrey Tsoy Farkhad Olzhayev Dinara Alzhanova Gulnaz Zholdasbekova Kairat Davletov Ainur Akilzhanova Sholpan Askarova Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease BMC Medical Genomics Alzheimer’s disease Adiponectin AdipoR1 AdipoR2 DNA methylation |
| title | Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease |
| title_full | Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease |
| title_fullStr | Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease |
| title_full_unstemmed | Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease |
| title_short | Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease |
| title_sort | circulating adiponectin levels expression of adiponectin receptors and methylation of adiponectin gene promoter in relation to alzheimer s disease |
| topic | Alzheimer’s disease Adiponectin AdipoR1 AdipoR2 DNA methylation |
| url | https://doi.org/10.1186/s12920-022-01420-8 |
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