Effect of whey powder and physical form of feed on humoral immune response and expression of interleukin-4 and interferon-gamma transcripts in jejunum of broiler chickens
This study was conducted to assess the effect of whey powder and physical form of feed on humoral immunity and transcript abundances of interleukin-4(IL-4) and interferon-gamma (In-γ) genes on 240 Ross-308 day-old-chickens. The experiment was conducted in a completely randomized design in 6 treatmen...
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2018-09-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
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| Online Access: | https://jab.uk.ac.ir/article_2149_cfe0fc042fb1443fb9f5ff5a44d83fa1.pdf |
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| author | sonia zakizadeh Maryam Torabi Ali Javadmanesh Ali Zanganeh Mohammad-Hossien Nazem-Shirazi |
| author_facet | sonia zakizadeh Maryam Torabi Ali Javadmanesh Ali Zanganeh Mohammad-Hossien Nazem-Shirazi |
| author_sort | sonia zakizadeh |
| collection | DOAJ |
| description | This study was conducted to assess the effect of whey powder and physical form of feed on humoral immunity and transcript abundances of interleukin-4(IL-4) and interferon-gamma (In-γ) genes on 240 Ross-308 day-old-chickens. The experiment was conducted in a completely randomized design in 6 treatments in factorial arrangement (2×3), whey powder at three levels (0, 4,8%) and physical form in two levels (mash, pellet) in broiler chicken diet with 4 repeats and 10 chickens per replicate. The diets formulated based on nutritional requirements of Ross strain-308 (2009) and were fed to the birds in three periods. To determine the immunity titer of ND, IBV, IBD and influenza blood samples were collected at day 10, 31 and 42. At the end of periods, one chicken was selected from experimental units and slaughtered; lymphatic organs were weighted and gene expression of IL-4 and In-γ measured after mRNA extraction and cDNA synthesis. It was found that the addition of whey powder did not have a significant effect on the weights of the spleen and bursa of Fabricius, but the pelleting in the growing stage had a significant effect on the weight of spleen (p<0.01). Pelleting and adding whey to diet in the growing period increased the IL-4 gene expression (p<0.05). In-γ expression was not influenced by whey powder, but pelleting increased its expression during the starter (p<0.01) and grower (p<0.01) stages. |
| first_indexed | 2024-04-13T13:33:58Z |
| format | Article |
| id | doaj.art-b271dde251f54f0eb88cecfb384874d4 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-04-13T13:33:58Z |
| publishDate | 2018-09-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-b271dde251f54f0eb88cecfb384874d42022-12-22T02:44:51ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002018-09-01102194210.22103/jab.2018.21492149Effect of whey powder and physical form of feed on humoral immune response and expression of interleukin-4 and interferon-gamma transcripts in jejunum of broiler chickenssonia zakizadeh0Maryam Torabi1Ali Javadmanesh2Ali Zanganeh3Mohammad-Hossien Nazem-Shirazi4Animal genetics and breeding- Animal Science Research Institute (ASRI)Ph.D. Biotechnologist, Molecular Biology Laboratory of Veterinary Organization of Khorasan Razavi. Mashhad, Iran.Assistant Professor, Departent of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran.Animal Science and Veterinary Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran.DVM and International Laboratory Specialist of World Bank project, Kabul, Afghanistan; DVM and Former International Laboratory Specialist of FAO project, Kabul, AfghanistanThis study was conducted to assess the effect of whey powder and physical form of feed on humoral immunity and transcript abundances of interleukin-4(IL-4) and interferon-gamma (In-γ) genes on 240 Ross-308 day-old-chickens. The experiment was conducted in a completely randomized design in 6 treatments in factorial arrangement (2×3), whey powder at three levels (0, 4,8%) and physical form in two levels (mash, pellet) in broiler chicken diet with 4 repeats and 10 chickens per replicate. The diets formulated based on nutritional requirements of Ross strain-308 (2009) and were fed to the birds in three periods. To determine the immunity titer of ND, IBV, IBD and influenza blood samples were collected at day 10, 31 and 42. At the end of periods, one chicken was selected from experimental units and slaughtered; lymphatic organs were weighted and gene expression of IL-4 and In-γ measured after mRNA extraction and cDNA synthesis. It was found that the addition of whey powder did not have a significant effect on the weights of the spleen and bursa of Fabricius, but the pelleting in the growing stage had a significant effect on the weight of spleen (p<0.01). Pelleting and adding whey to diet in the growing period increased the IL-4 gene expression (p<0.05). In-γ expression was not influenced by whey powder, but pelleting increased its expression during the starter (p<0.01) and grower (p<0.01) stages.https://jab.uk.ac.ir/article_2149_cfe0fc042fb1443fb9f5ff5a44d83fa1.pdflymphoid organshumoral immunitygene expressionvaccine titration |
| spellingShingle | sonia zakizadeh Maryam Torabi Ali Javadmanesh Ali Zanganeh Mohammad-Hossien Nazem-Shirazi Effect of whey powder and physical form of feed on humoral immune response and expression of interleukin-4 and interferon-gamma transcripts in jejunum of broiler chickens مجله بیوتکنولوژی کشاورزی lymphoid organs humoral immunity gene expression vaccine titration |
| title | Effect of whey powder and physical form of feed on humoral immune response and expression of interleukin-4 and interferon-gamma transcripts in jejunum of broiler chickens |
| title_full | Effect of whey powder and physical form of feed on humoral immune response and expression of interleukin-4 and interferon-gamma transcripts in jejunum of broiler chickens |
| title_fullStr | Effect of whey powder and physical form of feed on humoral immune response and expression of interleukin-4 and interferon-gamma transcripts in jejunum of broiler chickens |
| title_full_unstemmed | Effect of whey powder and physical form of feed on humoral immune response and expression of interleukin-4 and interferon-gamma transcripts in jejunum of broiler chickens |
| title_short | Effect of whey powder and physical form of feed on humoral immune response and expression of interleukin-4 and interferon-gamma transcripts in jejunum of broiler chickens |
| title_sort | effect of whey powder and physical form of feed on humoral immune response and expression of interleukin 4 and interferon gamma transcripts in jejunum of broiler chickens |
| topic | lymphoid organs humoral immunity gene expression vaccine titration |
| url | https://jab.uk.ac.ir/article_2149_cfe0fc042fb1443fb9f5ff5a44d83fa1.pdf |
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