Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus
Fungal spores in the air can be inhaled and enter the human respiratory tract. The entry of fungi into the respiratory tract can cause colonization or infection depending on the host immune response. Fungal colonization is the first step into debilitating fungal disease in humans, especially in imm...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2023-08-01
|
| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/39463 |
| _version_ | 1797689947313930240 |
|---|---|
| author | Jamal Zaini Abul A'la Al Maududi Zahrah Annisa Denny Grecius Siregar Findra Setianingrum Mulyati Tugiran Ridhawati Sjam Robiatul Adawiyah Anna Rozaliyani Sita Andarini Elisna Syahruddin |
| author_facet | Jamal Zaini Abul A'la Al Maududi Zahrah Annisa Denny Grecius Siregar Findra Setianingrum Mulyati Tugiran Ridhawati Sjam Robiatul Adawiyah Anna Rozaliyani Sita Andarini Elisna Syahruddin |
| author_sort | Jamal Zaini |
| collection | DOAJ |
| description |
Fungal spores in the air can be inhaled and enter the human respiratory tract. The entry of fungi into the respiratory tract can cause colonization or infection depending on the host immune response. Fungal colonization is the first step into debilitating fungal disease in humans, especially in immunocompromised groups. The increased rate of drug-resistant fungi has been reported in human disease and the environment. This study aims to examine the diversity of fungal colonization in humans and the rate of fungal resistance to voriconazole. This cross-sectional study was done in patients with naïve lung cancer who had not been previously treated with any cancer therapy nor given antifungal agent. Induced sputum from 70 subjects was collected and inoculated in the Sabouraud Dextrose Agar medium. Macroscopic and microscopic examinations were performed to identify fungal species. Voriconazole susceptibility tests were done using the disc diffusion method. This study found Candida albicans, Aspergillus niger, Aspergillus fumigatus, and Penicillium sp. among the most common lower respiratory tract colonies. This study also found the colonization of up to 5 species in a single subject. A high rate of voriconazole-resistant Aspergillus sp. was found (42.4%) among 59 isolates tested. Given that these subjects had never taken antifungal agents previously, the high rate of voriconazole resistance might be attributed to the environment, such as community and agriculture. Mitigation of antifungal use in the agricultural sector, fungal diversity in the environment, and clinical study of fungal colonization/ infection in other high-risk groups are needed.
|
| first_indexed | 2024-03-12T01:53:35Z |
| format | Article |
| id | doaj.art-b272bfe662794f5f9b760bc4775a2a57 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-03-12T01:53:35Z |
| publishDate | 2023-08-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-b272bfe662794f5f9b760bc4775a2a572023-09-08T08:39:36ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942023-08-0130610.4308/hjb.30.6.1139-1148Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant AspergillusJamal Zaini0Abul A'la Al Maududi 1Zahrah Annisa2Denny Grecius Siregar3Findra Setianingrum4Mulyati Tugiran5Ridhawati Sjam6Robiatul Adawiyah7Anna Rozaliyani8Sita Andarini9Elisna Syahruddin10Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Persahabatan National Respiratory Referral Hospital, Jakarta 13230, IndonesiaDepartment of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Persahabatan National Respiratory Referral Hospital, Jakarta 13230, IndonesiaDepartment of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Persahabatan National Respiratory Referral Hospital, Jakarta 13230, IndonesiaDepartment of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Persahabatan National Respiratory Referral Hospital, Jakarta 13230, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. Indonesia Pulmonary Mycosis Centre, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. Indonesia Pulmonary Mycosis Centre, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. Indonesia Pulmonary Mycosis Centre, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. Indonesia Pulmonary Mycosis Centre, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. Indonesia Pulmonary Mycosis Centre, Jakarta 10430, IndonesiaDepartment of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Persahabatan National Respiratory Referral Hospital, Jakarta 13230, IndonesiaDepartment of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Persahabatan National Respiratory Referral Hospital, Jakarta 13230, Indonesia Fungal spores in the air can be inhaled and enter the human respiratory tract. The entry of fungi into the respiratory tract can cause colonization or infection depending on the host immune response. Fungal colonization is the first step into debilitating fungal disease in humans, especially in immunocompromised groups. The increased rate of drug-resistant fungi has been reported in human disease and the environment. This study aims to examine the diversity of fungal colonization in humans and the rate of fungal resistance to voriconazole. This cross-sectional study was done in patients with naïve lung cancer who had not been previously treated with any cancer therapy nor given antifungal agent. Induced sputum from 70 subjects was collected and inoculated in the Sabouraud Dextrose Agar medium. Macroscopic and microscopic examinations were performed to identify fungal species. Voriconazole susceptibility tests were done using the disc diffusion method. This study found Candida albicans, Aspergillus niger, Aspergillus fumigatus, and Penicillium sp. among the most common lower respiratory tract colonies. This study also found the colonization of up to 5 species in a single subject. A high rate of voriconazole-resistant Aspergillus sp. was found (42.4%) among 59 isolates tested. Given that these subjects had never taken antifungal agents previously, the high rate of voriconazole resistance might be attributed to the environment, such as community and agriculture. Mitigation of antifungal use in the agricultural sector, fungal diversity in the environment, and clinical study of fungal colonization/ infection in other high-risk groups are needed. https://journal.ipb.ac.id/index.php/hayati/article/view/39463 |
| spellingShingle | Jamal Zaini Abul A'la Al Maududi Zahrah Annisa Denny Grecius Siregar Findra Setianingrum Mulyati Tugiran Ridhawati Sjam Robiatul Adawiyah Anna Rozaliyani Sita Andarini Elisna Syahruddin Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus Hayati Journal of Biosciences |
| title | Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus |
| title_full | Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus |
| title_fullStr | Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus |
| title_full_unstemmed | Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus |
| title_short | Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus |
| title_sort | diversity of fungal colonization in respiratory tract of naive lung cancer and the emergence of voriconazole resistant aspergillus |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/39463 |
| work_keys_str_mv | AT jamalzaini diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT abulalaalmaududi diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT zahrahannisa diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT dennygreciussiregar diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT findrasetianingrum diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT mulyatitugiran diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT ridhawatisjam diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT robiatuladawiyah diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT annarozaliyani diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT sitaandarini diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus AT elisnasyahruddin diversityoffungalcolonizationinrespiratorytractofnaivelungcancerandtheemergenceofvoriconazoleresistantaspergillus |