Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model
Abstract Background Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-018-1185-9 |
| _version_ | 1818971572092796928 |
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| author | Huynh Tan Hop Lauren Togonon Arayan Alisha Wehdnesday Bernardo Reyes Tran Xuan Ngoc Huy Won Gi Min Hu Jang Lee Man Hee Rhee Hong Hee Chang Suk Kim |
| author_facet | Huynh Tan Hop Lauren Togonon Arayan Alisha Wehdnesday Bernardo Reyes Tran Xuan Ngoc Huy Won Gi Min Hu Jang Lee Man Hee Rhee Hong Hee Chang Suk Kim |
| author_sort | Huynh Tan Hop |
| collection | DOAJ |
| description | Abstract Background Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumulation and apoptosis in murine macrophages and in mice. Results RAW264.7 cells were incubated at 43 °C, and BALB/c mice were subjected to whole body hyperthermia. The data showed a reduction in bacterial survival in the mice after daily heat exposure. This was accompanied by increased levels of cytokines TNF, IL-6, IL-1β and IFN-γ in the sera of the mice. Gene expression of NF-κB and inducible nitric oxide production were also induced in the mouse splenic cells. In parallel with the bacterial reduction in the mouse model, an increased bactericidal effect was observed in RAW264.7 cells after exposure to heat stress. In addition, the heat stress increased both the nuclear translocation of NF-κB and the expression of the heat shock proteins HSP70 and HSP90 in murine macrophages. Furthermore, heat exposure induced the increase of pro-inflammatory cytokines, ROS accumulation and apoptosis but did not affect the production of nitric oxide (NO) in macrophages. Conclusion This study demonstrated the induction of innate immune responses by heat stress that significantly reduced the intracellular survival of B. abortus in vitro and in vivo. Transcriptional factor NF-κB, which is a master regulator, could be termed a key activator of heat-induced immunity against Brucella. The increase in the expression and activation of NF-κB in splenic cells and macrophages was followed by enhanced antimicrobial effectors, including cytokines, ROS and NO that may contribute to the reduction of bacterial survival. |
| first_indexed | 2024-12-20T14:54:30Z |
| format | Article |
| id | doaj.art-b27475a0db0543f59df56fba55b486d1 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-20T14:54:30Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-b27475a0db0543f59df56fba55b486d12022-12-21T19:36:55ZengBMCBMC Microbiology1471-21802018-05-0118111210.1186/s12866-018-1185-9Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse modelHuynh Tan Hop0Lauren Togonon Arayan1Alisha Wehdnesday Bernardo Reyes2Tran Xuan Ngoc Huy3Won Gi Min4Hu Jang Lee5Man Hee Rhee6Hong Hee Chang7Suk Kim8Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National UniversityInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National UniversityInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National UniversityInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National UniversityInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National UniversityInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National UniversityCollege of Veterinary Medicine, Kyungpook National UniversityInstitute of Agriculture and Life Science, Gyeongsang National UniversityInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National UniversityAbstract Background Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumulation and apoptosis in murine macrophages and in mice. Results RAW264.7 cells were incubated at 43 °C, and BALB/c mice were subjected to whole body hyperthermia. The data showed a reduction in bacterial survival in the mice after daily heat exposure. This was accompanied by increased levels of cytokines TNF, IL-6, IL-1β and IFN-γ in the sera of the mice. Gene expression of NF-κB and inducible nitric oxide production were also induced in the mouse splenic cells. In parallel with the bacterial reduction in the mouse model, an increased bactericidal effect was observed in RAW264.7 cells after exposure to heat stress. In addition, the heat stress increased both the nuclear translocation of NF-κB and the expression of the heat shock proteins HSP70 and HSP90 in murine macrophages. Furthermore, heat exposure induced the increase of pro-inflammatory cytokines, ROS accumulation and apoptosis but did not affect the production of nitric oxide (NO) in macrophages. Conclusion This study demonstrated the induction of innate immune responses by heat stress that significantly reduced the intracellular survival of B. abortus in vitro and in vivo. Transcriptional factor NF-κB, which is a master regulator, could be termed a key activator of heat-induced immunity against Brucella. The increase in the expression and activation of NF-κB in splenic cells and macrophages was followed by enhanced antimicrobial effectors, including cytokines, ROS and NO that may contribute to the reduction of bacterial survival.http://link.springer.com/article/10.1186/s12866-018-1185-9B. abortusHeat stressMacrophageNF-κBROS |
| spellingShingle | Huynh Tan Hop Lauren Togonon Arayan Alisha Wehdnesday Bernardo Reyes Tran Xuan Ngoc Huy Won Gi Min Hu Jang Lee Man Hee Rhee Hong Hee Chang Suk Kim Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model BMC Microbiology B. abortus Heat stress Macrophage NF-κB ROS |
| title | Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model |
| title_full | Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model |
| title_fullStr | Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model |
| title_full_unstemmed | Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model |
| title_short | Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model |
| title_sort | heat stress modulated induction of nf κb leads to brucellacidal pro inflammatory defense against brucella abortus infection in murine macrophages and in a mouse model |
| topic | B. abortus Heat stress Macrophage NF-κB ROS |
| url | http://link.springer.com/article/10.1186/s12866-018-1185-9 |
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