Forabot: Automated Planktic Foraminifera Isolation and Imaging

Abstract Physical inspection and sorting of foraminifera is a necessity in many research labs, as foraminifera serve as paleoenvironmental and chronostratigraphic indicators. In order to gain counts of species from samples, analyze chemical compositions, or extract morphological properties of forami...

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Main Authors: Turner Richmond, Jeremy Cole, Gabriella Dangler, Michael Daniele, Thomas Marchitto, Edgar Lobaton
Format: Article
Language:English
Published: Wiley 2022-12-01
Series:Geochemistry, Geophysics, Geosystems
Subjects:
Online Access:https://doi.org/10.1029/2022GC010689
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author Turner Richmond
Jeremy Cole
Gabriella Dangler
Michael Daniele
Thomas Marchitto
Edgar Lobaton
author_facet Turner Richmond
Jeremy Cole
Gabriella Dangler
Michael Daniele
Thomas Marchitto
Edgar Lobaton
author_sort Turner Richmond
collection DOAJ
description Abstract Physical inspection and sorting of foraminifera is a necessity in many research labs, as foraminifera serve as paleoenvironmental and chronostratigraphic indicators. In order to gain counts of species from samples, analyze chemical compositions, or extract morphological properties of foraminifera, research labs require human time and effort handling and sorting these microscopic fossils. The presented work describes Forabot, an open‐source system which can physically manipulate individual foraminifera for imaging and isolation with minimal human interaction. The major components to build a Forabot are outlined in this work, with supplementary information available which allows for other researchers to build a Forabot with low‐cost, off‐the‐shelf components. From a washed and sieved sample of hundreds of foraminifera, the Forabot is shown to be capable of isolating and imaging individual forams. The timing of the Forabot's current pipeline allows for the processing of up to 27 foram specimens per hour, a rate that can be improved for future classification purposes by reducing image quality and/or quantity. Along with the physical descriptions, the image processing and classification pipelines are also reviewed. A proof‐of‐concept classifier utilizes a finetuned VGG‐16 network to achieve a classification accuracy of 79% on a validation set of foraminifera images collected with Forabot. In conclusion, the system is able to be built by researchers for a low cost, effectively manipulate foraminifera with few mistakes, provide quality images for future research, and classify the species of imaged forams.
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spelling doaj.art-b27495c1118c418283f7460030dddce22023-11-03T17:00:34ZengWileyGeochemistry, Geophysics, Geosystems1525-20272022-12-012312n/an/a10.1029/2022GC010689Forabot: Automated Planktic Foraminifera Isolation and ImagingTurner Richmond0Jeremy Cole1Gabriella Dangler2Michael Daniele3Thomas Marchitto4Edgar Lobaton5Department of Electrical and Computer Engineering North Carolina State University Raleigh NC USADepartment of Electrical and Computer Engineering North Carolina State University Raleigh NC USADepartment of Electrical and Computer Engineering North Carolina State University Raleigh NC USADepartment of Electrical and Computer Engineering North Carolina State University Raleigh NC USAInstitute of Arctic and Alpine Research University of Colorado Boulder CO USADepartment of Electrical and Computer Engineering North Carolina State University Raleigh NC USAAbstract Physical inspection and sorting of foraminifera is a necessity in many research labs, as foraminifera serve as paleoenvironmental and chronostratigraphic indicators. In order to gain counts of species from samples, analyze chemical compositions, or extract morphological properties of foraminifera, research labs require human time and effort handling and sorting these microscopic fossils. The presented work describes Forabot, an open‐source system which can physically manipulate individual foraminifera for imaging and isolation with minimal human interaction. The major components to build a Forabot are outlined in this work, with supplementary information available which allows for other researchers to build a Forabot with low‐cost, off‐the‐shelf components. From a washed and sieved sample of hundreds of foraminifera, the Forabot is shown to be capable of isolating and imaging individual forams. The timing of the Forabot's current pipeline allows for the processing of up to 27 foram specimens per hour, a rate that can be improved for future classification purposes by reducing image quality and/or quantity. Along with the physical descriptions, the image processing and classification pipelines are also reviewed. A proof‐of‐concept classifier utilizes a finetuned VGG‐16 network to achieve a classification accuracy of 79% on a validation set of foraminifera images collected with Forabot. In conclusion, the system is able to be built by researchers for a low cost, effectively manipulate foraminifera with few mistakes, provide quality images for future research, and classify the species of imaged forams.https://doi.org/10.1029/2022GC010689foraminiferaautomationclassification
spellingShingle Turner Richmond
Jeremy Cole
Gabriella Dangler
Michael Daniele
Thomas Marchitto
Edgar Lobaton
Forabot: Automated Planktic Foraminifera Isolation and Imaging
Geochemistry, Geophysics, Geosystems
foraminifera
automation
classification
title Forabot: Automated Planktic Foraminifera Isolation and Imaging
title_full Forabot: Automated Planktic Foraminifera Isolation and Imaging
title_fullStr Forabot: Automated Planktic Foraminifera Isolation and Imaging
title_full_unstemmed Forabot: Automated Planktic Foraminifera Isolation and Imaging
title_short Forabot: Automated Planktic Foraminifera Isolation and Imaging
title_sort forabot automated planktic foraminifera isolation and imaging
topic foraminifera
automation
classification
url https://doi.org/10.1029/2022GC010689
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