Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns
Physical interactions between cells and micro/nanometer-sized architecture presented in an extracellular matrix (ECM) environment significantly influence cell adhesion and morphology, often facilitating the incidence of diseases, such as cancer invasion and metastasis. Sensing and responding to the...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.825970/full |
| _version_ | 1811239974872285184 |
|---|---|
| author | Kyung Ah Kim Srivithya Vellampatti Byoung Choul Kim |
| author_facet | Kyung Ah Kim Srivithya Vellampatti Byoung Choul Kim |
| author_sort | Kyung Ah Kim |
| collection | DOAJ |
| description | Physical interactions between cells and micro/nanometer-sized architecture presented in an extracellular matrix (ECM) environment significantly influence cell adhesion and morphology, often facilitating the incidence of diseases, such as cancer invasion and metastasis. Sensing and responding to the topographical cues are deeply associated with a physical interplay between integrins, ligands, and mechanical force transmission, ultimately determining diverse cell behavior. Thus, how the tension applied to the integrin-ligand bonds controls cells’ response to the topographical cues needs to be elucidated through quantitative analysis. Here, in this brief research report, we reported a novel platform, termed “topo-tension gauge tether (TGT),” to visualize single-molecule force applied to the integrin-ligand on the aligned anisotropic nanopatterns. Using the topo-TGT assay, first, topography-induced adhesion and morphology of cancerous and normal cells were compared with the pre-defined peak integrin tension. Next, spatial integrin tensions underneath cells were identified using reconstructed integrin tension maps. As a result, we characterized each cell’s capability to comply with nanotopographies and the magnitude of the spatial integrin tension. Altogether, the quantitative information on integrin tension will be a valuable basis for understanding the biophysical mechanisms underlying the force balance influencing adhesion to the topographical cues. |
| first_indexed | 2024-04-12T13:11:15Z |
| format | Article |
| id | doaj.art-b274fa674e434084b27db276fdabdc9e |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-12T13:11:15Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-b274fa674e434084b27db276fdabdc9e2022-12-22T03:31:52ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-06-01910.3389/fmolb.2022.825970825970Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic NanopatternsKyung Ah KimSrivithya VellampattiByoung Choul KimPhysical interactions between cells and micro/nanometer-sized architecture presented in an extracellular matrix (ECM) environment significantly influence cell adhesion and morphology, often facilitating the incidence of diseases, such as cancer invasion and metastasis. Sensing and responding to the topographical cues are deeply associated with a physical interplay between integrins, ligands, and mechanical force transmission, ultimately determining diverse cell behavior. Thus, how the tension applied to the integrin-ligand bonds controls cells’ response to the topographical cues needs to be elucidated through quantitative analysis. Here, in this brief research report, we reported a novel platform, termed “topo-tension gauge tether (TGT),” to visualize single-molecule force applied to the integrin-ligand on the aligned anisotropic nanopatterns. Using the topo-TGT assay, first, topography-induced adhesion and morphology of cancerous and normal cells were compared with the pre-defined peak integrin tension. Next, spatial integrin tensions underneath cells were identified using reconstructed integrin tension maps. As a result, we characterized each cell’s capability to comply with nanotopographies and the magnitude of the spatial integrin tension. Altogether, the quantitative information on integrin tension will be a valuable basis for understanding the biophysical mechanisms underlying the force balance influencing adhesion to the topographical cues.https://www.frontiersin.org/articles/10.3389/fmolb.2022.825970/fullmechanobiologyintegrin tensiontension gauge tethermolecular force sensorcanceranisotropic nanopattern |
| spellingShingle | Kyung Ah Kim Srivithya Vellampatti Byoung Choul Kim Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns Frontiers in Molecular Biosciences mechanobiology integrin tension tension gauge tether molecular force sensor cancer anisotropic nanopattern |
| title | Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns |
| title_full | Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns |
| title_fullStr | Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns |
| title_full_unstemmed | Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns |
| title_short | Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns |
| title_sort | characterization of integrin molecular tension of human breast cancer cells on anisotropic nanopatterns |
| topic | mechanobiology integrin tension tension gauge tether molecular force sensor cancer anisotropic nanopattern |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.825970/full |
| work_keys_str_mv | AT kyungahkim characterizationofintegrinmoleculartensionofhumanbreastcancercellsonanisotropicnanopatterns AT srivithyavellampatti characterizationofintegrinmoleculartensionofhumanbreastcancercellsonanisotropicnanopatterns AT byoungchoulkim characterizationofintegrinmoleculartensionofhumanbreastcancercellsonanisotropicnanopatterns |