Fermentative Hydrogen Production from Lignocellulose by Mesophilic <i>Clostridium populeti</i> FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated Compost
Screening new <i>Clostridium</i> strains that can efficiently utilize lignocellulose to produce hydrogen is extremely important for dark fermentative hydrogen production. In this study, a mesophilic hydrogen-producing bacterium, identified as <i>Clostridium populeti</i> FZ10,...
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MDPI AG
2022-09-01
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| author | Jingnan Zhang Baoxuan Jiang Haokun Zhang Sitong Qian Tao Wei Zhiping Zhang Lili Song Xu Yang |
| author_facet | Jingnan Zhang Baoxuan Jiang Haokun Zhang Sitong Qian Tao Wei Zhiping Zhang Lili Song Xu Yang |
| author_sort | Jingnan Zhang |
| collection | DOAJ |
| description | Screening new <i>Clostridium</i> strains that can efficiently utilize lignocellulose to produce hydrogen is extremely important for dark fermentative hydrogen production. In this study, a mesophilic hydrogen-producing bacterium, identified as <i>Clostridium populeti</i> FZ10, was newly isolated from compost acclimated by microcrystalline cellulose. The strain could produce hydrogen from various cellulosic substrates. The performances of hydrogen production from microcrystalline cellulose (MCC) and corn stalk (CS) were especially investigated. The maximum hydrogen yield and hydrogen production rate from MCC were 177.5 ± 4.8 mL/g and 7.7 ± 0.2 mL·g<sup>−1</sup>·h<sup>−1</sup>, respectively. Furthermore, scanning electron microscopy (SEM) images showed that the structure of CS was destroyed after fermentation, which could be attributed to the presence of exoglucanase, endoglucanase, β-glucosidase and xylanase produced by <i>Clostridium populeti</i> FZ10. The maximum hydrogen yield and hydrogen production rate from CS were 92.5 ± 3.7 mL/g and 5.9 ± 0.2 mL·g<sup>−1</sup>·h<sup>−1</sup>,respectively, with a cellulose degradation of 47.2 ± 2.3% and a hemicellulose degradation of 58.1 ± 2.0%. This study demonstrates that <i>Clostridium populeti</i> FZ10 is an ideal candidate for directly converting lignocellulose into biohydrogen under mesophilic conditions. The discovery of strain <i>C</i><i>. populeti</i> FZ10 has special significance in the field of bioenergy. |
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| spelling | doaj.art-b2768b8aa33543a5abbc61d0ac7dde672023-11-23T19:41:43ZengMDPI AGApplied Sciences2076-34172022-09-011219956210.3390/app12199562Fermentative Hydrogen Production from Lignocellulose by Mesophilic <i>Clostridium populeti</i> FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated CompostJingnan Zhang0Baoxuan Jiang1Haokun Zhang2Sitong Qian3Tao Wei4Zhiping Zhang5Lili Song6Xu Yang7College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaScreening new <i>Clostridium</i> strains that can efficiently utilize lignocellulose to produce hydrogen is extremely important for dark fermentative hydrogen production. In this study, a mesophilic hydrogen-producing bacterium, identified as <i>Clostridium populeti</i> FZ10, was newly isolated from compost acclimated by microcrystalline cellulose. The strain could produce hydrogen from various cellulosic substrates. The performances of hydrogen production from microcrystalline cellulose (MCC) and corn stalk (CS) were especially investigated. The maximum hydrogen yield and hydrogen production rate from MCC were 177.5 ± 4.8 mL/g and 7.7 ± 0.2 mL·g<sup>−1</sup>·h<sup>−1</sup>, respectively. Furthermore, scanning electron microscopy (SEM) images showed that the structure of CS was destroyed after fermentation, which could be attributed to the presence of exoglucanase, endoglucanase, β-glucosidase and xylanase produced by <i>Clostridium populeti</i> FZ10. The maximum hydrogen yield and hydrogen production rate from CS were 92.5 ± 3.7 mL/g and 5.9 ± 0.2 mL·g<sup>−1</sup>·h<sup>−1</sup>,respectively, with a cellulose degradation of 47.2 ± 2.3% and a hemicellulose degradation of 58.1 ± 2.0%. This study demonstrates that <i>Clostridium populeti</i> FZ10 is an ideal candidate for directly converting lignocellulose into biohydrogen under mesophilic conditions. The discovery of strain <i>C</i><i>. populeti</i> FZ10 has special significance in the field of bioenergy.https://www.mdpi.com/2076-3417/12/19/9562<i>Clostridium populeti</i> FZ10isolation and identificationdark fermentationhydrogen productionlignocellulose |
| spellingShingle | Jingnan Zhang Baoxuan Jiang Haokun Zhang Sitong Qian Tao Wei Zhiping Zhang Lili Song Xu Yang Fermentative Hydrogen Production from Lignocellulose by Mesophilic <i>Clostridium populeti</i> FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated Compost Applied Sciences <i>Clostridium populeti</i> FZ10 isolation and identification dark fermentation hydrogen production lignocellulose |
| title | Fermentative Hydrogen Production from Lignocellulose by Mesophilic <i>Clostridium populeti</i> FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated Compost |
| title_full | Fermentative Hydrogen Production from Lignocellulose by Mesophilic <i>Clostridium populeti</i> FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated Compost |
| title_fullStr | Fermentative Hydrogen Production from Lignocellulose by Mesophilic <i>Clostridium populeti</i> FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated Compost |
| title_full_unstemmed | Fermentative Hydrogen Production from Lignocellulose by Mesophilic <i>Clostridium populeti</i> FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated Compost |
| title_short | Fermentative Hydrogen Production from Lignocellulose by Mesophilic <i>Clostridium populeti</i> FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated Compost |
| title_sort | fermentative hydrogen production from lignocellulose by mesophilic i clostridium populeti i fz10 newly isolated from microcrystalline cellulose acclimated compost |
| topic | <i>Clostridium populeti</i> FZ10 isolation and identification dark fermentation hydrogen production lignocellulose |
| url | https://www.mdpi.com/2076-3417/12/19/9562 |
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