Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatment
Abstract Background Polycystic ovary syndrome (PCOS) is the main cause of anovulatory infertility in women of reproductive age, and low-grade chronic inflammation plays a key role in the occurrence and development of PCOS. However, obesity, as a likely confounding factor, can affect the inflammatory...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12958-023-01183-6 |
| _version_ | 1797355456019038208 |
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| author | Jie Shang Siyu Wang Aiyuan Wang Fang Li Jing Zhang Jin Wang Rui Lv Haixia Chen Xiaohuan Mu Kai Zhang Xiaohong Bai Ye Tian |
| author_facet | Jie Shang Siyu Wang Aiyuan Wang Fang Li Jing Zhang Jin Wang Rui Lv Haixia Chen Xiaohuan Mu Kai Zhang Xiaohong Bai Ye Tian |
| author_sort | Jie Shang |
| collection | DOAJ |
| description | Abstract Background Polycystic ovary syndrome (PCOS) is the main cause of anovulatory infertility in women of reproductive age, and low-grade chronic inflammation plays a key role in the occurrence and development of PCOS. However, obesity, as a likely confounding factor, can affect the inflammatory state of PCOS patients. Objective The aim of this study was to comprehensively investigate intra-ovarian inflammatory states and their impact on embryo quality in PCOS patients with a normal BMI undergoing IVF treatment. Methods DIA-mass spectrometry-based proteomics and bioinformatic analysis were combined to comprehensively profile the protein expression of granulosa cells (GCs) from 5 normal-BMI PCOS patients and 5 controls. Thirty-four cytokines were further systematically detected in follicular fluid (FF) from 32 age- and BMI-matched normal-BMI patients using Luminex liquid chip suspension technology. Next, the differentially expressed cytokines were evaluated by enzyme-linked immunosorbent assay (ELISA) in 24 newly recruited subjects, and the relationship between these cytokines and embryo quality in PCOS patients was analysed. Finally, these cytokine levels were compared and evaluated in PCOS patients with different androgen levels. Results Proteomic analysis showed that the suppression of substance metabolism and steroid biosynthesis, more interestingly, resulted in an enhanced immune and inflammatory response in the GCs of normal-BMI PCOS patients and prompted the involvement of cytokines in this process. Luminex analysis further showed that FF macrophage inflammatory protein-1 beta (MIP-1β) and stromal cell-derived factor-1 alpha (SDF-1α) levels were significantly increased in normal-BMI PCOS patients compared to controls (P = 0.005; P = 0.035, respectively), and the ELISA results were consistent with these findings. Besides, FF MIP-1β showed an inverse correlation with the number of D3 good-quality embryos and the good-quality blastocyst rate in patients with PCOS (P = 0.006; P = 0.003, respectively), which remained significant after correction for multiple comparisons. Moreover, SDF-1α levels had no relationship with embryo development in PCOS patients. Additionally, SDF-1α levels were significantly lower in PCOS patients with high androgen levels than in controls (P = 0.031). Conclusions Local ovarian inflammation was present in normal-BMI PCOS patients, affecting follicular development, and FF MIP-1β may be a potential biomarker associated with embryo quality in normal-BMI PCOS patients. Graphical abstract |
| first_indexed | 2024-03-08T14:11:29Z |
| format | Article |
| id | doaj.art-b27b3554fb164e978c7a88cd67e57fdb |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-03-08T14:11:29Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-b27b3554fb164e978c7a88cd67e57fdb2024-01-14T12:42:47ZengBMCReproductive Biology and Endocrinology1477-78272024-01-0122111310.1186/s12958-023-01183-6Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatmentJie Shang0Siyu Wang1Aiyuan Wang2Fang Li3Jing Zhang4Jin Wang5Rui Lv6Haixia Chen7Xiaohuan Mu8Kai Zhang9Xiaohong Bai10Ye Tian11Department of Gynecology and Obstetrics, Tianjin Medical University General HospitalKey Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical UniversityKey Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical UniversityDepartment of Gynecology and Obstetrics, Tianjin Medical University General HospitalDepartment of Gynecology and Obstetrics, Tianjin Medical University General HospitalDepartment of Gynecology and Obstetrics, Tianjin Medical University General HospitalDepartment of Gynecology and Obstetrics, Tianjin Medical University General HospitalDepartment of Gynecology and Obstetrics, Tianjin Medical University General HospitalDepartment of Gynecology and Obstetrics, Tianjin Medical University General HospitalKey Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical UniversityDepartment of Gynecology and Obstetrics, Tianjin Medical University General HospitalDepartment of Gynecology and Obstetrics, Tianjin Medical University General HospitalAbstract Background Polycystic ovary syndrome (PCOS) is the main cause of anovulatory infertility in women of reproductive age, and low-grade chronic inflammation plays a key role in the occurrence and development of PCOS. However, obesity, as a likely confounding factor, can affect the inflammatory state of PCOS patients. Objective The aim of this study was to comprehensively investigate intra-ovarian inflammatory states and their impact on embryo quality in PCOS patients with a normal BMI undergoing IVF treatment. Methods DIA-mass spectrometry-based proteomics and bioinformatic analysis were combined to comprehensively profile the protein expression of granulosa cells (GCs) from 5 normal-BMI PCOS patients and 5 controls. Thirty-four cytokines were further systematically detected in follicular fluid (FF) from 32 age- and BMI-matched normal-BMI patients using Luminex liquid chip suspension technology. Next, the differentially expressed cytokines were evaluated by enzyme-linked immunosorbent assay (ELISA) in 24 newly recruited subjects, and the relationship between these cytokines and embryo quality in PCOS patients was analysed. Finally, these cytokine levels were compared and evaluated in PCOS patients with different androgen levels. Results Proteomic analysis showed that the suppression of substance metabolism and steroid biosynthesis, more interestingly, resulted in an enhanced immune and inflammatory response in the GCs of normal-BMI PCOS patients and prompted the involvement of cytokines in this process. Luminex analysis further showed that FF macrophage inflammatory protein-1 beta (MIP-1β) and stromal cell-derived factor-1 alpha (SDF-1α) levels were significantly increased in normal-BMI PCOS patients compared to controls (P = 0.005; P = 0.035, respectively), and the ELISA results were consistent with these findings. Besides, FF MIP-1β showed an inverse correlation with the number of D3 good-quality embryos and the good-quality blastocyst rate in patients with PCOS (P = 0.006; P = 0.003, respectively), which remained significant after correction for multiple comparisons. Moreover, SDF-1α levels had no relationship with embryo development in PCOS patients. Additionally, SDF-1α levels were significantly lower in PCOS patients with high androgen levels than in controls (P = 0.031). Conclusions Local ovarian inflammation was present in normal-BMI PCOS patients, affecting follicular development, and FF MIP-1β may be a potential biomarker associated with embryo quality in normal-BMI PCOS patients. Graphical abstracthttps://doi.org/10.1186/s12958-023-01183-6Polycystic ovary syndromeNormal BMIIntra-ovarian inflammatory statesEmbryo qualityIn vitro fertilization |
| spellingShingle | Jie Shang Siyu Wang Aiyuan Wang Fang Li Jing Zhang Jin Wang Rui Lv Haixia Chen Xiaohuan Mu Kai Zhang Xiaohong Bai Ye Tian Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatment Reproductive Biology and Endocrinology Polycystic ovary syndrome Normal BMI Intra-ovarian inflammatory states Embryo quality In vitro fertilization |
| title | Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatment |
| title_full | Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatment |
| title_fullStr | Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatment |
| title_full_unstemmed | Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatment |
| title_short | Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatment |
| title_sort | intra ovarian inflammatory states and their associations with embryo quality in normal bmi pcos patients undergoing ivf treatment |
| topic | Polycystic ovary syndrome Normal BMI Intra-ovarian inflammatory states Embryo quality In vitro fertilization |
| url | https://doi.org/10.1186/s12958-023-01183-6 |
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