| Summary: | Medicinal plants play important roles in traditional medicine, and numerous compounds among them have been recognized for their antimicrobial activity. However, little is known about the potential of Vietnamese medicinal plants for antifungal activity. In this study, we examined the antagonistic activity of twelve medicinal plant species collected in Northern Vietnam against <i>Penicillium digitatum</i>, <i>Aspergillus flavus</i>, <i>Aspergillus fumigatus</i>, and <i>Candida albicans</i>. The results showed that the antifungal activities of the crude extracts from <i>Mahonia bealei</i>, <i>Ficus semicordata</i>, and <i>Gnetum montanum</i> were clearly detected with the citrus postharvest pathogen <i>P. digitatum</i>. These extracts could fully inhibit the growth of <i>P. digitatum</i> on the agar medium, and on the infected citrus fruits at concentrations of 300–1000 µg/mL. Meanwhile, the other tested fungi were less sensitive to the antagonistic activity of the plant extracts. In particular, we found that the ethanolic extract of <i>M. bealei</i> displayed a broad-spectrum antifungal activity against all four pathogenic fungi. Analysis of this crude extract by enrichment coupled with high-performance liquid chromatography revealed that berberine and palmatine are major metabolites. Additional inspections indicated berberine as the key compound responsible for the antifungal activity of the <i>M. bealei</i> ethanolic extract. Our study provides a better understanding of the potential of Vietnamese medicinal plant resources for combating fungal pathogens. This work also highlights that the citrus pathogen <i>P. digitatum</i> can be employed as a model fungus for screening the antifungal activity of botanicals.
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