Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models
Abstract Introduction Excellent outcomes of arthroscopic rotator cuff repair for small and medium tears have been recently reported. However, re-tears after surgery have been a common complication after surgical repair of large and massive rotator cuff tears and often occur in early postoperative ph...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-10-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-021-02771-1 |
| _version_ | 1828119394588295168 |
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| author | Takeshi Kataoka Yutaka Mifune Atsuyuki Inui Hanako Nishimoto Takashi Kurosawa Kohei Yamaura Shintaro Mukohara Takehiko Matsushita Takahiro Niikura Yasuhiko Tabata Ryosuke Kuroda |
| author_facet | Takeshi Kataoka Yutaka Mifune Atsuyuki Inui Hanako Nishimoto Takashi Kurosawa Kohei Yamaura Shintaro Mukohara Takehiko Matsushita Takahiro Niikura Yasuhiko Tabata Ryosuke Kuroda |
| author_sort | Takeshi Kataoka |
| collection | DOAJ |
| description | Abstract Introduction Excellent outcomes of arthroscopic rotator cuff repair for small and medium tears have been recently reported. However, re-tears after surgery have been a common complication after surgical repair of large and massive rotator cuff tears and often occur in early postoperative phase. It was previously reported that basic fibroblast growth factor and platelet-rich plasma enhanced rotator cuff tear healing. We hypothesized that this combined therapy could enhance rotator cuff healing after rotator cuff repair in a rat model. This study aimed to evaluate the efficacy of combined therapy of platelet-rich plasma and basic fibroblast growth factor with gelatin-hydrogel sheet. Methods To create a rotator cuff defect, the infraspinatus tendon of Sprague Dawley rat was resected from the greater tuberosity. The infraspinatus tendons were repaired and covered with gelatin-hydrogel sheet impregnated with PBS (control group), basic fibroblast growth factor (bFGF group), platelet-rich plasma (PRP group), or both basic fibroblast growth factor and platelet-rich plasma (combined group). Histological examinations were conducted using hematoxylin and eosin, safranin O, and immunofluorescence staining, such as Isolectin B4, type II collagen at 2 weeks postoperatively. For mechanical analysis, ultimate failure load of the tendon-humeral head complex was evaluated at 6 weeks postoperatively. Results In the hematoxylin and eosin staining, the tendon maturing score of the combined group was higher than that of the control group at postoperative 2 weeks. In the safranin O staining, stronger proteoglycan staining was observed in the combined group compared with the other groups at postoperative 2 weeks. Vascular staining with isolectin B4 in 3 treatment groups was significantly higher than that in the control group. Type II collagen expression in the combined group was significantly higher than those in the other groups. The ultimate failure load of the combined group was significantly higher than that of the control group. Conclusion Combined therapy of basic fibroblast growth factor and platelet-rich plasma promoted angiogenesis, tendon maturing and fibrocartilage regeneration at the enthesis, which could enhance the mechanical strength. It was suggested that combined basic fibroblast growth factor and platelet-rich plasma might enhance both tendon and bone–tendon junction healing, and basic fibroblast growth factor and platelet-rich plasma might be synergistic. |
| first_indexed | 2024-04-11T13:47:37Z |
| format | Article |
| id | doaj.art-b27fb22ebcee41ae94b38751c7ab89a2 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T13:47:37Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-b27fb22ebcee41ae94b38751c7ab89a22022-12-22T04:20:55ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2021-10-011611910.1186/s13018-021-02771-1Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat modelsTakeshi Kataoka0Yutaka Mifune1Atsuyuki Inui2Hanako Nishimoto3Takashi Kurosawa4Kohei Yamaura5Shintaro Mukohara6Takehiko Matsushita7Takahiro Niikura8Yasuhiko Tabata9Ryosuke Kuroda10Department of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineLaboratory of Biomaterials, Institute for Frontier Life and Medical Sciences, Kyoto UniversityDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineAbstract Introduction Excellent outcomes of arthroscopic rotator cuff repair for small and medium tears have been recently reported. However, re-tears after surgery have been a common complication after surgical repair of large and massive rotator cuff tears and often occur in early postoperative phase. It was previously reported that basic fibroblast growth factor and platelet-rich plasma enhanced rotator cuff tear healing. We hypothesized that this combined therapy could enhance rotator cuff healing after rotator cuff repair in a rat model. This study aimed to evaluate the efficacy of combined therapy of platelet-rich plasma and basic fibroblast growth factor with gelatin-hydrogel sheet. Methods To create a rotator cuff defect, the infraspinatus tendon of Sprague Dawley rat was resected from the greater tuberosity. The infraspinatus tendons were repaired and covered with gelatin-hydrogel sheet impregnated with PBS (control group), basic fibroblast growth factor (bFGF group), platelet-rich plasma (PRP group), or both basic fibroblast growth factor and platelet-rich plasma (combined group). Histological examinations were conducted using hematoxylin and eosin, safranin O, and immunofluorescence staining, such as Isolectin B4, type II collagen at 2 weeks postoperatively. For mechanical analysis, ultimate failure load of the tendon-humeral head complex was evaluated at 6 weeks postoperatively. Results In the hematoxylin and eosin staining, the tendon maturing score of the combined group was higher than that of the control group at postoperative 2 weeks. In the safranin O staining, stronger proteoglycan staining was observed in the combined group compared with the other groups at postoperative 2 weeks. Vascular staining with isolectin B4 in 3 treatment groups was significantly higher than that in the control group. Type II collagen expression in the combined group was significantly higher than those in the other groups. The ultimate failure load of the combined group was significantly higher than that of the control group. Conclusion Combined therapy of basic fibroblast growth factor and platelet-rich plasma promoted angiogenesis, tendon maturing and fibrocartilage regeneration at the enthesis, which could enhance the mechanical strength. It was suggested that combined basic fibroblast growth factor and platelet-rich plasma might enhance both tendon and bone–tendon junction healing, and basic fibroblast growth factor and platelet-rich plasma might be synergistic.https://doi.org/10.1186/s13018-021-02771-1Rotator cuff tearBasic fibroblast growth factor (bFGF)Platelet-rich plasma (PRP)Rotator cuff repairRat modelInfraspinatus tendon |
| spellingShingle | Takeshi Kataoka Yutaka Mifune Atsuyuki Inui Hanako Nishimoto Takashi Kurosawa Kohei Yamaura Shintaro Mukohara Takehiko Matsushita Takahiro Niikura Yasuhiko Tabata Ryosuke Kuroda Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models Journal of Orthopaedic Surgery and Research Rotator cuff tear Basic fibroblast growth factor (bFGF) Platelet-rich plasma (PRP) Rotator cuff repair Rat model Infraspinatus tendon |
| title | Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models |
| title_full | Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models |
| title_fullStr | Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models |
| title_full_unstemmed | Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models |
| title_short | Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models |
| title_sort | combined therapy of platelet rich plasma and basic fibroblast growth factor using gelatin hydrogel sheet for rotator cuff healing in rat models |
| topic | Rotator cuff tear Basic fibroblast growth factor (bFGF) Platelet-rich plasma (PRP) Rotator cuff repair Rat model Infraspinatus tendon |
| url | https://doi.org/10.1186/s13018-021-02771-1 |
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