Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chicken
ABSTRACT: The Korat chicken (KR) is a slow-growing Thai chicken breed with relatively poor feed efficiency (FE) but very tasty meat with high protein and low fat contents, and a unique texture. To enhance the competitiveness of KR, its FE should be improved. However, selecting for FE has an unknown...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123002602 |
| _version_ | 1797802259993591808 |
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| author | Pramin Kaewsatuan Chotima Poompramun Satoshi Kubota Jirawat Yongsawatdigul Wittawat Molee Pekka Uimari Amonrat Molee |
| author_facet | Pramin Kaewsatuan Chotima Poompramun Satoshi Kubota Jirawat Yongsawatdigul Wittawat Molee Pekka Uimari Amonrat Molee |
| author_sort | Pramin Kaewsatuan |
| collection | DOAJ |
| description | ABSTRACT: The Korat chicken (KR) is a slow-growing Thai chicken breed with relatively poor feed efficiency (FE) but very tasty meat with high protein and low fat contents, and a unique texture. To enhance the competitiveness of KR, its FE should be improved. However, selecting for FE has an unknown effect on meat characteristics. Thus, understanding the genetic basis underlying FE traits and meat characteristics is needed. In this study, 75 male KR birds were raised up to 10 wk of age. For each bird, the feed conversion ratio (FCR), residual feed intake (RFI), and physicochemical properties, flavor precursors, and biological compounds in the thigh meat were evaluated. At 10 wk of age, thigh muscle samples from 6 birds (3 with high FCR and 3 with low FCR values) were selected, and their proteomes were investigated using a label-free proteomic method. Weighted gene coexpression network analysis (WGCNA) was used to screen the key protein modules and pathways. The WGCNA results revealed that FE and meat characteristics significantly correlated with the same protein module. However, the correlation was unfavorable; improving FE may result in a decrease in meat quality through the alteration in biological processes including glycolysis/gluconeogenesis, metabolic pathway, carbon metabolism, biosynthesis of amino acids, pyruvate metabolism, and protein processing in the endoplasmic reticulum. The hub proteins of the significant module (TNNT1, TNNT3, TNNI2, TNNC2, MYLPF, MYH10, GADPH, PGK1, LDHA, and GPI) were also identified to be associated with energy metabolism, and muscle growth and development. Given that the same proteins and pathways are present in FE and meat characteristics but in opposite directions, selection practices for KR should simultaneously consider both trait groups to maintain the high meat quality of KR while improving FE. |
| first_indexed | 2024-03-13T05:04:03Z |
| format | Article |
| id | doaj.art-b2832ffd4596469f8695f487a979ed79 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-13T05:04:03Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-b2832ffd4596469f8695f487a979ed792023-06-17T05:17:24ZengElsevierPoultry Science0032-57912023-07-011027102741Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chickenPramin Kaewsatuan0Chotima Poompramun1Satoshi Kubota2Jirawat Yongsawatdigul3Wittawat Molee4Pekka Uimari5Amonrat Molee6School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandDepartment of Agricultural Sciences, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki 00790, FinlandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand; Corresponding author:ABSTRACT: The Korat chicken (KR) is a slow-growing Thai chicken breed with relatively poor feed efficiency (FE) but very tasty meat with high protein and low fat contents, and a unique texture. To enhance the competitiveness of KR, its FE should be improved. However, selecting for FE has an unknown effect on meat characteristics. Thus, understanding the genetic basis underlying FE traits and meat characteristics is needed. In this study, 75 male KR birds were raised up to 10 wk of age. For each bird, the feed conversion ratio (FCR), residual feed intake (RFI), and physicochemical properties, flavor precursors, and biological compounds in the thigh meat were evaluated. At 10 wk of age, thigh muscle samples from 6 birds (3 with high FCR and 3 with low FCR values) were selected, and their proteomes were investigated using a label-free proteomic method. Weighted gene coexpression network analysis (WGCNA) was used to screen the key protein modules and pathways. The WGCNA results revealed that FE and meat characteristics significantly correlated with the same protein module. However, the correlation was unfavorable; improving FE may result in a decrease in meat quality through the alteration in biological processes including glycolysis/gluconeogenesis, metabolic pathway, carbon metabolism, biosynthesis of amino acids, pyruvate metabolism, and protein processing in the endoplasmic reticulum. The hub proteins of the significant module (TNNT1, TNNT3, TNNI2, TNNC2, MYLPF, MYH10, GADPH, PGK1, LDHA, and GPI) were also identified to be associated with energy metabolism, and muscle growth and development. Given that the same proteins and pathways are present in FE and meat characteristics but in opposite directions, selection practices for KR should simultaneously consider both trait groups to maintain the high meat quality of KR while improving FE.http://www.sciencedirect.com/science/article/pii/S0032579123002602feed efficiencymeat characteristicshub proteinsWGCNAslow-growing chicken |
| spellingShingle | Pramin Kaewsatuan Chotima Poompramun Satoshi Kubota Jirawat Yongsawatdigul Wittawat Molee Pekka Uimari Amonrat Molee Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chicken Poultry Science feed efficiency meat characteristics hub proteins WGCNA slow-growing chicken |
| title | Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chicken |
| title_full | Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chicken |
| title_fullStr | Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chicken |
| title_full_unstemmed | Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chicken |
| title_short | Thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow-growing chicken |
| title_sort | thigh muscle metabolic response is linked to feed efficiency and meat characteristics in slow growing chicken |
| topic | feed efficiency meat characteristics hub proteins WGCNA slow-growing chicken |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123002602 |
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