Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605
Pseudomonas syringae pv. tabaci 6605 has two multidrug resistance (MDR) efflux pump transporters, MexAB-OprM and MexEF-OprN. To understand the role of these MDR efflux pumps in virulence, we generated deletion mutants, ΔmexB, ΔmexF, and ΔmexBΔmexF, and investigated their sensitivity to plant-derived...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2020-04-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143514 |
| _version_ | 1818340155252015104 |
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| author | Yuki Ichinose Takafumi Nishimura Minori Harada Ryota Kashiwagi Mikihiro Yamamoto Yoshiteru Noutoshi Kazuhiro Toyoda Fumiko Taguchi Daigo Takemoto Hidenori Matsui |
| author_facet | Yuki Ichinose Takafumi Nishimura Minori Harada Ryota Kashiwagi Mikihiro Yamamoto Yoshiteru Noutoshi Kazuhiro Toyoda Fumiko Taguchi Daigo Takemoto Hidenori Matsui |
| author_sort | Yuki Ichinose |
| collection | DOAJ |
| description | Pseudomonas syringae pv. tabaci 6605 has two multidrug resistance (MDR) efflux pump transporters, MexAB-OprM and MexEF-OprN. To understand the role of these MDR efflux pumps in virulence, we generated deletion mutants, ΔmexB, ΔmexF, and ΔmexBΔmexF, and investigated their sensitivity to plant-derived antimicrobial compounds, antibiotics, and virulence. Growth inhibition assays with KB soft agar plate showed that growth of the wild-type (WT) was inhibited by 5 μl of 1 M catechol and 1 M coumarin but not by other plant-derived potential antimicrobial compounds tested including phytoalexins. The sensitivity to these compounds tended to increase in ΔmexB and ΔmexBΔmexF mutants. The ΔmexBΔmexF mutant was also sensitive to 2 M acetovanillone. The mexAB-oprM was constitutively expressed, and activated in the ΔmexF and ΔmexBΔmexF mutant strains. The swarming and swimming motilities were impaired in ΔmexF and ΔmexBΔmexF mutants. The flood inoculation test indicated that bacterial populations in all mutant strains were significantly lower than that of WT, although all mutants and WT caused similar disease symptoms. These results indicate that MexAB-OprM extrudes plant-derived catechol, acetovanillone, or coumarin, and contributes to bacterial virulence. Furthermore, MexAB-OprM and MexEF-OprN complemented each other’s functions to some extent. |
| first_indexed | 2024-12-13T15:38:24Z |
| format | Article |
| id | doaj.art-b2834e93c53f4020a02f379d88525b9d |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-13T15:38:24Z |
| publishDate | 2020-04-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-b2834e93c53f4020a02f379d88525b9d2022-12-21T23:39:55ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542020-04-0136214815610.5423/PPJ.OA.11.2019.027310.5423PPJ.OA.11.2019.0273Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605Yuki Ichinose0Takafumi Nishimura1Minori Harada2Ryota Kashiwagi3Mikihiro Yamamoto4Yoshiteru Noutoshi5Kazuhiro Toyoda6Fumiko Taguchi7Daigo Takemoto8Hidenori Matsui9Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanFaculty of Agriculture, Okayama University, Okayama 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanGraduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanPseudomonas syringae pv. tabaci 6605 has two multidrug resistance (MDR) efflux pump transporters, MexAB-OprM and MexEF-OprN. To understand the role of these MDR efflux pumps in virulence, we generated deletion mutants, ΔmexB, ΔmexF, and ΔmexBΔmexF, and investigated their sensitivity to plant-derived antimicrobial compounds, antibiotics, and virulence. Growth inhibition assays with KB soft agar plate showed that growth of the wild-type (WT) was inhibited by 5 μl of 1 M catechol and 1 M coumarin but not by other plant-derived potential antimicrobial compounds tested including phytoalexins. The sensitivity to these compounds tended to increase in ΔmexB and ΔmexBΔmexF mutants. The ΔmexBΔmexF mutant was also sensitive to 2 M acetovanillone. The mexAB-oprM was constitutively expressed, and activated in the ΔmexF and ΔmexBΔmexF mutant strains. The swarming and swimming motilities were impaired in ΔmexF and ΔmexBΔmexF mutants. The flood inoculation test indicated that bacterial populations in all mutant strains were significantly lower than that of WT, although all mutants and WT caused similar disease symptoms. These results indicate that MexAB-OprM extrudes plant-derived catechol, acetovanillone, or coumarin, and contributes to bacterial virulence. Furthermore, MexAB-OprM and MexEF-OprN complemented each other’s functions to some extent.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143514acetovanillonecatecholcoumarinmexab-oprmvirulence |
| spellingShingle | Yuki Ichinose Takafumi Nishimura Minori Harada Ryota Kashiwagi Mikihiro Yamamoto Yoshiteru Noutoshi Kazuhiro Toyoda Fumiko Taguchi Daigo Takemoto Hidenori Matsui Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605 The Plant Pathology Journal acetovanillone catechol coumarin mexab-oprm virulence |
| title | Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605 |
| title_full | Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605 |
| title_fullStr | Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605 |
| title_full_unstemmed | Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605 |
| title_short | Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605 |
| title_sort | role of two sets of rnd type multidrug efflux pump transporter genes mexab oprm and mexef oprn in virulence of pseudomonas syringae pv tabaci 6605 |
| topic | acetovanillone catechol coumarin mexab-oprm virulence |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143514 |
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