Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth
Abstract Ureaplasma and Prevotella infections are well-known bacteria associated with preterm birth. However, with the development of metagenome sequencing techniques, it has been found that not all Ureaplasma and Prevotella colonizations cause preterm birth. The purpose of this study was to determi...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13871-1 |
| _version_ | 1818236862447222784 |
|---|---|
| author | Sunwha Park Young-Ah You Young-Han Kim Eunjin Kwon AbuZar Ansari Soo Min Kim Gain Lee Young Min Hur Yun Ji Jung Kwangmin Kim Young Ju Kim |
| author_facet | Sunwha Park Young-Ah You Young-Han Kim Eunjin Kwon AbuZar Ansari Soo Min Kim Gain Lee Young Min Hur Yun Ji Jung Kwangmin Kim Young Ju Kim |
| author_sort | Sunwha Park |
| collection | DOAJ |
| description | Abstract Ureaplasma and Prevotella infections are well-known bacteria associated with preterm birth. However, with the development of metagenome sequencing techniques, it has been found that not all Ureaplasma and Prevotella colonizations cause preterm birth. The purpose of this study was to determine the association between Ureaplasma and Prevotella colonization with the induction of preterm birth even in the presence of Lactobacillus. In this matched case–control study, a total of 203 pregnant Korean women were selected and their cervicovaginal fluid samples were collected during mid-pregnancy. The microbiome profiles of the cervicovaginal fluid were analyzed using 16S rRNA gene amplification. Sequencing data were processed using QIIME1.9.1. Statistical analyses were performed using R software, and microbiome analysis was performed using the MicrobiomeAnalyst and Calypso software. A positive correlation between Ureaplasma and other genera was highly related to preterm birth, but interestingly, there was a negative correlation with Lactobacillus and term birth, with the same pattern observed with Prevotella. Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth, although Ureaplasma and Prevotella are associated with preterm birth. Balanced colonization between Lactobacillus and Ureaplasma and Prevotella is important to prevent preterm birth. |
| first_indexed | 2024-12-12T12:16:36Z |
| format | Article |
| id | doaj.art-b2846f3f090148b689c65c2a526b4845 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T12:16:36Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b2846f3f090148b689c65c2a526b48452022-12-22T00:24:45ZengNature PortfolioScientific Reports2045-23222022-06-011211910.1038/s41598-022-13871-1Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birthSunwha Park0Young-Ah You1Young-Han Kim2Eunjin Kwon3AbuZar Ansari4Soo Min Kim5Gain Lee6Young Min Hur7Yun Ji Jung8Kwangmin Kim9Young Ju Kim10Department of Obstetrics and Gynecology, College of Medicine, Ewha Medical Research Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine, Ewha Medical Research Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine, Yonsei UniversityDepartment of Obstetrics and Gynecology, College of Medicine, Ewha Medical Research Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine, Ewha Medical Research Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine, Ewha Medical Research Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine, Ewha Medical Research Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine, Ewha Medical Research Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine, Yonsei University3BIGS CO., LTD.Department of Obstetrics and Gynecology, College of Medicine, Ewha Medical Research Institute, Ewha Womans UniversityAbstract Ureaplasma and Prevotella infections are well-known bacteria associated with preterm birth. However, with the development of metagenome sequencing techniques, it has been found that not all Ureaplasma and Prevotella colonizations cause preterm birth. The purpose of this study was to determine the association between Ureaplasma and Prevotella colonization with the induction of preterm birth even in the presence of Lactobacillus. In this matched case–control study, a total of 203 pregnant Korean women were selected and their cervicovaginal fluid samples were collected during mid-pregnancy. The microbiome profiles of the cervicovaginal fluid were analyzed using 16S rRNA gene amplification. Sequencing data were processed using QIIME1.9.1. Statistical analyses were performed using R software, and microbiome analysis was performed using the MicrobiomeAnalyst and Calypso software. A positive correlation between Ureaplasma and other genera was highly related to preterm birth, but interestingly, there was a negative correlation with Lactobacillus and term birth, with the same pattern observed with Prevotella. Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth, although Ureaplasma and Prevotella are associated with preterm birth. Balanced colonization between Lactobacillus and Ureaplasma and Prevotella is important to prevent preterm birth.https://doi.org/10.1038/s41598-022-13871-1 |
| spellingShingle | Sunwha Park Young-Ah You Young-Han Kim Eunjin Kwon AbuZar Ansari Soo Min Kim Gain Lee Young Min Hur Yun Ji Jung Kwangmin Kim Young Ju Kim Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth Scientific Reports |
| title | Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth |
| title_full | Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth |
| title_fullStr | Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth |
| title_full_unstemmed | Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth |
| title_short | Ureaplasma and Prevotella colonization with Lactobacillus abundance during pregnancy facilitates term birth |
| title_sort | ureaplasma and prevotella colonization with lactobacillus abundance during pregnancy facilitates term birth |
| url | https://doi.org/10.1038/s41598-022-13871-1 |
| work_keys_str_mv | AT sunwhapark ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT youngahyou ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT younghankim ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT eunjinkwon ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT abuzaransari ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT soominkim ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT gainlee ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT youngminhur ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT yunjijung ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT kwangminkim ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth AT youngjukim ureaplasmaandprevotellacolonizationwithlactobacillusabundanceduringpregnancyfacilitatestermbirth |